Very helpful! I was struggling to find information on how to clone genes using homologous recombination, but this explains it very well!
@triptimisra7810
Жыл бұрын
How do we add the plasmid sequence ??? Antibiotic cassette
@suparnachakraborty4480
4 жыл бұрын
Helpful video indeed. But eager to know the maximum length which can be deleted by this lamda red technique. If I want to delete a larger segment of the genome (more than 10 kbp) , will this work?
@lilpiggie2167
4 жыл бұрын
Yes, that should be doable, but may be more challenging. I would recommend constructing 500bp homology arms rather than just the 40bp oligo arms.
@rakeshchaudhary2246
5 жыл бұрын
Hello Sir, I need your help. I have to perform gene deletion in bacteria by homologous recombination, using pkd46,pkd3 and pcp20 plasmid... Please help me out of this....
@Krishna-ut8jl
6 жыл бұрын
A bit late here but this is very well explained. Great job!
@philippedesbiens-fortin8185
3 жыл бұрын
It is well done, but it only occurs during bacterial replication which is important to highlight
@CarlosMartinez-jn2cf
3 жыл бұрын
Amazingly done!
@adeebqunash3985
2 жыл бұрын
I would honestly says that his makes it seem more complicated than it is.
@mehrdadnorouzi9562
4 жыл бұрын
do not just read your handwritings. try to explain. if you tell us just by some stuffs in this speed , we cannot still get what you say
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