I also did it as ratio: .3 / 0.41 = x / 0. 47 .... .3(.47) / .41 = .34 as well. If we are within are solution standards and we have a straight line we should be good this way as well
@fabioverdi6291
3 жыл бұрын
Help with a question ????? A compound absorb light in 227 nm. The standard curve calibration for the compound is Abs = 372x + 0,6432 which the concentration is g/100mL. The technique was analytical standard adding from fluoxetine hydrochloride (C17H18F3NO.HCl - molar mass = 345,79 g/mol). So determine the concentration in mol/L from the analysed solution. Show all calcs progression.
@wartburgchemistry6868
3 жыл бұрын
Hello! I think that there is a piece of data missing in your problem. Was the absorbance of the analyzed solution given?
@solarityrealms3666
2 жыл бұрын
@@wartburgchemistry6868 bruh that’s his homework xD
@wartburgchemistry6868
2 жыл бұрын
@@solarityrealms3666 Yea...figured as much. :)
@flower_girl4983
9 ай бұрын
Can you do beer-lambert law to determine the concentration, while absorbance is being measured using spectrometry
@anonymous-user-2024
4 жыл бұрын
Thank you for the post. How can the Beer-Lanbert's law be used when determining the specific activity of an enzyme (a measure of the purity of an enzyme)?
@wartburgchemistry6868
4 жыл бұрын
Are you looking to quantitiate the amount of the enzyme or to just see how fast it is acting? If you are looking for how fast, you could follow either the increase or decrease in the absorbance of the solution over time. This assumes that there is a species in solution (reactant or product) with the enzyme that absorbs some unique wavelength of light. Otherwise, if you're just looking for how much enzyme is in solution you could either create a standard curve or look up the molar absorptivity of the enzyme.
@ingFS
2 жыл бұрын
Really helpful! Keep up the god work. nice job man.
@aleyzgoneloco123clap
2 жыл бұрын
where did that equation 1.4x-0.01 come from? Also R^2=1 ?
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