What is the procedure for qualitative estimation of proteins by Bradford assay?
@eunicetan5960
4 жыл бұрын
what kind of non covalent bonding the BSA binds to protein?can you explain what happens during the incubation time,could you explain the mechanism?why does it cause a shift in maximal absorbance?
@alisaguadarroma829
4 жыл бұрын
vanderwalls and ionic bonding is taking place, the bradford reagent interacts with the protein linearly. such that more protein in the sample results in more non-covalen bonding between the dye and the protein. for more detailed info on the principle behind this mechanism see en.wikipedia.org/wiki/Bradford_protein_assay
@eunicetan5960
4 жыл бұрын
@@alisaguadarroma829 thankyou so much!!
@sinaerfani2375
5 жыл бұрын
Is the Phosphate buffered saline a mandatory buffer to use, i don't have that buffer. If i don't use it what will happen to the pH, and is their any other buffer i can use.
@adronung1892
4 жыл бұрын
It just matters that you use the same buffer as your sample is in. So your control is just the buffer.
@someoneOnYoutube001
5 жыл бұрын
Does it matter if I use a 3.5 ml cuvette? Will it affect the absorbance?
@felipedejesusbonillaahumad7415
Жыл бұрын
Need to read: "Lambert-Beer law" in order to understand the correlation between absorbance, concentration and dilution factor.
@sofiaolivares972
3 жыл бұрын
i want to estimate the protein of pollen, does anyone have a procedure or info to help??
@amiraalmahrazi1816
3 жыл бұрын
What is the purpose of standard,I couldn’t get it,please explain to me!?
@londonberry2180
3 жыл бұрын
basically, you can't directly quantify the amount of protein in an unknown sample. You need to do a relative quantification - which means by using a series of known protein concentration, you obtain a set of absorbance values which are arranged to form a standard curve. Then, the standard curve is used to indirectly quantify the amount of protein you have in your unknown sample based on its absorbance values.
@nwanduchikeluba4094
3 жыл бұрын
For a coloured protein binding sample such as polysaccharide of inonotus obliquus, which is usually brown in colour. How should I approach it since it is not white in colour such as the milk.
@londonberry2180
3 жыл бұрын
make a 10x - 20x dilution or something, quantify it, before multiplying back by the dilution factor. Once you do the dilution, the colour should be less prominent.
@isabellek1171
6 жыл бұрын
Will this work if I want to test protein concentration in soy milk?
@isabellek1171
6 жыл бұрын
@@thamizh2850 Thank you
@isabellek1171
6 жыл бұрын
@@thamizh2850 What do you mean by linear range? So the concentration of protein in the soy milk is enough for the Bradford Assay and spectrophotometer to work?
@gowthamvenkatchalam5312
5 жыл бұрын
Can we have a sprout seed with protein concentration of 55 mg/ml?
@fatimatoheed6542
5 жыл бұрын
@@thamizh2850 will this method work for the determination of protein in food such as rice or wheat?
@fatimatoheed6542
5 жыл бұрын
@@thamizh2850 thanks
@shwethaguled9518
4 жыл бұрын
I want to estimate the protein from the fish sample.. Can u suggest for me procedure plzzz...
Пікірлер: 27