Slides for this talk can be downloaded here: drive.google.c...
As part of the B.Sc. Honours program at the Biotechnology Department at University of the Western Cape, I edited this lecture in the Clinical Biomarkers module to explain the relationship between proteomic discovery experiments and proteomic targeted verification experiments. I noted the role played by 2D Gel Electrophoresis, but most of the talk was based in fractionated samples paired with LC-MS/MS. This lecture differs from my normal set of lectures on proteomics in that it emphasized the value of Selected Reaction Monitoring (SRM) experiments using triple quadrupole mass spectrometers. They represent one of the best ways to derive robust quantitation for peptides in targeted proteins.
Негізгі бет Ғылым және технология 20230821 Proteomics for Clinical Biomarkers
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