I use zeiss lsm 880 and when performing lamda scan(400-700nm), the software scans each channel (like 32 images 10nm apart) one after another. However, in your video I could see all spectral images scanned simultaneously at the real time. How should i fix this?
@AlyonaMinina
8 жыл бұрын
We have 34 detectors in our LSM780. The LSM 880 should be the same in the main scan head, take a look here at the 0:32 : kzitem.info/news/bejne/sqCB2ICKgXmflm0 the 32-channel GaAsP detector = Quasar detector = ChS in our LSM780= 32 individual detectors put in a row, each individual detector can catch an 8.9nm range of the visible spectrum. So in one go you can detect 32x8.9= 284.8 nm, which is approximately 700-400 nm you are trying to catch. I suspect, what you are doing, is using a single detector (Ch1 or Ch2) and then you narrow down the range of wavelengths they receive to 10nm by pushing the sliders together. There is an interactive cartoon here (please note, the cartoon is not very accurate, you can actually deviate the whole spectrum to the Ch1 or Ch2.): zeiss-campus.magnet.fsu.edu/tutorials/spectralimaging/quasar34ch/indexflash.html. On the cartoon try to push the right and left sliders together until the gap between them is very narrow and then move them both to the left or right without changing the size of the gap. You will see what happens, when the software predicts number of required passes more than 1: you can detect different small ranges of the spectrum, but not simultaneously, because you need to move sliders To have detection of the spectrum in one go: 1. Make sure, that detection happens on the Quasar detector (do you have a technician to help you with it?). At 1:37 you can see that I am using ChS to detect 410-695 nm with 8.9 nm step 2. Make sure that the step is set to 8.9nm or more 3. number of passes should be automatically calculated as one (# passes 1)
@FirstPersonSciencePodcast
4 жыл бұрын
@@AlyonaMinina thank you so much for taking the time to explain and post these videos. More helpful than any other source on the internet, by far. You've helped me go from a complete novice, to teaching the Technicians in just a few days. You rock!!
@AlyonaMinina
4 жыл бұрын
First-Person Science thanks! i am very happy the videos are useful))
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