I have been doing MTT from last few years now and i can tell u that it is one of the best video explanation of the assay. Nice job👍
@uzairali6427
3 жыл бұрын
Aoa bro. Can I have your contact email?
@yourdailydoseofreality3219
3 жыл бұрын
A very helpful video. The viability of positive control wells doesn't average to 100% in this presentation. I think it's because you calculated the average of raw positive control data before subtracting the average of the blank from the value of each well.
@atreyyu
3 жыл бұрын
Yes and because of that the relative viability of the samples are not % of the positive control as it was intended.
@halihammer
Жыл бұрын
Why is the blank value not also subtracted from the mean value of the positive control? Doesn't it also have the same DMSO background noise?
@tetrakfecn6864
2 жыл бұрын
So in my opinion, if you substract the mean absorbance of the negative control from the absorbance (exp), you also have to substract it from the poisitive control or it will falsify your results, because if you don't do that, technically a 100% viability isn't even possible... or am I missing something?
@atreyyu
3 жыл бұрын
Overall good presentation, however the formula should be corrected: the absorbance value of the blank wells (negative control) must be subtracted from all samples, including from the positive control samples. That's why your 100 % viability is not 100%.
@evangelinehermon2330
Жыл бұрын
The final concentration of MTT that you’ve used is 5ug/ml. Bt i guess it’s actually 0.2mg/ml - 0.5mg/ml. In our lab we’re routinely performing the assay and we use 0.5mg/ml concentration.
@saymaafrin5927
10 ай бұрын
Very helpful. Thank you!
@shubhamgarg2897
3 жыл бұрын
This is the first of its kind video with such an excellent expanation to MTT on youtube. However, I have a question related to the negative control. Does negative control include only DMSO or it should be MTT + DMSO?
@ahmed-lab
3 жыл бұрын
Thank you so much for the positive comment. The negative control should have no cells or drug treatments. You can do it two ways - you can either just add DMSO to it at the end and subtract the reading out as background. Or if you want to be more rigorous, you can add plain media to it (no cells or treatments), then add the MTT reagent mix, and then add just DMSO. The results should be similar since there are no cells so there will be no reduction or crystal formation. If you want to see more detailed versions of these videos, you can also check out additional videos (many more coming soon) here: kzitem.info/rock/zMh8ngHIzPB-bJCveXrhjg
@sarahhepler8654
8 ай бұрын
Can MTT be used for virus quantification?
@ahmed-lab
8 ай бұрын
No. However, it can indirectly measure toxicity induced by virus infection.
@ecstatic_wizard
Жыл бұрын
A fantasic video and explanation except that sickening vocal fry
@KairelEdwards
3 жыл бұрын
It has been a long time since I've seen such a well explained video. Truly exceptional!!
@harunal-rashidmando5177
Жыл бұрын
That was a splendid discussion. However, i was wondering if you do really enjoy your work, because your voice says otherwise.
@SadiaKhan-l4w
Күн бұрын
so positive control= cell+ mtt agent+ dmso and negative control = mtt+ media+ dmso only?
@AzminaAlisa
3 жыл бұрын
I love love love how detailed and direct the entire video is! Thank you so much!
@idrislangston882
3 жыл бұрын
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@zacharyzain3173
3 жыл бұрын
@Idris Langston instablaster :)
@idrislangston882
3 жыл бұрын
@Zachary Zain Thanks for your reply. I got to the site through google and I'm in the hacking process atm. Looks like it's gonna take a while so I will reply here later when my account password hopefully is recovered.
@idrislangston882
3 жыл бұрын
@Zachary Zain It did the trick and I actually got access to my account again. I am so happy! Thanks so much, you really help me out!
@zacharyzain3173
3 жыл бұрын
@Idris Langston No problem xD
@fatemehahangari3664
3 жыл бұрын
What a great channel you have! Everything a scientist need to know is available here. Thanks a lot & keep going.
@henryujeh4691
3 жыл бұрын
Your explanation is exceptionally clear and very detailed!
@mahtab998
3 жыл бұрын
This was the best MTT tutorial I have watched . Could you please do a video for ELISA and RT PCR? Thank you
@ahmed-lab
3 жыл бұрын
There is one for qPCR here: kzitem.info/news/bejne/pquOu6R_aZRepGk - Glad they are helpful to you!
@PriyankaYadav-gu7tc
3 жыл бұрын
I'm going to perform MTT assay using cancer cell Line, I want to know how can I decide concentration for any extracts or phytoconstituent to gate good results.. please reply me waiting for your answer Mam 😊🙏
@fatimohidowuojuade8401
Жыл бұрын
This video just explains everything I have being looking for in MTT assay. Thank you so much
@RoyalRoseTrue
3 жыл бұрын
A very tactful way to understand the metabolic activity of certain cell cultures. Good work.
@reystudio4317
4 жыл бұрын
Great video! The video explained many detail things and combined examples to better understand the technique. It is hard to find such high-quality video in youtube. Hope to provide more common skills and techniques in research. Thank you!
@teeteringintheunknown5601
4 жыл бұрын
WE ARE EXCITED TO SEE THAT THIS VIDEO PROTOCOL WAS USEFUL TO YOU.
@KidsTimewithFola3038
Жыл бұрын
You have taken your time to give a detailed explanation. good job and kudos to you
@francobrae
3 жыл бұрын
Thank you very much for the explanation. With which program did you do the graphs and IC50 calculation?
@ahmed-lab
3 жыл бұрын
We typically make our graphs and calculations using a combination of excel and prism. You can see more details about that in this video - kzitem.info/news/bejne/taSJp5iqq6l4fG0
@francobrae
2 жыл бұрын
Gracias
@ladyn24
4 жыл бұрын
hi could you please provide reference or article showing the % viability equation?
@saritapal9662
3 жыл бұрын
The step 2 Add MTT is not clear.. Can anyone please explain 1. Make 5mg/ml stock in PBS 2. From this dilute how much amount ? After this process is not visible
@Diogenesthedog0
3 жыл бұрын
Why you stopped making Videos? Your Voice is so good and easy to follow🙏🙏 Start uploading if possible... I hope everything is fine
@rajghosh4485
2 жыл бұрын
Can water be used as solvent for such cell culturing and performing various viability assays just as DMSO? If not then y?
@naribeatty1644
Жыл бұрын
This was a very helpful video! Where would you recommend purchasing the MTT assay, and would it include this protocol?
@karanthaker2191
3 жыл бұрын
So helpful! Thank you!
@TalythaAnggreyni--T
Жыл бұрын
why we have to do the dose or concentration of the drug as log scale? would you give me more explanation about this please. Thank you so much. Such a good video
@phshorooqqaz6541
Жыл бұрын
Very confusing 😢
@einsteingonzalez4336
3 жыл бұрын
This is the one I’ve been looking for after many years. Has anyone heard of a stereotypical laboratory that involves this same form of laboratory equipment? That’s why I came here.
@bilalraheemabdulraheem8445
7 ай бұрын
Thanks alot
@megaland4542
4 жыл бұрын
really nice I loved your approach
@devlin2002
3 жыл бұрын
You sound just like my friend Amy's soothing voice, which states for: piece of cake.
@nuratiqah1419
3 жыл бұрын
thanks for the very informative video..can i use an expired dmso for this assay?
@enassabry4971
2 жыл бұрын
It is one of the best vedios for explanation Mtt assay i have seen really perfect 👏👏👏
@sumitmanna3495
3 жыл бұрын
Very informative and helpful video to know all about MTT assay. Thank u..
@omarenriquefuertemor
2 жыл бұрын
This was highly informative and useful for what I'm doing. Thanks a lot.
@maki-chanyagami6842
11 ай бұрын
big help, thank youu!!
@EthanRiver
2 жыл бұрын
Very good presentation, helps me a lot! Thanks!!!
@trabdbioquimica
10 ай бұрын
Such an amazing video!
@basyalpalpa5680
Жыл бұрын
❤❤❤ exceptional
@이현서-z5u
3 жыл бұрын
It was really helpful and I really wanna say thank you to you all.
@madhulahan6788
3 жыл бұрын
So helpful for me. Thank you so much. No one can elaborate so nicely like did.
@saraibrahim8557
2 жыл бұрын
Much love & appreciation
@AmandeepKaur-cy8ds
2 жыл бұрын
Really impressive and informative content 👏
@karanthaker2191
3 жыл бұрын
Does this specific protocol have to be in 6 replicates, or okay to seed in triplicate?
@ahmed-lab
3 жыл бұрын
We usually do 6-8 replicates, just because MTTs can sometimes have higher error and more replicates helps manage the error bars, but 3 will also be ok if you get very consistent results.
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