For more information please go to our website:
www.abcreagents.com
Materials needed:
- Concentrate kit (“1 gal”) from ABC Reagents
o Will make 2X gal A, 1 gal B, 1 gal C
- Funnel will be useful
- Will need 4 x 1 gallon of distilled water
- Pour the water into larger container as you will need to start with 4 empty 1 gallon containers
- Containers must be made of HDPE (or glass)
Contact us for common HDPE container sources if needed
Reagent A: THIS WILL BE REPEATED 2X
1. In an empty 1 gallon container, fill the container ~ half way with distilled water (OR pour out ~ half of a new, filled 1 gal distilled H2O container)
2. Pour ALL contents of Reagent A #1 into container.
3. Rinse Reagent A #1 container several times (3X with ~50-100mL each time) with distilled water and pour into the gallon container.
4. Fill the 1 gallon container with distilled water ~ ¾ full, mix well
5. Pour Reagent A #2 into the 1 gallon container
6. Rinse Reagent A #2 container (2X with ~50mL) with distilled water and pour into 1 gallon container, mix well.
7. Fill remainder of 1 gallon container with distilled water.
Fill to top. Very little air gap should be visible, just below cap
MIX WELL!!
Rinse Funnel before next step
Reagent B
1. In an empty 1 gallon container, fill the container ~ half way with distilled water. (OR pour out ~ half of a new, filled 1 gal distilled H2O container)
2. Pour ALL contents of Reagent B #1 into container.
3. Rinse 2X Reagent B #1 bottle with ~50-100mL distilled water and pour into 1 gallon container. Mix well
4. Pour Reagent B #2 into 1 gallon container
5. Rinse 2X Reagent B #2 bottle with ~50-100mL distilled water and pour into 1 gallon container. Mix well
6. Pour Reagent B #3 into 1 gallon container
7. Rinse 2X Reagent B #3 bottle with ~50-100mL distilled water and pour into 1 gallon container. Mix well.
8. Fill remainder of 1 gallon container with distilled water.
Fill to top. Very little air gap should be visible, ~ 1” just below cap.
MIX WELL!!
Reagent C
1. In an empty 1 gallon container, fill the container ~ half way with distilled water. (OR pour out ~ half of a new, filled 1 gal distilled H2O container)
2. Pour ALL contents of Reagent C #1 into container. Mix well.
3. Rinse 2X Reagent C #1 bottle with ~50-100mL distilled water and pour into 1 gallon container. Mix well.
4. Pour Reagent C #2 into 1 gallon container. Mix well.
5. Rinse 2X Reagent C #2 bottle with ~50-100mL distilled water and pour into 1 gallon container. Mix well.
6. Add distilled water until approximately ¾ of container is full. MIX!!!
7. Fill remainder of 1 gallon container with distilled water.
Fill to top. Very little air gap should be visible, just below cap
MIX WELL, again!!!!
Негізгі бет Ойын-сауық ABC Reagents - How to Prepare the 1 Gallon Concentration Kit
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