Trying to express a protein in bacteria and it isn’t going well? Maybe you should try expression in an insect cell! Getting the protein recipe in there takes a bit more work but the Baculovirus Expression Vector System (BEVS) is worth it if e. coli make your protein go berserk! Since all organisms, from bacteria to insects to mice to humans can read the same genetic language, you can stick the DNA instructions for making a protein into any type of cell and they can turn it into protein (we call this recombinant protein expression)- but just because they can doesn’t mean they will and just because they will doesn’t mean they’ll do it *well*! Oftentimes proteins that are more “complex” and/or require helper molecules to fold require more “complex” cells for proper expression and insect cell lines like Sf9 and Hi5 can provide that complexity needed for your protein to thrive! (at a lower cost and often higher yield than mammalian cells)⠀
text and graphics: bit.ly/baculovirusexpression
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Негізгі бет Ғылым және технология Baculovirus Expression Vector Systems (getting insect cells to make protein for us)
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