This is a great starter! In 1979 I worked as an intern in an analytical chemistry lab doing HPLC and I loved the technique, never forgot it. A few years ago I built a hobby lab, and bought a lot of Shimadzu LC-10 machines. Quite vintage 😊 Electronics all refurbished and OK, purchased LabSolutions PDA and now in the process of getting the pumps up and running. A bit costly with all the spare parts that are needed, but so nice getting it working! I will check out all your YT's! Thank you for your dedication and sharing of knowledge, I really appreciate it! 🔆🔆🔆🔆🔆🤗👌🏻🙏🏻
@WildWesternLizardWizard
3 жыл бұрын
Amazingly helpful! Especially for an RA working with HPLC who never took any HPLC classes.
@jacquelineestrada489
3 жыл бұрын
Same! I majored in Biochemistry but only learned it briefly from the textbook.
@explorerofgooplanets2516
2 жыл бұрын
I've been working only with a medium pressure chromatography system, because it is the one which is available in my lab. Despite this fact the exam questions require knowledge of running procedure for HPLC😑
@arrtwo1375
2 жыл бұрын
Just started working in a research lab and this is SUCH a helpful refresher. BIG thank you!
@panchalchirag22
3 жыл бұрын
I'm technician of analytical instruments and working with dissolution apparatus and other things, my interviewer is not expecting me to have much knowledge of HPLC but I think you have helped a lot 💁💁💁 Have a great day!!
@ximenaloperaavendano7072
3 ай бұрын
Love this!!!
@panchalchirag22
3 ай бұрын
So the update is, they wanted me to work on weekends with just 25% raise on existing. I asked my current company retained me for whatever they think was great and retained at 60% raise. In a way, HPLC video helped me a lot at the end. 😄😄😄😄
@prabhatgurjar5932
11 ай бұрын
Please keep creating such content. Bottom of my heart.. Thank you
@H3vdWa1t
9 ай бұрын
YES! Thank you! You explain things so well. I'm going to watch this entire lecture series and share it with my supervisor and professor. This was so necessary and so easy to understand. Love it.
@professor_k_mdluli_
3 ай бұрын
Loved it, was studying for my pharmacy related course and decided to check for a video which would enlighten me on HPLC. Certainly, this video proved to be more than effective to that end.
@lv5111
Жыл бұрын
Using it to prepare for a new job. Thanks alot.
@swardabandiwadekar7275
6 ай бұрын
This is so easy to understand and at the same time detailed explanation for a complex topic of HPLC. Highly recommend it for anyone looking for an introduction to HPLC. Thank you so much for putting it out❤
@jollyronacher
7 ай бұрын
I used HPLC in a pharma lab daily and I was curious to see based on my knowledge actually using it how it compares to your lecture, and I’ll say I learned a few extra things!
@Segaton
10 ай бұрын
This is great. Got a job doing downstream work and this helps clarify a lot of things.
@Jay-lq8ly
Жыл бұрын
Thank you for these lectures! Very helpful and thorough 🙂
@remingtonzarro7156
3 жыл бұрын
Thank You very much for sharing your lecture series! They are so simple and informative, and are fun to watch. Great resource and great reference I often use for work!
@prakashpaudel7652
2 жыл бұрын
Big thanks. I fully understood the basic concepts. The way of delivering the content is crystal clear and understandable.
@michaelkidega3409
3 жыл бұрын
This lecture was so enriching. Thank you so much.
@victornwankwo1333
2 жыл бұрын
You are a great teacher, really enjoyed the class
@ChemComplete
2 жыл бұрын
Glad you enjoyed it!
@thatsam-9880
2 жыл бұрын
Fantastic. Didn’t quite understand it well until now.
@akyadott
3 жыл бұрын
Very good introduction to HPLC!
@emmanuelchinonso2444
4 жыл бұрын
Explanation made hplc simple
@ashudeline
Жыл бұрын
Awesome video
@jordanisthebestable
3 жыл бұрын
Fantastic! Your efforts are much appreciated.
@giantbird
2 ай бұрын
Just ordered your guide.
@NiHnet
3 жыл бұрын
Thank you! Your lectures are helping me study for my exams
@nikkieasley6204
Жыл бұрын
This is so helpful!! Super clear and informative, thank you
@fratcangunalan1583
3 жыл бұрын
Thank you. It really help me to understand.
Жыл бұрын
This is so helpful. Much appreciated.
@behmaneshtara577
3 ай бұрын
It was really useful thanks a lot 🙏 😊
@anaoriondo5976
3 жыл бұрын
excellent video, VERY WELL EXPLAINED. Thank you so much
@ChemComplete
3 жыл бұрын
Glad it was helpful! Thanks for the feedback.
@tranll1
4 жыл бұрын
Thank you for the lecture!! Very clear explanation!!
@Anmol-bf3ml
3 жыл бұрын
Subscribed to your channel to not miss out on any awesome videos! Keep up the good work :)
@erikakormendi9896
4 жыл бұрын
Thank you, thank you, thank you🙏🏼
@ChemComplete
4 жыл бұрын
You are so welcome!
@olasharaki6255
2 жыл бұрын
Amazing and a simplified informative lecture...Thanks
@Daks-3
Жыл бұрын
Thanks so much. Very Educative
@messaoudiimadeddine9393
Жыл бұрын
Thanks for your time is very helpful
@kartikeyapandey1951
2 жыл бұрын
Amazing...just amazing
@aliciamariacarrillohereder5741
2 жыл бұрын
Extremely helpful, thank you! I have a question: is retention time calculated at the moment of the peak or where the curve of that substance begins (and therefore where it starts to come out of the column)?
@AKMirzaganj_2023
2 жыл бұрын
Hiiii where are you from
@Swashbucklebuckle
2 жыл бұрын
@@AKMirzaganj_2023 cringe
@nessanessa01
2 жыл бұрын
Peak
@spelicija
6 ай бұрын
Hvala.
@ramzankaleem4470
2 жыл бұрын
Great learning through this lecture
@yuki_kerk99
2 жыл бұрын
easy to understand, nice explanation!
@visalasreelakshmidevalla-ne2lv
7 ай бұрын
Good, it is really interesting...
@jellyannsampang9894
11 ай бұрын
subscribed! very informative video!
@lilmisschemist840
4 жыл бұрын
very good lecture!
@soheylasiavoshi7759
Жыл бұрын
Great course. Thank you so much ❤
@Mijnmening869
8 ай бұрын
You need a high temprature to push the compounds in. So does that mean the GC works with (air) pressure too? 2:34
@m_humayoonamini
3 жыл бұрын
very clear explanation, thank you very much
@amnahamad5080
Жыл бұрын
thank you so much for the lectures great job it assist a lot 🥰
@jocelynemmukendi1307
4 жыл бұрын
Great lecture
@rushiilr7079
2 ай бұрын
Thank you!!
@mehransoltani9356
3 жыл бұрын
Great content!
@ChemComplete
3 жыл бұрын
Thank you! These take a while to put together so I am happy to hear they are appreciated!
@Zayskibop
2 жыл бұрын
thank you for all of this!
@abhivivekthakur
6 ай бұрын
Great video!
@charmming1994
2 жыл бұрын
thank you very much . you are amazing
@ChemComplete
2 жыл бұрын
Happy to help
@hugotolentino4348
3 жыл бұрын
Amazing Lecture! Thank you
@enenenergp
3 жыл бұрын
This video was great! The more detailed picture of the HPLC system was great and clears things up a lot. I have a question about the chromatogram: Does one substance, I mean like one chemical compound, always only have one single peak in the chromatogram, and the other peaks must be something else?
@ChemComplete
3 жыл бұрын
In general, yes each unique compound will show up as a single peak on a chromatogram. That being said many times suboptimal conditions can lead to "peak tailing" which spreads out or gives the illusions of multiple peaks. The only time a compound might give 2 signals is if somehow it was breaking down into two separate sub-compounds when exposed to the HPLC conditions.
@enenenergp
3 жыл бұрын
@@ChemComplete Thanks so much for the reply! :)
@r1909
3 жыл бұрын
This was very helpful, thank you!
@maxz9787
2 жыл бұрын
Im currently doing an Internship at a company and my boss suggested to me I should write my bachelor thesis about hlpc analysis for a special kind of oligosaccharifes. Im a bit scared about the fact that nobody at the company including me worked with hlpc before. Can anyone give me advice If it is a good Idea??
@jnaniashok
Жыл бұрын
superb
@thgeamer8661
3 жыл бұрын
This was extremely helpful prepping/reminding me how the instrument fully works for my interview. thank you very much
@ChemComplete
3 жыл бұрын
Best of luck!
@Swashbucklebuckle
2 жыл бұрын
Same reason that brought me here! I need that in-depth info because at my university I never got to use the hplc myself...
@venkatrajnarayanan2785
4 жыл бұрын
Top Notch!!!
@spiderwomen8410
3 жыл бұрын
Thank you 😌🙏
@almamun825
4 жыл бұрын
Informative lecture. Thank you.
@HiNinqi
4 жыл бұрын
I really like your accent. Thanks for this explication!
@lisasevilla8061
3 жыл бұрын
Great video!! Thank you!!
@artemisaflores7578
2 жыл бұрын
Thank you for your video!!!!!
@smileanwar4746
3 жыл бұрын
Thanks
@ostadhasan69
11 ай бұрын
Was good❤
@yamawwad
4 жыл бұрын
Thanks a lot for the explanation. How many types of calibration curves are there that can be used to calculate the concentration of a certain constituent in a sample ?
@ChemComplete
4 жыл бұрын
There is technically an infinite number because you can use any variety of known concentrations. There are two major points to keep in mind when designing an unknown calibration curve: 1. You want to use multiple known concentrations with a good spread of values. I always teach a minimum of 5 known concentration values for a solid correlation. 2. The range of known concentrations must capture the unknown value. This is to say that the unknown concentration must fall between your lowest and highest known concentration.
@rockymountainape
9 ай бұрын
Do i need to have certification to operat this machine
@ergrthdfhdh3935
2 жыл бұрын
great..thank you
@hosseinchangaei9905
4 ай бұрын
Great
@explorerjaat9532
2 жыл бұрын
thanks man!
@Rodrigo-im2nk
Жыл бұрын
Great Class, please what software did you use to draw during the lecture ?
@qashangabdulsamad8235
3 жыл бұрын
thank you it was useful
@luketownsend391
2 жыл бұрын
great vid
@ladushky1
3 жыл бұрын
thanks duuude
@TherealLaserdog
Жыл бұрын
So cool
@iqrabashir5118
Жыл бұрын
Sir i have a question.. Does a compound that is to detect have same retention time while using different solvent system?
@cisemnildemkeskin4856
3 жыл бұрын
I would like to know why and how we use standarts of these samples?
@sarkarrudra
3 жыл бұрын
This is a great video will be waiting for more. I am using a reagent to convert dimethyl sulfide to dimethylsulfoxide, can use HPLC (as you have mentioned using calibration curve) to get catalytic turnover numbers?
@joshuadillard2263
2 жыл бұрын
you would probably be better served trying a GC method for this.
@ansarialtamash7669
6 ай бұрын
Im an Engineer who is working as a service engineer in hplc agilent modules. Being from the mechanical background I am weak in chemistry like what is polar, non polar, ionic bonds, chains, silica, different phase, etc so can amyone fuide me and explain it in easy words
@ashvirtewharie4454
3 жыл бұрын
Thank you
@ashvirtewharie4454
3 жыл бұрын
Keep this up.
@uf98uf
3 жыл бұрын
May God bless u
@reeljour884
4 жыл бұрын
thank you 😍
@APC-pm2on
4 жыл бұрын
thanks!!
@isabellacosta3933
4 жыл бұрын
Gostei!
@CowbaneDineshChandimal
22 күн бұрын
Gonzalez Deborah Lewis Jose Lee Matthew
@chitigori7349
2 жыл бұрын
sir i need the software of hplc.. of ( Hitachi ) company
@KddbBxjdnnd-j9k
11 күн бұрын
Thompson Patricia Anderson Brenda Miller Mark
@KimberlyMyers-s3f
19 күн бұрын
Lee Larry Clark Thomas Martin Kenneth
@billlivingston6976
6 күн бұрын
Davis Deborah Rodriguez David Harris Jessica
@HermanWilliams-j8t
11 күн бұрын
Lopez Margaret Walker Jose Young Thomas
@bxmx4627
Жыл бұрын
В институт привезли и установили этот аппарат, очень дорогой и грузом лежит безхозный. Дело в том что нету специалистов умеющих работать на этом приборе, с английским плоховатои нет руководства по эксплуотации. Если есть что то то на английском языке. Можно русифицировать прогграммное обеспечение этого прибора??????????
Пікірлер: 144