Very helpful! I understood CLB method very well! Thank you! 🙏♥️🌼
@MissSkello
2 жыл бұрын
I'm glad!! ❤️
@mfbvlog
4 жыл бұрын
I come randomly then I see video Full satisfaction I got
@MissSkello
4 жыл бұрын
thank you!!
@chandanbuchandanbu8686
3 жыл бұрын
Thank you so much maam.. now,I cleared all my doubts of this concept very fine...🥳🥳🥳
@aarondesalvio
3 жыл бұрын
Incredibly helpful, thank you!
@pragyarawat6759
Жыл бұрын
Thank you for the video.If possible kindly make one video on Muller-5 method.
@TheHope045
11 ай бұрын
Mam plz 🙏🏻 vdo on theee 3 topics .. there is no vdo related to these in yt .. 🙏🏻 1.Antibiotic resistance in chlamydomonas 2.Mitochondrial mutation in saccharomyces 3. infective heridity in paramecium
@MissSkello
11 ай бұрын
ok sure! I'll try to make it soon
@TheHope045
11 ай бұрын
@@MissSkello ty mam 🙏🏻
@ipsitkumarnaik8151
3 жыл бұрын
Thank u mam for help this topic.
@payalpatra2052
19 күн бұрын
Thank you for saving me an hour! 😁
@aathirareghu4093
3 жыл бұрын
Thank you mam... I have a presentation today... And I was really confused
@MissSkello
3 жыл бұрын
I'm glad it helped ❤️
@mdsaquib3057
3 жыл бұрын
Now doubt cleared, thank u so much.😘😘😍😍
@MDSUMONHOSSAIN-m4j
11 ай бұрын
Nice explanation, Good work Mam, Carey On
@MissSkello
11 ай бұрын
thank you
@RWC708
26 күн бұрын
thanks a lot... it's 2024... Watching from BD.
@MissSkello
26 күн бұрын
Glad to know I could help!
@GScCollege
19 күн бұрын
maam , Is CLB an artificial chromosome , which conatain three genes i.e. C, L, B gene , constructed by scientist ?
@snehasybiswas5759
2 жыл бұрын
Amazing mam thanks for thi video
@dhruvuppal4305
3 жыл бұрын
Thanku ma'am really cleared my concepts
@satyakimandal308
4 ай бұрын
Thank you so much ❤
@bedamatiray8712
Жыл бұрын
Nice 🙏
@xolor2983
3 ай бұрын
Thank you mam
@andrasbajusz9290
Жыл бұрын
thank you
@scienceexperiments9008
Жыл бұрын
Very nicely explained
@primabagui3137
4 жыл бұрын
Thank you so much mam❤️❤️❤️❤️. It is really helpful.😍
@MissSkello
4 жыл бұрын
I'm glad it helped! Thank you❤️
@healthwealth-265
3 жыл бұрын
Madam you explain very nicely
@MissSkello
3 жыл бұрын
thank you.. glad it helped :)
@KBRCREATION
Жыл бұрын
Can u please explain the cytological proof of crossing over in drosophila ?
@MissSkello
11 ай бұрын
Sure!
@MissSkello
11 ай бұрын
kzitem.info/news/bejne/ko9umoB-r6yDZYY Please find the video through the link!
@ismartharinekaa4670
2 жыл бұрын
Very good explanation, it helped a lot
@amals3645
10 ай бұрын
Thank you so much
@pandapanda9323
2 жыл бұрын
Tq it is very helpful can uh pls provide the note
@ponmanickam
2 жыл бұрын
Thank you very much madam.
@MissSkello
Жыл бұрын
So nice of you
@The_Variety_Vault0
Жыл бұрын
Thanku very much mam
@naveednaqvi8078
3 жыл бұрын
Thanks for guide My question here is shall the baby boy be affected always i. e in each progeny
@MissSkello
3 жыл бұрын
yes
@bhawanasharma4580
3 жыл бұрын
Best
@rupalichakraborty4814
Жыл бұрын
Thank
@Amar45611
3 жыл бұрын
Thank you so much for this video ma'am❤
@agrigpb
Жыл бұрын
Nice one....
@MissSkello
Жыл бұрын
Thanks 😊
@tahmeedsdaily6545
2 жыл бұрын
Helpful
@kritibajaj8780
3 жыл бұрын
V. Good 👍🌸
@sachinp6966
2 жыл бұрын
Nice explaination Mam👍👍👍
@MissSkello
Жыл бұрын
It's my pleasure
@rojalinsubudhi954
10 ай бұрын
Nice mam
@soha99mohammad
3 жыл бұрын
What is the technique that used to detect the mRNA? I know that is northern technique but my question is why we don't converts the mRNA to cDNA then used southern technique?? I hope to answer me! 💙🌷
@MissSkello
3 жыл бұрын
but why add the extra step when you can directly detect RNA in the sample using northern technique?
@soha99mohammad
3 жыл бұрын
Ok, thank you 💙💙
@pujithaakkala2323
Жыл бұрын
Thank you so much mam
@MissSkello
Жыл бұрын
Most welcome 😊
@arshorts7760
3 жыл бұрын
Ma'am I'm a College Student zoology hons . Its😍very helpful 🥰 Thnk u so much! Mam can u Explain why The Male die?
@MissSkello
3 жыл бұрын
Thank you The male dies because the mutation we are looking for is sex linked, lethal and recessive. As in male, only one copy of allele is present on X, the recessive allele will express itself and cause death.
@bioxploredia750
3 жыл бұрын
Nice
@aniketdas7803
Жыл бұрын
Very helpful , thank you ❤
@MissSkello
Жыл бұрын
Glad it was helpful!
@barsharanibehera9955
3 ай бұрын
Too muchslow... Explainingg.. Soo idl Rest all.. Awesome ma'am
@MissSkello
3 ай бұрын
I used to talk fast but some couldn't follow that 😅. you can try 1.5x
@barsharanibehera9955
3 ай бұрын
@@MissSkello already tried.. Thank you ma'am 🙏🏻
@naveednaqvi8078
3 жыл бұрын
Plz tell the precautions
@MissSkello
3 жыл бұрын
basic experimental precautions like using the mutagen properly and carefully so as to avoid exposure. count the progeny carefully. handle your test subject with care identify the sexes properly
@anshuyadav2303
3 жыл бұрын
Thank u mam 🙏
@gowthamhn5112
2 жыл бұрын
In the second generation the female genotype taken is X'ClB , if the X' (Which is irradiated with x rays) had mutation then the X'ClB progeny won't survive itself ,then why it is further multiplied(practically not possible) for test the mutation in its progeny?
@MissSkello
2 жыл бұрын
X' and ClB both contain a lethal gene but they're not present on the same loci. This doesn't form a homozygous condition and the female can survive because both the mutations are recessive
@mariemalchereck6976
3 жыл бұрын
Thank you very much for your video! This whole method only shows lethal mutations, right? So if the x-rays causes mutations that are not lethal, there is no way to detect them?
@MissSkello
3 жыл бұрын
most welcome! yes this is just for lethal mutations. this can't detect mutation if its not lethal. there are other methods available for detecting those.
@mariemalchereck6976
3 жыл бұрын
@@MissSkello thank you very much :) your work is highly appreciated!
@MissSkello
3 жыл бұрын
thank you :) glad i could help
@s.j5271
10 ай бұрын
@@MissSkello10:04
@kanikshagarg1728
2 жыл бұрын
Mam koi book refer kr dijiye iss topic ke liye... Kis book me diya hua h ye topic
@MissSkello
2 жыл бұрын
i refered to different sources on google. i didn't find a book for this topic
@TAEKOOKISM
4 жыл бұрын
In the F2 generation why the X+X' female became normal instead of being mutated by X' ?
@MissSkello
4 жыл бұрын
X' is a sex linked *recessive* lethal mutation. Finding if that mutation is present or not is the aim of this process.
@akshatahattiholi8016
4 жыл бұрын
Mam,In F2 the second progeny's explaination...I didn't get it...2:1 by seeing this ratio how will come to know that whether the male is muatated or not?
@MissSkello
4 жыл бұрын
If male is mutated, it will die because the mutation we are looking for is lethal. If the mutation is not present, the male will survive. That's how ratio will help in seeing whether male lived or died.
@tarunkumarpalli7580
3 жыл бұрын
Wht happend if the mutation by x rays on previous wild male is induced exactly the same region as in lethal gene of clb, then the combination of clb and x'(mutated) will die, so there will be one female and one male in the first region Or The lethal gene in clb is just constructed artificially and then inserted to build clb?
@MissSkello
3 жыл бұрын
clb is constructed artificially
@achyutgogoi4724
10 ай бұрын
I dont understand the last X"Y condition
@saurishsengupta844
2 жыл бұрын
Thanks a lot for this video ma'am 🙏🙏. But I had 2 questions regarding this - 1. An organism must have an XX genotype in order to be a female right ? How can then the Drosophila having X (CLB) genotype be considered a female ? 2. Do the X - rays induce mutations only on the X chromosome ? If yes, then how is it that the rays are so specific ? If no, then why is it that the males having the XY genotype don't die ? Would be grateful if you could spare some of your precious time to clarify these doubts of mine 🙏🙏❤❤.
@narenderbhati3661
2 жыл бұрын
Ya same question , shurwat m hi die ho jata male agr mutation hui h to
@MissSkello
Жыл бұрын
hi! sorry for the late response 1. the chromosome clb is still an X chromosome. the Clb just denotes the type of mutation on it. so therefore the organism is still female. I've explained the meaning of clb in detail at the beginning.
@MissSkello
Жыл бұрын
2. when we study mutations, we rely a lot on "chance". the rays can mutate Y but because of it's small size, chances are low. X is highly mutable. also, mutations on Y rarely survive. the XY that we screen during step 1 don't die because of this. we assume Y is not mutated here coz survival is questionable. so we check the X.
@saurishsengupta844
Жыл бұрын
@@MissSkello thank you so much for the answer ma'am 🙏🙏. But by the time you have replied to this question, I am preparing for the last of my 6th semester exams (this topic was in 5th sem) 😊😊. No issues at all, since I understand you have comments flooding in from all the videos on your channel. Hope that this reply benefits someone else having the same doubts. Best wishes for your channel ma'am 👍👍. May you keep spreading the light of knowledge with your videos to more and more students 😊😊❤❤🙏🙏
@sampurnadasgupta8328
3 жыл бұрын
in the first cross, if there had been a mutation due to irradiation then would there be clb+X' as a progeny?
@MissSkello
3 жыл бұрын
yes
@ambarishbhuyan6727
2 жыл бұрын
Why in the first cross the XX chromosome containing the ClB and irradiated X does not dies?
@MissSkello
2 жыл бұрын
because the mutation that we're studying is recessive. if xx condition is present, the recessive gene can't express itself
@gauravmhapsekar9542
3 жыл бұрын
how did you determined progenys sex ratio? thanks
@MissSkello
2 жыл бұрын
by observing it morphologically male and female drosophila look different
@mollyrulze7
4 жыл бұрын
Good going girl 👍
@MissSkello
4 жыл бұрын
thank you
@tahmeedsdaily6545
2 жыл бұрын
I have problem with x'y, why it will die! Please let me know in details
@MissSkello
Жыл бұрын
we are checking for lethal mutations. so if X is mutated, it will lead to death of the organism. that's the principle behind the test
@dhanushnagendra
7 ай бұрын
@@MissSkello then why X'Y (parental) which was irradiated initially won't die
@shadowman3843
3 жыл бұрын
(CLB X'), Is it male or female??? Honorable sister, I want to know as soon as possible.
@MissSkello
3 жыл бұрын
its a female since the ClB chromosome was an X chromosome.
@shadowman3843
3 жыл бұрын
@@MissSkello Thank you so much, Sister 💕 May Almighty bless you and go ahead with full of confidence ☺️
@poonamthakur9600
2 жыл бұрын
Mam I got all things but what is +nt and -nt at the last male ???
@rohinimishra3276
Жыл бұрын
+NT STANDS FOR PRESENT AND -NT STANDS FOR ABSENT
@swathiamara1924
2 жыл бұрын
Mam chromosomal abbreation mam both structural chromosomal abbreation in detail mam please for BSC student
@tulsijoshi1046
3 жыл бұрын
Is x' clb combination progeny going die in F1 generation because one x has mutated genes and other x chromosome has Lethal alles?
@MissSkello
2 жыл бұрын
but they aren't necessarily on the same locus and therefore aren't present in homozygous condition. therefore the clb female won't die
@kidslaugh432
3 жыл бұрын
Mam plz make a video of attached X chromosome technique and Russell's test for Dominant Lethals in mice..Thank you 😊
@MissSkello
3 жыл бұрын
sure.. look out for the notifications! ☺️
@MissSkello
3 жыл бұрын
Attached X method is now available on my channel in the same playlist. ☺️
@kidslaugh432
3 жыл бұрын
@@MissSkello thank you mam😊
@vigneshs5286
3 жыл бұрын
Tq tq tq
@swamineesanap5695
2 жыл бұрын
Mam cn u tell m reference book for this topic..plz reply m faat
@swamineesanap5695
2 жыл бұрын
Reply m fast mam
@MissSkello
2 жыл бұрын
you can refer to klug and cummings for genetics and mol bio. it was good explanations. or you can read articles and papers online. that's where i refered for this video
@swamineesanap5695
2 жыл бұрын
@@MissSkello okey mam...I wl read those books..if I get..thnk you mam
@naveednaqvi8078
3 жыл бұрын
Does this mutation leads to congenital myopathy.
@MissSkello
3 жыл бұрын
no because its not a lethal mutant
@MissSkello
3 жыл бұрын
i mean it doesn't lead to death of a zygote
@naveednaqvi8078
3 жыл бұрын
My son died at the age of 9 to10 months.congenital myopathy and brochul pneumonia diagnosed What I should expect in next progeny plz
@naveednaqvi8078
3 жыл бұрын
Plz tell the precautions for congenital myopathy and test if any
@MissSkello
3 жыл бұрын
I'm sorry but I'm not a medical professional. you should contact genetists or doctors. they'll guide you
@aathirareghu4093
3 жыл бұрын
But i still didn't get why the males are treated with x Ray?
@MissSkello
3 жыл бұрын
basically we have to understand if xray is a mutagen, right? so we will have to treat the organism with it. we treat males because they are hemizygous and any sex linked mutation (what we have to recognise here) will be expressed for sure. with females even if mutation occurs, it is recessive and hence, won't be expressed and we won't know if xray did something or not. hope that clears it up
@aathirareghu4093
3 жыл бұрын
@@MissSkello thank you mam
@RomyBudhwar
4 жыл бұрын
👍👍
@kshetramohannaik4766
3 жыл бұрын
🙏🙏🙏🙏🙏
@vasundhararaina6849
10 ай бұрын
Not helpful. It’s very confusing
@MissSkello
9 ай бұрын
it is a little advanced. try to read from some other source as well. maybe then it'll be clearer🙂
@mfbvlog
4 жыл бұрын
But was confused in stay alive stay dead
@MissSkello
4 жыл бұрын
hi! I did not get your doubt ..if you can rephrase your question exactly, I'd love to help you out
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