Gary, you have no idea how ecstatic and thrilled I was when I saw you put the beginning and the results. Some you tubers would split videos and it’s better personally when we can see it in the same video. Stellar job with these new breeding cultures
@johnathanfaircloth9639
Жыл бұрын
It's good to see that the only ones messed up had imperfections in their Jars.
@jerodyoumans6002
Жыл бұрын
One of the better videos in/or on mycology before, after and in betweens, with examples of mycelium growths including names and images.Thx!!
@taylormadefungi9004
2 жыл бұрын
I fill the void on bottom side for those grey injection ports with RTV so they last a little bit longer
@AaronAlso
2 жыл бұрын
They are too cheap to worry about.... don't be an LC tipping barbarian and install a simple female luer lock to 1/8 barb fitting with a bit of silicone tubing (RTV and basic DIY required) Use a male luer lock plug to seal the lid. You can install the injection port, but will rarely use it. Just remove the plug, lock on syringe
@TheGoodFunGuy
2 жыл бұрын
Very informative vid, Gary! Thanks!
@pauloantunes8826
2 жыл бұрын
Gary!!!! I just made my first LC! Thank you a lot for your techings!
@Sssanbo
2 жыл бұрын
LC requires being super sterile. I remember being worried that i was contaminating the LC every time i even put the needle in, even when testing.
@ryanennis3199
2 жыл бұрын
Same haha
@shortchanged.
2 жыл бұрын
Yep
@ClownWhisper
Жыл бұрын
I spray everything with peroxide then alcohol wp mins later. I have been infection free for more than 5 years
@taylormadefungi9004
2 жыл бұрын
Right on man looking forward to getting some of your new strains
@ShawnTheRazor
Жыл бұрын
I purchased your Pink Oyster Rosebud LC and have been very happy with it. I haven't fruited it yet, but just enjoying watching it colonize grains, agar, and substrate currently. This video makes me wanna pour some black agar.
@FreshfromtheFarmFungi
Жыл бұрын
awesome! love to hear it 🍄❤️
@unclebobsbees4899
Жыл бұрын
Thank you, this was informative. Especially for the new kids, like me.😊
@Thomas.V.-223
2 жыл бұрын
Thanks man for the tips and tricks...
@user-do7lp9fl8i
2 жыл бұрын
Keep putting those videos out bro🥳🍄🍄🍄🍄
@johnriperti3127
2 жыл бұрын
Fascinating Gary, thanks!
@ClownWhisper
Жыл бұрын
And I got cultures that are totally foggy and are clean as a whistle although I do use nutritional yeast which can fog things up to
@jamesrapkins4935
2 жыл бұрын
Black Charcoal Agar! :P i must have this! thanks Gary! MUSHLOVE , will be eating your Turtle shell Beech in a week. Colorado oyster was deeelicious ty x
@wajahathusain1175
5 ай бұрын
Please tell rapid qc detection for mycelium culture
@brianbordenkircher52
Жыл бұрын
Thank you for the info!
@raytry69
11 ай бұрын
Tip: put your mic away from the flowhood, that will reduce noise.
@user-xb1ht4py2v
28 күн бұрын
great video. I have a few questiosn though. Is the purpose of puling ot a syringe full of LC then squirting it back in too mix up the culture? AT time 7:44 when you tip the jar over to fill the syringe alot of liquid drips out onto the table; why, what, and from where?
@boomingtrendz
9 ай бұрын
Hey, what’s up Gary very good informative video on liquid culture. Thanks for sharing this I do have a question what size are those ball mason jars? Thanks again for all you do in our community.
@FreshfromtheFarmFungi
8 ай бұрын
these ones were 1/2pints from walmart
@user-mz3cl2bh4g
5 ай бұрын
I'm having problems with contaminated lc. Who has the best golden teacher lc
@koltoncrane3099
Жыл бұрын
If you’re going to test your LC on agar, and grow them to ensure they’re clean and strong why then do you use little bottles? Wouldn’t it cut down or reduce your labor if you say used gallon jars and then did quality control tests on that gallon jar? That way you ensure that one jar is clean and good but then because it’s say a gallon you can get way more out of it. The expense per jar would be lower. In accounting they talk about fixed and variable costs. In this you could reduce your labor costs a lot per LC syringe you sell by going to large jars to reduce quality control costs
@FreshfromtheFarmFungi
Жыл бұрын
yes - mostly because of space issues and because I like to have fresh LC but at our new spot we will be scaling all of this thanks for the suggestion your head is in the right spot! 🙏🏻🍄👍
@CMAenergy
8 ай бұрын
I made a simple flow chamber But what is the best sized filter to use How many microns I think this one has 2 m Also on a Petri dish When there is contamination and you see some good spores . Is it possible to remove some good spores and see if they will produce without contamination I assume not but just a thought for a beginner
@buself
Жыл бұрын
hey, everyone says that you should store your agar plates upside down, but seems you don't do it. is it really needed?
@FreshfromtheFarmFungi
Жыл бұрын
long term yes to prevent condensation from forming a pool on the surface and choking the mycelium. For active growth it is best practice but isn’t going to make or break it. I do store them agar side up refrigerated for this reason.
@jerichojames4769
Жыл бұрын
does every withdrawal of liquid culture have the possibility of contaminating the entire liquid culture?
@FreshfromtheFarmFungi
Жыл бұрын
it has the possibility yes but if you use good protocol it is very small risk. Make sure you wipe with isopropyl and use aseptic techniques
@buself
Жыл бұрын
I have been constantly getting trichoderma on all my LC attempts.. My pressure cooker is only at 13 PSI, do you think that could be the issue? I sterilize my agar and LC for about 20 mins. Also I tried making agar plates(which is sterilize the same as my LCs) and left them without inoculating for few days - no green mold is growing. I am really confused and frustrated so far.
@thejohn6614
Жыл бұрын
Are you flame sterilizing your syringes when you're going into the lc? What do you have for a filter on the lc jar?
@blahzes
9 ай бұрын
Wouldn't it be cheaper to incoulate small grain jars?
@FreshfromtheFarmFungi
8 ай бұрын
yes but contaminants can hide in the 3D nature of a grain jar when plates filter out this dimension
@Basket_Knot
2 жыл бұрын
You look so much like a guitarist named Adam Demicco!
@FreshfromtheFarmFungi
2 жыл бұрын
neat! I used to play the trombone and bass guitar in high school so I appreciate the art
@BadgerLaser
Жыл бұрын
interesting video many questions answered and lots to think about. what is the idea with pumping the syringe several times please ?
@FreshfromtheFarmFungi
Жыл бұрын
it just primes the pump and mixes the culture so it’s more homogeneous
@kevdogg3606
2 жыл бұрын
If you have a syringe filter on your lid...do you leave the lid tight or back it off before placing it in the pressure cooker ?? Thanks for your time..
@FreshfromtheFarmFungi
2 жыл бұрын
I back it off a quarter turn just because it may loosen the seal
@judyausmus6130
Жыл бұрын
Like your video
@ystes23
Жыл бұрын
Do you put parafilm in the petri dishes after inoculating?
@sansulabrahma349
2 жыл бұрын
How many temperatures store
@trevorbraden5448
Жыл бұрын
What brand lids are those ?
@AndrewPak-kt6qy
Жыл бұрын
Once you've confirmed the viability of a culture, can you then use the same syringe you used to test? I want to reduce the number of times I have to poke into the jar and use less syringes. Thanks for all the info!
@FreshfromtheFarmFungi
Жыл бұрын
yes you can do that just do few ml’s each and there should be enough for both
@AndrewPak-kt6qy
Жыл бұрын
@@FreshfromtheFarmFungi Awesome, appreciate the reply and all the great vids!
@littlerhino2006
2 жыл бұрын
Okay what are the likely consequences of a home grower skipping this step, is the mycelium able to consume the bacteria or will the resulting mushrooms all be contaminated?
@FreshfromtheFarmFungi
2 жыл бұрын
it will probably cause contamination problems and be discouraging
@kicknadeadcat
2 жыл бұрын
I used honey and peptone 15 psi 30 mins and the LC was a little cloudy right out of the cooker. I have used honey alone and maple syrup alone and it was clear. Could it just be peptone particles? Mycelium seems to be growing.
@FreshfromtheFarmFungi
2 жыл бұрын
possibly - need to test on agar
@CharvelJackson
Жыл бұрын
Tks Gary, I think there are some of us with no flow hoods, so, this testing step introduces another vector that is independent of the original LC and seems like a way to get some false positives for contains which may not be there? I guess grain in a jar would be the best alternative for us non flowhooders?
@FreshfromtheFarmFungi
Жыл бұрын
no, you should still test, just sterilize the needle and injection ports right before testing (flame the needle and use iso on the port)
@CharvelJackson
Жыл бұрын
@@FreshfromtheFarmFungi Thanks for your time, I am not understanding how flaming and spraying needle and port when getting my LC out helps what seems like and Open aGar Dish when I open the lid to put LC in? I wont know if it was in the LC or if it came from the environment because I dont have sterile environment/lab. In grains I have the environment sterilized and the LC doesnt ever get exposed. ??
@spencerreiser7765
Жыл бұрын
Buy sterilized agar plates or make your own flow hood (SEE Also gordotek)
@eXWoLL
2 жыл бұрын
Is activated charcoal agar really good for QC? Because from what I've read about it, it's supposed to reduce/elminate bacterial growth, in which case would kinda of neglect its usefulness in QC since if its effective in its purpose, you wouldn't see any bacterial growth from a contaminated LC.
@FreshfromtheFarmFungi
2 жыл бұрын
From my experience black charcoal isn’t antimicrobial, it may slow the growth which would make it easier to discern different colonies before they all grow and merge into a larger biofilm but mostly it stabilizes the pH in the media and can help absorb some of the exudates from the mycelium to prolong the life
@Foxxxxx96
Жыл бұрын
@@FreshfromtheFarmFungi very interesting
@MayhemKeys
2 жыл бұрын
When isolating or germinating spores, do you ever use antibiotics?
@FreshfromtheFarmFungi
2 жыл бұрын
I don’t but it’s a good last resort
@zeuscaboose7202
2 жыл бұрын
How do you decon your plates for another use?
@FreshfromtheFarmFungi
2 жыл бұрын
We only use them with agar once, but then for spore printing we wipe them clean and spray down with isopropyl alcohol
@chadgurney1672
2 жыл бұрын
Where's a place to get liquid culture testing supplies
@FreshfromtheFarmFungi
2 жыл бұрын
amzn.to/331iD1c here these work well
@chadgurney1672
2 жыл бұрын
@@FreshfromtheFarmFungi thank's love the videos
@nickfranco2259
2 жыл бұрын
Why did the lc drip out while you where filling your syringe??
@FreshfromtheFarmFungi
2 жыл бұрын
the lid wasn’t on tight enough
@Nope-hz1ux
2 жыл бұрын
@@FreshfromtheFarmFungi it came from the filter when you were pumping the syringe back in. why are you pumping it like that anyway?
@antoniosa932
Жыл бұрын
@@Nope-hz1ux i saw that too, never seen that before
@jschultz353
Жыл бұрын
have I been mispronouncing agar this whole time?
@FreshfromtheFarmFungi
Жыл бұрын
people say that I have - midwesterners say “Aygar”. Upstate Ny’ers day “Ahgar” it’s all the same 🤷♂️
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