In this video I have practically shown that how one can find the sequence of the gene of interest using NCBI www.ncbi.nlm.nih.gov and how to find the transcription start site (TSS) and how to compare and contrast it with www.ensembl.org/index.html Then I have shown how to sign up for your account on www.benchling.com How to import your gene to design sgRNA(s). The last part of the video shows that how to check your designed sgRNA(s) for On- and Off- targets using NCBI BLAST. Next video will be based www.addgene.org Keep watching and please ask questions in comments.
- 4 жыл бұрын
Designing of sgRNA Using Benchling- CRISPR Cas9 Gene Editing Technology- Design Your Project 4-B
- Рет қаралды 6,852
Пікірлер: 26