🔬 Science in Action: Tissue Dissociation Protocol! 🔬 Here’s a glimpse of the steps I followed:
1️⃣ Received 6 endometrial tissue samples in 2 ml eppendorf tubes.
2️⃣ Placed each tissue piece into separate wells of a 6-well plate.
3️⃣ Added 1 ml of DMEM media to each well.
4️⃣ Carefully sliced the tissue into small fragments using scissors.
5️⃣ Transferred the small tissue pieces into T-25 flasks.
6️⃣ Added 4 ml of DMEM media to the flasks.
7️⃣ Placed the flasks in a CO2 incubator at 37°C.
Over the span of 3-4 days, these cells will flourish, multiplying and thriving, as they adapt to their new surroundings and propagate within the nurturing DMEM media.
Only stem cells have the ability to divide and multiply which means that after a while all the other cells while die and only stem cells will survive!
Stay tuned for more exciting scientific adventures! 🌱✨ #ScienceProtocols #TissueDissociation #CellCultureMagic #LabLife #phd #cellculturelab #immunology #stemcell #stem #scicomm
Негізгі бет Isolation of Stem Cells. Science in Action: Tissue Dissociation Protocol!
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