thank you in advance for your explanation. I don't have experience working GC-MS .after watching in the video I have a question . output of GC-Ms is chromatogram that shows abundance versus M/Z that molecular weight is calculated . how to calculate concentration of compound?
@shirwansany8706
2 жыл бұрын
Hello Shiva, thank you for the question. I have uploaded a video on determining the concentration of compounds via the GCMS here: kzitem.info/news/bejne/tX2n1YSCpIGfdGk Hope it answers your question. Thank you for watching this video.
@giuseppezedde4553
2 жыл бұрын
Thanks so much for your explanation. I have a question for you. Is the mass spectrum sufficient to identify a compound or is it also necessary to have the IR, NMR and UV spectra? Thank you
@shirwansany8706
2 жыл бұрын
Hi, It depends on the aim of the study as follows: 1. Shall you need to identify a new compound, mass spectrum, IR and NMR are needed. 2. Shall you need to confirm the presence of an already known compound, get the mass spectrum and verify it with the compound standard are recommended. Thank you for watching the video.
@giuseppezedde4553
2 жыл бұрын
@@shirwansany8706 I thank you so much!
@MahnoorUsman-je7te
Ай бұрын
I have a question that how can we get this gc ms library???? and which software are you using??? Thank you in advance.
@scientiainquisitor5391
Жыл бұрын
Hi there are multiple compounds detected per peak So which one will be the compound corresponding to the peak. Are all the compounds are said to be present in the single peak (whichever is detected).
@rahanjdas5572
2 ай бұрын
hello did you get the ans for this question???? i am also confused
@rajendraregmi5407
Ай бұрын
I have the same question as well.
@scientiainquisitor5391
27 күн бұрын
@@rahanjdas5572 nope
@rahanjdas5572
27 күн бұрын
@@scientiainquisitor5391 thanks for letting me know
@iqbali8944
3 ай бұрын
Can we identify compound on the basis of retention time ?
@shirwansany8706
3 ай бұрын
With GC/MS, you can identify a compound with (2) Mass spectra of the compound and (2) retention time.
@nazishakhtar2
Жыл бұрын
Wslam sir! I have to compare compounds in plants of 4 different groups, is GCMS can interpret the different concentrations of the same compound in different groups of plant
@shirwansany8706
Жыл бұрын
Salam, GCMS can identify the volatile compounds in the plant. Here is the strategy: 1. Analyze the plant extracts via GCMS. Determine the detected compounds, qualitatively. 2. Based on the detected compounds, purchase the compound standards. 3. Analyze the standards via GCMS and set up a calibration curve for each standard. 4. Analyze the plant extracts. Then quantify each compound using the calibration curve.
@akiamini4006
Жыл бұрын
Very nice
@shawonislam2724
10 ай бұрын
Very helpful
@shawonislam2724
10 ай бұрын
Wow❤
@TheMeemkim
3 жыл бұрын
Thanks!
@TheMeemkim
2 жыл бұрын
Walaikum Salam :-)
@samsoonmaharifa6687
3 жыл бұрын
Walaikum mussalam sir The library is showing three hits What we have to consider, only the first hit alone or all the hits Because it shows different compound name If it is same we can take that without doubting, hit is showing three different compounds for a particular peak Please help me in this
@shirwansany8706
3 жыл бұрын
Hi Samsoon. In my case, if the extract is crude, I don't consider the hits as the correct result. Then, I submit the sample to another detection method to identify the compound. If the extract is the pure one, we could possibly accept the first hit; however, please bear in mind, the FTIR only identifies the functional group, not the actual chemical compound.
@rajendraregmi5407
Ай бұрын
When you select some peak so many compounds were shown, how to conform the given peak is of respective compound
@shirwansany
Ай бұрын
There are a few ways to confirm. 1. Confirm with the standard of the compounds. Run a standard of the compound, record and compare the mass spectra. 2. Based on the mass spectra of the compound, confirm the mass of ion fragments
@rajendraregmi5407
Ай бұрын
@@shirwansany I mean when you click some peak many mass spectra is shown? I need to identify Z-7-dodecyl acetate ? how can i do that? Anyways thanks for your video.
@syarifahyantiastryna104
10 ай бұрын
Belajar lagi❤❤
@md.mahmudulhasan5905
4 ай бұрын
from Bangladesh
@pentagc672
2 жыл бұрын
which is this application name where we can buy this app, pls share this link.
@shirwansany8706
2 жыл бұрын
Hello, this is Enhanced Data Analysis software from Agilent, which came together when we purchased the GCMS.
@DurayStuffCrafts
2 жыл бұрын
How to calculate percentage by using giving data
@woba7166
2 жыл бұрын
You need to calibrate with known concentrations of compounds in question.
@franklindebruin2074
2 жыл бұрын
Hi sir great video where can I get the software from I'm from south Africa thank you can you assist with software
@shirwansany8706
2 жыл бұрын
Hello Franklin, I am using the Agilent OpenLab CDS Data Analysis software. It came with the HPLC instrument.
@pentagc672
2 жыл бұрын
@@shirwansany8706 Agilent open lab software has using complex sir, i used above that software using friendly.
@technologywatch7619
Жыл бұрын
Can show how to open sample data firtst/
@haaniaishaq4966
Жыл бұрын
I need software plz share ur mail id
@shirwansany8706
Жыл бұрын
Hello, this is Enhanced Data Analysis software from Agilent, which came together when we purchased the GCMS.
Пікірлер: 39