Presented By: Steven Wang, PhD
Speaker Biography: Dr. Steven Wang is an engineering manager for microfluidics and consumables at Bio-Rad Laboratories. Dr. Wang completed his bachelor’s in chemical engineering at Texas A&M University. He then went to the University of Michigan to do his MSE and PhD in chemical engineering. After completing his PhD in 2017, Dr. Wang worked for several years for the Air Force Research Lab at Edwards Air Force Base doing research in developing propellants in microfluidic devices. In 2019, Dr. Wang started working as a microfluidic engineer at Celsee Inc., a start-up company focused on developing single-cell systems based in Ann Arbor, MI. After Celsee got acquired by Bio-Rad Laboratories, Dr. Wang transitioned into the Digital Biology Group at Bio-Rad Laboratories, continuing to focus on single-cell system development. Dr. Wang recently moved into managing the microfluidics team with the Digital Biology Group, expanding his knowledge and expertise into digital droplet PCR.
Webinar: Droplet Microfluidics in PCR: Technology and Applications
Webinar Abstract: Digital Droplet PCR (ddPCR) is a method for performing digital PCR use microfluidic water-oil emulsion technology. By utilizing microfluidics and fractionating DNA samples into nanoliter droplets , ddPCR enables absolute quantification of DNA. In comparison to traditional qPCR, ddPCR has demonstrated improved precision and accuracy, better sensitivity, and increased signal-to-noise ratio. ddPCR also allows for traditional qPCR techniques including different probe chemistries and multiplexing. ddPCR has enabled and improved many applications including NIPT, pathogen detection, food safety analysis, and liquid biopsy.
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