If the variety to be used as a male happens to be in flower at the same time as the female (in grape breeding lingo, they "nick"), then this step can be easily accomplished by collecting a few of the flowering clusters for pollen. However, differences in timing require other approaches. If the male is significantly earlier, it may become necessary to collect and store pollen.
Before the flowers open, each one is covered by a cap, short for calyptra. Though this cap eventually dries and comes off on its own, it must be removed a day or two before the cross is to be made to prevent self-pollination with perfect-flowered varieties.
Crossing is often simply a matter of brushing the pollen onto stigmas of the emasculated cluster. If both parents are flowering simultaneously, the pollen-bearing cluster can be gently brushed against the emasculated female. However, if pollen was collected earlier and stored, application with a soft paint brush is recommended. Care must be taken to sterilize the brush (and your hands) with 70% ethanol between pollinations.
After pollen has been thoroughly applied to a cluster, taking care to brush every flower, the paper bag is replaced over the cluster, both to prevent fertilization by other pollen sources, and to protect the developing fruit from birds and the elements. The bags also help to identify the cluster locations at harvest time, at which time the seeds are removed from the individual berries.
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