Hi Hyeju, i really enjoyed your vid so much !!!! i got some knowledge from cell culture. thankssss
@user-dr1cm5bj2x
9 ай бұрын
Hi Hyeju. I'm glad I found your video! To find the best RNA concentration quality, we must do cell seeding optimization. Thus, when doing cell seeding optimization in a well plate, is it better to do it with treatment or without treatment?
@lola-mn2kw
4 ай бұрын
Thanks for efforts,please keep going, more videos with english subtitles.❤❤❤❤❤
@PurpleNoodles
Жыл бұрын
Your videos are sooo cute!
@hyejulee7072
11 ай бұрын
Thank you sooooo much !!!
@MrWTFYOUWANT
Жыл бұрын
Great video! What kind of experiments are you doing?
@hyejulee7072
11 ай бұрын
Now days, I'm working on muscle atrophy using C2C12 cell, which mouse myoblast cell line !
@aakifinayat8234
10 күн бұрын
i am using HEK293T cells,i washee my cells,and then trypsinized it centrifuged it and then added 1 ml trizol to the tube,and stored it on -80°c....i will perform further steps later....now someone tell me is this procedure correct or not? as i am a beginner
@mahsunkarimi2479
11 ай бұрын
I add TRIzol to cells in 24- well plate to extract protein and RNA and I have 4 repeat for each treatment, but unfortunately the amount of RNA extracted from each well is very different from next well. Do you have any idea what I could do to resolve that issue?
@hyejulee7072
11 ай бұрын
The amount of RNA may vary depending on the number of cells seeding the cell. Next time, How about seeding on a 12well plate rather than a 24well plate?
@meghanaharidas7859
Жыл бұрын
Home made TRIzol? BTW thanks for the video.....
@hyejulee7072
11 ай бұрын
Our lab makes TRIzol and uses it.
@romitkumarsubba4138
Жыл бұрын
what confluency of cells is required for rna extraction?
@hyejulee7072
11 ай бұрын
It usually depends on the cell type and plate ! I think more than 80%.
@anjhere
Жыл бұрын
isopropanol centrifugaton 60 min?
@hyejulee7072
11 ай бұрын
This process is necessary to centrifuge RNA pellets !
@anjhere
11 ай бұрын
@@hyejulee7072 but doesn’t all the unwanted salts also be precipitated if you keep the RNA in isopropanol overnight? I have heard it’s better to keep max 30 mins in ice while isopropanol precipitation of RNA.
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