10:10 was the moment the advantage of two photon microscopy clicked for me. 16:37 is another great point.
@OsamaOSadolla
4 жыл бұрын
mvp
@1239752
5 жыл бұрын
Much better than my university's newbie lecturer(surprisingly hired at US top 30 university) Wish he watch this and learn how to teach.
@abdullahiibrahimuba1137
10 жыл бұрын
Very complicated but well explained technique. Good job!
@alvaroperona6134
3 жыл бұрын
Thank you so much for this video!! I was 100% lost on how the multiphoton system worked.Very clear explanation.
@sr2109
5 жыл бұрын
Using the talk for preparing for exam. Really helpful! Thank you
@sashatolstoy531
9 жыл бұрын
Excellent and easy-to-follow!
@fernandamonteiro5181
7 жыл бұрын
EXCELLET explanation!! no doubts left behind, very well organized and fulfilled with information! great job!
@TheWupperdo
Жыл бұрын
Excellent explanations--didn't realize how much I wanted to use multi-photon microscopy until seeing this video!
@johnbates2709
7 жыл бұрын
Great presentation, very clear, thank you
@ClaraL27
3 жыл бұрын
SO clear and helpful- thank you!
@beflatminor
7 жыл бұрын
Very well explained and helpful. Thank you!
@WALLEHua
7 жыл бұрын
excellent lecture! very clear and easy-to-follow
@kadamnagesh5155
5 жыл бұрын
Very good information.. Easy to understand.. Thanks
@TheHerofruitlunch
10 жыл бұрын
Thanks for this, the basic principles are clear for me now!
@colinbarnes705
2 ай бұрын
Still a great vid 10 years later
@muthuveljothi4619
8 ай бұрын
Extraordinarily explained 👍🏾
@Kimya_el_tabkh
3 жыл бұрын
Great lecture. Thanks
@1point689
6 ай бұрын
Great lecture!
@gVaiwa
6 жыл бұрын
Easily comprehensible, thanks a lot :)
@samanthaamat4976
10 жыл бұрын
Very thorough, thank you!
@deebadubbie
7 жыл бұрын
Thanks for a great talk!
@danielarmbruster94
3 жыл бұрын
Awesome, thank you!
@gillenzfluff8380
6 жыл бұрын
Amazing stuff thank you.
@mariaguadalupedelrocioherr9722
9 жыл бұрын
Thanks :D ... Good explanation.
@milophilic5927
2 жыл бұрын
Super helpful and logical! thanks !
@charme2377
6 жыл бұрын
excellent video..i have a question please,i want to do a fluorescent exerience with confical and 1 EMCCD camera ..what is the difference between using 2 camera or 2 ...thank you
@frankclausen2506
6 жыл бұрын
wow - It's so nice to se another way for using second harmonic, that just PTK. dubling.
@crewberggren7669
2 жыл бұрын
10/10 In every category
@artsmith1347
3 жыл бұрын
So this is ionizing radiation at wavelengths far longer than those for x-rays?
@HayagreevSivakumar
2 жыл бұрын
thank you
@prananditabiswas4599
9 жыл бұрын
Thanks for the excellent explanation. I have a doubt : two photons are two different quanta, even if they arrive simultaneously, would they not excite two different dye molecules with the same energy and not the same molecule with twice the energy ?
@kunlin579
6 жыл бұрын
From my understanding, that's the trick of 2 photon, at the focal plane, the density of the photons is so high that 2 photons will hit the same molecule. Outside of the focal plane, the likelihood of 2 photons hitting the same molecule goes down, therefore photo-bleaching is minimized outside of focal plane
@poodook
4 жыл бұрын
There is no real upper level (excited-state) corresponding to a single photon’s energy and therefore there will be no absorption.
@artsmith1347
3 жыл бұрын
Agreed: 1) Excellent explanation. 2) I had(?) the same doubt. It doesn't square with my understanding of how excited states happen ... the reaction of a molecule to being hit with half the energy needed to excite it would be: "hold my beer." It appears my understanding is incomplete because they are making it work.
@aidated
5 жыл бұрын
Brilliant! Thank you!
@oludhe7
6 жыл бұрын
Very good presentation
@poodook
4 жыл бұрын
22:40-22:45 this is not truly the reason, in my opinion. A more appropriate explanation would be that the two-photon cross-section is extremely low and thus high intensities are a necessity for inducing two-photon absorption. Two photons will always be present, but the absorption will not be stimulated unless there is a high density of photons, owing to the low cross-section. In fact, this cross-section will become orders of magnitude weaker for even higher multi-photon events.
@Abyssale90
8 жыл бұрын
THANK YOU!!!
@Rhioah
10 жыл бұрын
Thank you!
@zainhassep7908
4 жыл бұрын
Excellent
@hellenishikawa7841
9 жыл бұрын
HI it will be possible to have the ppt presentation for didactic propose? It will be nice! Thanks Dr. Hellen Ishikawa-Ankerhold LMU Munchen
@iBiologyTechniques
9 жыл бұрын
Hellen Ishikawa Dear Dr. Ishikawa-Ankerhold, unfortunately we are not able to give out the powerpoint presentations. If there are particular slides you are interested in let us know and we'll see what we can do!
@cokecamilo
9 жыл бұрын
Are you from the future!?
@dr.vannostrand411
10 жыл бұрын
any idea where i can get that graph at 6:25? the link to it doesn't work
@iBiologyTechniques
10 жыл бұрын
Hi Five-Sigma! You are right - the link doesn't seem to work anymore. I contacted the speaker and he suggested these two similar (but not the same) graphs: www.nature.com/icb/journal/v88/n4/fig_tab/icb2009116f1.html research.stowers-institute.org/microscopy/external/Technology/NLO/index.htm Sorry that we cannot find the exact one used in the talk!
@dr.vannostrand411
10 жыл бұрын
Thanks very much! Those graphs are great!
@edoardodimprima7519
3 жыл бұрын
@@iBiologyTechniques the links seem broken now. Thanks a lot for the great lecture!
@ashutoshmishra1554
3 жыл бұрын
Who dislikes such videos
@disisnups
6 жыл бұрын
good explanation, he speaks like sheldon cooper :)
@skoolscribe3918
3 жыл бұрын
Next time just present the source to your data and include user interactivity
@skoolscribe3918
3 жыл бұрын
So the goal is to measure photon from extraction rather than defining photon by compression however a photon compression engine will suffice most excellently for any security system weather it be for a spacecraft or for a human body.
@souvikces
7 жыл бұрын
Wonderful presentation, except the occasional tongue-clucking "sucking" sound (for instance, see 25:06, 26:19, 26:38, 26:50). This sound can be annoying for many audience. Thank you and I hope you take this suggestion positively.
@rockgaming1440
10 ай бұрын
This is violating Einstein's statement for photoelectric efect
@Manish00333
6 жыл бұрын
why is he so annoyingly excited by this
@BrandonSLockey
3 жыл бұрын
lmaoooo
@artsmith1347
3 жыл бұрын
Why are you annoyed? It is a well-delivered and interesting presentation.
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