With demand for faster, more accurate diagnostics, medical and life sciences pros require analytical insights and tools that are smarter, simpler and more robust. In this demo, we use high-sensitivity surfaced-enhanced Raman spectroscopy (SERS) for rapid detection of a COVID-19 antibody. Learn how SERS can be implemented into portable systems developed by manufacturers for virus detection and testing, when to use standard substrates or liquid SERS, and how a few simple tips will ensure best results.
Overview of COVID-19 antibody detection options
We have four tools to actively fight the coronavirus pandemic, including social distancing, vaccines, treatments and detection. Ocean Insight looks at the detection aspect in this video - namely, identification of COVID-19 antibodies using high-sensitivity surfaced-enhanced Raman spectroscopy. Detection includes exposure of the virus itself - for individuals who are currently affected - and antibodies to let us know where the virus has been.
Benefits of SERS for rapid detection of COVID-19 antibodies
Raman, a laser interrogation technique, helps speed up the detection of COVID-19 antibodies. Using the Ocean Insight QE Pro Raman+ high-performance spectrometer, we help customers push the limits of spectral detection.
Depressions and amplifications of peaks in this demo are enhanced with SNV View transformed intensity reading. Ocean Insight's featured software makes COVID-19 antibody detection easy with meaningful measurements and unique peak activities, even at trace levels.
Ocean Insight uses LSERS or liquid-state SERS substrates that remain stable for a year or more. Another significant advantage of using LSERS is repeatability for the analyte's concentration and quantification, allowing you to quantify findings with much higher accuracy and confidence. Water-based solutions will never burn, even at maximum laser power.
Overview of our demo results
When we place 10 microliters of our COVID-19 antibody into the LSERS, we get a fresh reading yielding a quick snapshot of the results. In sum, it's incredibly easy to apply some sample into an LSERS measurement before taking an image and evaluating the results.
We recommend taking an initial background snapshot, finding that 'Goldilocks Zone' for laser power, considering same substrate multiuse limits, using no more than 15 microliters of analyte and finding the right combination of metal (gold or silver SERS substrate), wavelength and analyte to get the most out of your Raman signals.
About Ocean Insight
Ocean Insight offers application consultancy, new technologies, manufacturing expertise, custom system design and a global service and support network for businesses looking for custom solutions.
Timestamps:
[0:05] Detecting COVID-19 antibodies using SERS Raman detection
[0:45] Overview of COVID-19 antibody detection options
[1:19] What is a Raman shift?
[1:55] How well the SERS setup detects antibody samples from SARS-CoV proteins
[2:02] Hardware used for high-sensitivity surfaced-enhanced Raman spectroscopy
[4:44] How emissions appear in Wizard Software
[5:13] CleanPeaks algorithm explained
[5:42] SNV View transformed intensity reading
[6:17] Standard Normal Variate (SNV) Transform formula and advantages
[7:09] OceanView enables you to take convenient snapshots of what you're looking at throughout the detection process
[7:59] New activities exposed by applied COVID-19 antibodies in SNV View
[9:37] This form factor uses gold nanoparticles (GNPs) embedded into a proprietary quartz matrix - we also offer it in silver
[10:03] LSERS explanation and benefits
[12:25] Snapshot of liquid SERS measurement sample - fresh reading software results
[12:45] SERS and LSERS additional tips and tricks
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