Working on the weekend again, Linda??? You’ve really got to find a work/life balance
@iluhyuhswag
2 жыл бұрын
So helpful
@julyd2269
3 жыл бұрын
Wieso trägt man Handschuhe aber keine Schutzbrille ? Klarer Anfängerfehler
@mdhossain5564
3 жыл бұрын
Excellent! i WANT MORE VIDEOS!
@mcdonald6284
2 жыл бұрын
You didn't say the amount of Ethidium bromide added
@imago6116
Жыл бұрын
Shouldn't we put the gel into the apparatus and cover it with buffer before removing the comb in order to prevent it from damage?
@antiawarenessawarenessclub
6 жыл бұрын
Masking tape is such a wondrous invention
@satyris410
3 жыл бұрын
When pouring hot gels, I started using autoclave tape instead because it held up better under the heat!
@IScreenshotNFTs
3 жыл бұрын
Our lab usually buys ready-to-use gels. We don't deal with smaller DNA fragments or too large. So we order 2% agarose gels in bulk and get discounts. Maintains consistency, lowers contamination and its easy to replicate
@12823matthewkao
3 жыл бұрын
I got scolded by the postdoc when I shook the flask as she does in the video.
@meagansineadenright2661
2 жыл бұрын
what is the buffer solution? Can it be water?
@MickBReedy
15 жыл бұрын
simply amazing
@deegallarupananda6927
Жыл бұрын
Very simple and useful video
@valentinapeters8505
4 жыл бұрын
Why is she not wearing any eye protection?
@zawarudo93
Жыл бұрын
purrrr I'm here for my lab
@lHarchester
3 жыл бұрын
Very imformative. Thank you.
@ربيبالفؤاد
3 жыл бұрын
Why is appear dimer ?
@hanifazam1824
2 жыл бұрын
OUTSTANDING
@naluh_n
3 жыл бұрын
great film
@meganboldt7092
3 жыл бұрын
THANK YOU!
@chmou80
14 жыл бұрын
@jrwkc good.
@jrwkc
14 жыл бұрын
some labs really not afford gel casting rig? Masking tape? I would quit right there.
@satyris410
3 жыл бұрын
haha, I used to run gels 52 lanes wide, the tray was about the size of an A4 piece of paper and there were no casting holders big enough. I found, after more than one piece of masking tape failed, making a mess on the bench, I switched to using autoclave tape instead.
@arafelyb
8 жыл бұрын
nice explanation!
@Moonsabie
13 жыл бұрын
lovely
@jaywaijy
12 жыл бұрын
bravo!
@jupitersaturn1687
8 жыл бұрын
is that what they call liquid crystal?
@fabzy4L
3 жыл бұрын
no ma'am. liquid crystal is a structural anomally on the atomic level, not exactly related to molecular biology. liquid crystals areas follows: Google Crystal Lattice. This will show you how atoms arrange themselves in solid state. meaning, solids can arrange in a periodic-like pattern, or a totally amorphous structure (meaning with no particular shape, such as liquids). Plastics are most often disorganized atomically. Metals are most often highly crystalline. Liquid crystals imply there is still an ordered structure even in liquid state, which is not often the case. why? because heating solids to turn them into liquids, makes that crystal structure start breaking down, causing them to lose its arrangement. Of course, when it doesn't, it becomes a liquid crystal.
@maxdlv
12 жыл бұрын
Oeter
@YoghurtMcGee
13 жыл бұрын
@jrwkc hahaha I used masking tape in college to make the sides of the gel rig.
@fabzy4L
3 жыл бұрын
mmm, i REALLY don't think shooting microwaves at a protein for 1:30 minutes does a great job at maintaining structural integrity in the saccharides. how do i know? I conducted SE Microscopy on lyophilized samples.
@IScreenshotNFTs
3 жыл бұрын
Agarose isn't a protein. It's a sugar. Heating agaorse enables cross-linking. Think of an agarose gel like sieve where DNA fragments of different length can get separated.
@fabzy4L
3 жыл бұрын
@@IScreenshotNFTs i know. In my lab we used pink agarose with furfuryl gelatin, hence why i mentioned the protein. I was speaking from anecdotal experience. And as far as crosslinking went, we controlled micropore size, and microwaving gave a wide distribution among pore sizes, surface area & homogeneity. That’s often the case with polymers unless crosslinked from the inside out with divalent ions. Just my two cents, my man.
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