Agarose gel electrophoresis is used to resolve DNA fragments on the basis of their molecular weight. Smaller fragments migrate faster than larger ones; the distance migrated on the gel varies inversely with the logarithm of the molecular weight. The size of fragments can therefore be determined by calibrating the gel, using known size standards, and comparing the distance the unknown fragment has migrated. This technique can be used to resolve complex DNAs (i.e., genomic DNA)
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