Last footage of my brain cells. 2019 (Not Colourised)
@M60T
3 жыл бұрын
yes... you are seeing yourself right now amazing isnt it?
@feliperamedeiros
2 жыл бұрын
what were they thinking?
@binh3308
6 жыл бұрын
Neurons look like people to us
@qondonyon
Жыл бұрын
Very Epic Very Relatable
@alcikelly2496
Жыл бұрын
What are those two dark spots seen underneath the neuronal cells?
@wolfgangouille
Жыл бұрын
I'm not sure I understand what you mean. If you see a dark shadow somewhere that is not moving it is probably some kind of dust on the other side of the slide.
@MultiNeurons
11 жыл бұрын
Wonderful video!!!
@estranhanatureza3978
2 жыл бұрын
Mr wolf. if you do reply, would like a clue about nutrients in plate
@wolfgangouille
2 жыл бұрын
Neurobasal with B27 and a little bit of serum.
@Siliconsbrain
6 ай бұрын
Amazing
@ojofars1442
2 жыл бұрын
I have so many questions...
@wolfgangouille
2 жыл бұрын
You can always ask.
@user-tk9cb2dl6l
3 жыл бұрын
I wanted to ask you , if you believe there is any substantial difference between neurons death in vitro caused by lack of culture vial and neurons death in vivo caused by lack of blood flow following heart arrest . Do you think both neurons show a similar dying pattern as their nourishment is deprived ? I know of the basics , loss of potential , loss of the axon , membrane disintegration , nucleus loss as a general feature of necrosis . But do you think the 2 situations in vivo ed in vitro are comparable ? Can you think of any differences ? And lastly the most interesting question in my idea . Despite the common belief that irreversible brain damage and brain death occurs after 5-7 minutes of absence of blood flow , I would like to ask you , if you believe applying high voltage on the primary motor area post- mortem , let’s say after 30 mins from absence of blood flow to the brain , you could still elicit a motor response of any type . I read about post mortem stimulation directly on the muscle , on the peripheral nerve , but I was wondering if the descending pyramidal neurons would be completely destroyed and the whole brain irresponsive to electrical or chemical stimulation .
@wolfgangouille
3 жыл бұрын
In my videos, neurons are dying from drying of the medium actually. Dying from lack of nutrients or inhability to remove waste is probably a slower process in culture. The problem with neurons is that they don't have the ability to use fermentation like the other cells (especially the muscles) and therefore require oxygen for respiration. So the effect of the blood flow is more an oxygenation issue, but without oxygen, the cells can not make ATP and maintain ionic flux required for their survival. There might be other details I ignore but I think that is why you really can't hope brain cells to remain alive for so long after cardiac arrest. I wonder if astrocytes can help neurons survive in the brain, they definitely do in culture.
@user-tk9cb2dl6l
3 жыл бұрын
wolfgangouille yeah I can’t explain in an evolutive manner why those genes (the ones that allow storage and usage of glycogen for istance) aren’t expressed in neurons . It seems like absence of blood flow causes braindeath within 5 to 10 minutes , a patient I checked on during training would exhibit fixed dilated pupils after around 4 minutes of cardiac arrest , as if the neurons in the brainstem were already not functional . But I was really wondering , and here would come your great help, what meaning I should give to this situation . My impression is that in heart arrest the brain just turns quickly into a non usable matter that you could simply take and throw into the junk , since everything there is turns rather quickly into dead / not functional tissue. But my curiosity came from the fact first experiments involving electricity even hours after death would cause muscle contractions (think of frankeinstein) . Is it because electricity was applied on the muscle which stays vital for a longer time ? Or is it possible that you could elicite some type of electrical (or chemical response) in a corpse brain , even hours after loss of perfusion ?
@user-tk9cb2dl6l
3 жыл бұрын
wolfgangouille consider that most fresh brains I saw in autopsies , even up to 5-6 hours after death , looked grossly normal , minimally edematous , so I wonder if there was the possibility that some microscopic organization was also preserved and some neural circuits still intact . Sadly it isn’t ethical or even legal in my country to do tests in this matter . As also Im not sure a clinical diagnosis of brain death means every cell is dead .
@user-tk9cb2dl6l
3 жыл бұрын
wolfgangouille AH and yet one last question if you were so kind to reply to this , I had the chance to observe a lot of tissue samples (common practice in pathology to diagnose cancer , and other conditions) , yet there is obviously no post mortem changes since the tissue has it is taken out is immediately put into formaline . Do you if I took a brain slide after 5-6 hours , even tho it looks grossly unremarkable at autopsy , and put into formaline and colour it , I would observe a lot of post mortem changes ? (Necrotic features)
@wolfgangouille
3 жыл бұрын
@@user-tk9cb2dl6l One reason why neurons do not use fermentation is because fermentation creates lactic acid or ethanol and neurons are very sensitive to it. Some muscle cells stay alive for days after death actually, and no doubt this is possible because of fermentation which allows them to make some ATP, and therefore maintain the membrane potential somehow via the Sodium and Potassium pump ATPase.
@marshmallowbudgie
Жыл бұрын
"let's have an idea"
@trillioncrowns
2 жыл бұрын
Humanity is replicating the brain! We need to reach 100 billion people
@wolfgangouille
2 жыл бұрын
86 billion.
@trillioncrowns
2 жыл бұрын
@@wolfgangouille i dont drink alcohol and micro dose mushrooms! 100 billion
@sapiranimations
8 жыл бұрын
Amazing! what magnification is that?
@wolfgangouille
8 жыл бұрын
+sapiranimations I used 20x if I remember well but I made a mosaic from many fields. The body of a neuron is about 20 microns in diameter.
@sapiranimations
8 жыл бұрын
+wolfgangouille That's really awesome, Are you working in a professional lab or did you manage somehow to grow them at home?
@wolfgangouille
8 жыл бұрын
I did this in a lab. Cultivating mammalian cells would be very difficult to do at home. And expensive.
@eltelontheatercompany9568
9 жыл бұрын
Hello, I am Theater student and I would like to use this video in one performance that I am planning, which will be a connection between science and theatre. so, I would like to know if would be ok to use this video at some point of the performance? :)
@wolfgangouille
9 жыл бұрын
Sure. Just add my name in the credits :)
@DEADmetal3
7 жыл бұрын
Hey, i have a question which is important in my decision making for cryopreservation. I will not go into details here but it's important. Do neurons lose their synapses when they die in a neuron system (brain)? How soon do they die?
@wolfgangouille
7 жыл бұрын
What do you mean? Synapses are parts of neurons. When neurons die, their synapses stop working immediatly and evenually disappear.
@DEADmetal3
7 жыл бұрын
Your reply is just in time. Today i have to decide if i want someone to be cryopreserved. It's a tough case and there are some very negative factors. Do you mean, when neurons die (specifically, in a brain of a dead body, after death), they lose their synapses eventually? If so, do their synapses disappear without any trace / scars / leftovers? If you have any expertise on that... i unerstand these are not simple questions.
@wolfgangouille
7 жыл бұрын
Žilvinas Deveika dont do it, it is a scam.
@adriandelcan35
2 жыл бұрын
Hello! I'm a film student in Southern California and was wondering if I could use this clip in my short film?
@wolfgangouille
2 жыл бұрын
Sure, just put the name of my channel in the credits. Also feel free to share your short film when it's done !
@adriandelcan35
2 жыл бұрын
@@wolfgangouille Of course! How should I contact you?
@wolfgangouille
2 жыл бұрын
@@adriandelcan35 my gmail address is same as my channel's name.
@josecarrascopujante
2 жыл бұрын
Hi Wolfgang, I could not find this question in the comments: which was the microscopy technique you used to create this video? Was it phase-contrast? I need record two-week long time lapses of neuronal development (I am a PhD student in neuroscience) and want them to look like they do in this video. Could you please also add the brand and model of the microscope/camera you used?. Your help in this matter would be very much appreciated.
@wolfgangouille
2 жыл бұрын
It was DIC not phase contrast I believe. I used a Leica SP5 confocal microscope but you could use any microscope. Autofocus is a must for time lapse. I also tried Nikon microscopes, they have a "perfect focus system" which measures objective-specimen distance and this got me nice movies.
@josecarrascopujante
2 жыл бұрын
@@wolfgangouille Thanks a million for your reply. Would you mind expanding a bit more on the settings you used to perform DIC imaging in that confocal microscope? Actually we have been thinking of using a very sophisticated, ridiculously expensive Carl Zeiss confocal microscope we co-own with other labs in our University, but for this I would need a bit more of detail on what settings to use in it. Thanks in advance.
@wolfgangouille
2 жыл бұрын
@@josecarrascopujante Since it was 8 years ago I do not remember the details. I was taking a picture every 5 minutes I think, and it was a scanning line not a spinning disk confocal, but beyond this I wouldn't know. I think it was a 10X objective.
@josecarrascopujante
2 жыл бұрын
Hi Wolfgang, could you please elaborate or paste a link to a webpage explaining the difference between line-scanning and spinning disk confocal technologies? That way I will be able to check what type is ours and figure out how that could affect our experiments. Thanks a lot in advance, this shows KZitem comments can actually be useful and civilized 🤙.
@wolfgangouille
2 жыл бұрын
@@josecarrascopujante A confocal isn't even necessary, especially in phase contrast or DIC. You could do amazing time-lapses with a regular microscope. What you should focus on : your objective, your camera, ability to focus and to keep neurons alive.
@nikolaifriendly7977
4 ай бұрын
This footage is not of brain neurons. Dendrides and dendritic branches of human brain look like tree roots. On this footage dendrites are missing.
@wolfgangouille
4 ай бұрын
They are, in fact, neurons. I literally took them from a mouse brain myself. There are many types of neurons in the brain, not all of them have a typical pyramidal neuron dendritic tree, and this is a 2D culture so their morphology is also different.
@yoskahernandez9889
3 жыл бұрын
Hi! can I use your video on my youtube channel? I'll credit you :)
@yoskahernandez9889
3 жыл бұрын
I'd like to use just few seconds
@wolfgangouille
3 жыл бұрын
@@yoskahernandez9889 Sure.
@amysterling
10 жыл бұрын
Whoa! Did you make this?
@wolfgangouille
10 жыл бұрын
Yes. But they started to die at the end, medium was drying out :(
@Laserblade
10 жыл бұрын
Wow! To see the neurons reaching - stretching to make connections. Like they sense the proximity of neighboring neurons. Wonder what the timespan of the clip is?
@wolfgangouille
9 жыл бұрын
***** maybe 12 hours. I don't remember.
@wolfgangouille
9 жыл бұрын
Amy Robinson Oh I just saw you are involved in the EyeWire project ! I used to play when my time-lapses were running. Funny !
@amysterling
9 жыл бұрын
Seriously? AWESOME! Yea I'm Creative Director over at EyeWire! Come say hey sometime!
@thelawnmowerman599
3 жыл бұрын
My brain loves to look in the mirror. Replay
@dreamybullx1
6 жыл бұрын
Disguisting but amazing to see what is going on in a brain
@exolocke2463
Жыл бұрын
Okay and?
@vinishvijayan6661
Жыл бұрын
Such an irritating bgm
@wolfgangouille
Жыл бұрын
It is a classic, used in the opening scene of one of the greatest movies of all times, 2001 a space odyssey. You can always mute or put a different music on your own videos.
Пікірлер: 71