Dude you are awesome, this is a really great and helpful set of informative and detailed videos for such a difficult to expand class of cacti. I really appreciate you doing this. Since I own most of this equipment already, I couldn't be more excited to start trying this.
@yibofan6855
Ай бұрын
Thank you for posting this. It seems like an open flame that torches the containers' brims was unnecessary and it's okay to hover over the explants. I was taught with these cautions in school but I want to improve my efficiency by simplifying the process. This video is informative.
@carlholdt1042
Жыл бұрын
Great video! Most important question: what medium do you use for this?
@cactifanatici
10 ай бұрын
MS based
@gios.5816
9 ай бұрын
great job, when using the MS medium do you happen to add any other growing hormones for specific growth? would you suggest starting out at half strength ms powder? good looking out.
@cactifanatici
9 ай бұрын
Honestly for cactus they don’t require any hormones once they get going but I do add to speed up things
@jst3455
2 жыл бұрын
So if I were to TC dragon fruit varieties, would my explants need the actual stem from the middle, or just the spines where new growth occurs? Are bigger or smaller chunks better? Do you know if contaminants can get inside the container if it's sealed? Thanks so much for this video. It has helped me tremendously.
@cactifanatici
2 жыл бұрын
The smaller the piece contains the meristem the better but on the other hand having more tissue they survives with the meristem encourages faster growth. More tissue increase contamination chances. No they contamination does not get through. All containers are wrapped with micropore tape of moving to regular non clean room environment
@Someone-bx1sc
2 жыл бұрын
Question, lophophoras are painfully slow in their own roots, This accelerates that or just helps with propagation? Also you should try tissue culture with seeds
@cactifanatici
2 жыл бұрын
Yes I have over 100 started from seed. They are very fast in TC compared to regular in my opinion but still not as fast as a pereskiopsis graft
@BobSacamano666
2 жыл бұрын
It's would be interesting to know if alkaloid content differs or if it's just water weight.
@ThirdEyeKultivations
Жыл бұрын
What do you call your containers you put ur TC in or where did you purchase them?
@cactifanatici
10 ай бұрын
Sterile deli containers
@nealjones76
6 ай бұрын
Loved the video, did you trim the explant material that was exposed to bleach after rinsing, before cutting down into smaller pieces?
@fbt2007
2 жыл бұрын
One of the best videos on plant tissue culture procedures where everything is clearly explained that I have seen on KZitem 👍
@carolbrinkley6189
6 ай бұрын
How do you cut up a leaf from a Thanksgiving cactus?
@dbfarm8974
2 жыл бұрын
Thank you so much for sharing this info! Slowly making the transition from growing culinary mushroom culture (reishi, lions mane, oysters) to plant tissue culture. Rare to find these helpful videos for cacti plant tissue culture techniques! Great job and very informative! Hope to find some MS cacti media (and woody plant culture) recipe videos someday :-)
@cactifanatici
2 жыл бұрын
Regular base media works for almost everything. Some special medias work better or just marketing
@TheRootedPepper.Slugwell
2 ай бұрын
@cactiFanatic do you have a list of hormones and where to get them. Such as a package deal where I can buy them all? I’m not tech savy so I don’t really know much out online stuff. Took me forever to find this video again. And another couple days to figure out how to comment on KZitem. Do you have an email?
@cactifanatici
2 ай бұрын
Yeah email me at cactifanatici@gmail.com My patreon has that list and info
@merlinaroyeras4581
2 жыл бұрын
I would suggest if you use a sterile 240ml wide mouth bottle with a cap so you can rinse your explant with dist H2O 3x by shaking then decant the water thoroughly. You have too many paper on top of the bench. Cut it into smaller pieces bond paper size (sterile) to carry out your disection of the explant if you do not want to use a petri dish. Bec us we are used to petri dish. There is an effect on the growth of the explant with the size of the tissue vs container to volume ratio of culture medium. You need to maximize the growth in short period of time.
@cactifanatici
2 жыл бұрын
To each there own. I have many many many vessels. That’s a sterile drape.
@Mycoheterotroph
4 ай бұрын
Do you use PGRs in your media? I’m about to start a large tissue culture operation of palo santo but definitely want to branch out into xerophiles and carnivores, and for the palo santo I will be trying somatic embryogenesis and from there using a bunch of pgrs to pump shoots, etc
@cactifanatici
4 ай бұрын
Sometimes on my patreon I go more into the hormones and when I’m using them
@socks982
6 ай бұрын
Where do we buy those containers?
@richardgoforth
11 ай бұрын
What about the media. I want to clone Trichocereus cacti. I get the basic idea. I am unsure what media. Is there a protocal for cloning cactus that is not behind a paywall?
@cactifanatici
10 ай бұрын
Yeah!! It’s online free. Just use a MS based media with vitamins, 30g sugar, activated charcoal and agar to set. That’s good enough for basic tissue culture then maybe BAP to induce
@MycoTrophic
2 жыл бұрын
dude i love this!
@Steve-cn5up
2 жыл бұрын
This was an amazing video! You did an excellent job of covering all the details. I look forward to more of your videos on this subject.
@Meatmust
2 жыл бұрын
Awesome videos! Finally someone demonstrating tissue culture on cacti. I can't wait for your next video.
@LarsLarsen77
Жыл бұрын
I've watched literally every video you've made. I still have no idea how to TC cacti. What's your multiplication media? Nobody knows!
@LarsLarsen77
10 ай бұрын
*brings up sci-hub*
@robinsontroncos9266
2 жыл бұрын
Thanks for sharing your knowledge,
@claudekim7876
Жыл бұрын
so u can do this with any other cacti? can i do this with like copiapoa cinerea?
@cactifanatici
Жыл бұрын
Just posted on my Patreon for copiapoa ;)
@xoxoalyssaxoxo12
2 жыл бұрын
I’ve been waiting for you to post more videos! lol i love watching your content. I have access to a 7 year old lop and i was wondering if you think it’s still possible for me to clone it?
@cactifanatici
2 жыл бұрын
Yeah any age can be done but the older the harder because it breaks my heart haha
@TheBaerserka
Жыл бұрын
One day ago i thought the same about sissors :D Great videos thx
@PatriotMango
2 жыл бұрын
appreciate the video. for dragonfruit, can you only use the spines and not the rest of the plant?
@cactifanatici
2 жыл бұрын
You could but it’s very long and difficult path. Best to use nodal / meristematic tissue like Spines / aeroles in cactus
@cjc3065
Жыл бұрын
Do you use some equipment like exhaust ventilation to be sterile?
@cactifanatici
Жыл бұрын
I have a FFU but the work can be done in a still air box set up also
@junohenshaw6434
2 жыл бұрын
What's your media recipe?
@cactifanatici
2 жыл бұрын
Not going to wine and dine me first!!! Lol It’s MS base media with vitamins : classic recipe
@junohenshaw6434
2 жыл бұрын
@@cactifanatici Oh I happily would lmao! Thank you!
@rahulramesh8714
2 жыл бұрын
MS with 1mg BAP ..
@Thesacrednetwork
Жыл бұрын
I was told you don't need it if you are using the Gamborg B5. The media is for callous plants like most cacti. 👍😁
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