Wonderful explanations. It is unbelievable how young you are and how well you know what you teach, apart from having excellent teaching skills. The world would be so different if teachers like you were teaching in our classrooms. Thanks for dedicating your life to this, thank you for helping us see things in science clearer, thank you !
@AKLECTURES
9 жыл бұрын
+dayhdez Thank you for those kind words! greatly appreciate that :)
@saltyburger5910
7 жыл бұрын
dayhdez he made a mistake at 8:39 btw.
@nilukiperera306
6 жыл бұрын
dayhdez I agree. Thank you so much!
@TheDuvee6
5 жыл бұрын
Young??!?!?! He's well in his 50s
@intanaprilianihasan3424
4 жыл бұрын
@@AKLECTURES nol
@sp-gy7dj
8 жыл бұрын
I learnt more in these 14 minutes than 2 weeks of lessons, wow thank you
@AKLECTURES
8 жыл бұрын
+Simona Peneva you're welcome! :)
@egberuarelydia1312
3 жыл бұрын
your videos lectures simply second to none on the internet.Always well explained.Thanks for all your videos
@phantom1014
9 жыл бұрын
You're a very good teacher. I take my hat off to you sir, and believe me when I say that I am extremely critical.. You deserve acknowledgment.
@janefrancesayogu2806
2 жыл бұрын
I must say a big thank you. you've helped through med school, with your lectures that are so easy to understand. i can literally listen to you and enter into the hall with pride and the results are always very fruitful. thank you so much for your lectures. it has helped me a lot.
@thomaskoh3289
8 жыл бұрын
Your videos are always of such high quality and grade it amazes me how knowledgeable you are! What is your occupation and job? I'm dying to know. Are you a teacher? Or are you doing this as a hobby. Either way, loving your videos. KEEP IT UP!
@berfindogan6167
3 жыл бұрын
Even though I am only in the 4. min. of the video, you explain it in a much more understandable and fluent way than my professor. thank you so much
@Snow4LifeNEver
6 жыл бұрын
Really clear explanations. Constant repetitions makes it really easy to follow and to retain the information. Love this teaching method, keep it up! Was able to watch at 2.3x and the pronunciation was still crystal clear!
@jennaertman9036
8 жыл бұрын
Very grateful I found this video! Crazy how much I pay in tuition at my college and yet I learned much more from this video than from my professor.
@Hafsa_668
3 жыл бұрын
Thanks AK you are one of best lecturer
@user_25815youtube
7 жыл бұрын
you will be the reason i get my pharmD degree. you are amazing. thank you for all your videos!
@AKLECTURES
7 жыл бұрын
you're welcome Sarah! best of luck with your studies!
@mohamedahmed996
2 жыл бұрын
did you get it?
@zieqahaziqah154
5 жыл бұрын
I"m so glad I found this channel. thank you so much!!!!!
@Urm0mz
2 жыл бұрын
My professor just pointed at the slides with his mouse and talking. I had no idea what he was talking about! Just a bit of showmanship and passion and it's all making sense
@dyaneribbink5520
8 жыл бұрын
I learn a lot by watching your videos. Thank you very much !
@AKLECTURES
8 жыл бұрын
+Dyane Ribbink you're welcome Dyane
@Chivende
5 ай бұрын
Wow ❤❤❤...im ....wow..this is wow .... I'm preparing for end of years this is awesome😊😊😊
@bmrasta5943
9 жыл бұрын
Great source of information! Thanks for taking the time to spread education on recombinant DNA technologies.
@timsy6869
9 жыл бұрын
Wonderful.Claps Claps Claps. I love the way you explained. Very simply you clear this thing in my head. Thank you. Iam waiting for more vidoes like how to read the DNA digestion, why we do it etc etc.
@MrRoperete
7 жыл бұрын
How does this only have 702 likes? THUMBS UP!! This is amazingly useful. Thank you for dedicating your time, hope you get some money from it!
@iveeeffy9406
8 жыл бұрын
by far the best video on this material!
@zizihassan3437
7 жыл бұрын
my prof just explain the lecture yesterday, i didnt understood completely,now i can understand it even better than my prof 😂😂😂 , thank u thank u thank u u r perfect man god bless u
@samad934
8 жыл бұрын
thank you very much for these lectures. every day i watch these videos. i am a biochemistry student. and these videos help me a lot. again 1000 thanks.
@riamirto5002
2 жыл бұрын
You are the best.!!!You do amazing work! Thank you for sharing
@junczhang
8 жыл бұрын
ive been watching your videos for months. Thanks!!
@righettilea
8 жыл бұрын
Thank you so much! It helped a lot. With your videos I will be now able to understand my classes. Thank you again and keep doing this
@carolinemangare3071
7 жыл бұрын
Great explanations, much appreciated.
@abadinino
7 жыл бұрын
Wow, I found it very helpful and the way you presented it is outstanding. Keep the good work
@uwunayeon9939
4 жыл бұрын
Very helpful! Much needed for my upcoming exam
@classylilashley
8 жыл бұрын
Your videos are THE most informative & clearly explained biology/biochem videos I've ever come across! Thank you so much!
@righettilea
8 жыл бұрын
Thank you so much! It helped a lot. With your videos I will be now able to understand my classes. Thank you again and keep doing this 😊
@purnimasaxena7617
7 жыл бұрын
your all lectures are very good ,conceptual and informative which make us fell biology interesting. Sir. i had a doubt.... the plasmid pbr322 which we are using is extracted from E.coli which already has resistance to both ampicillin and tetracycline. After insertional inactivation, ampicillin resistance is no longer and when this is reinserted in the host cells(mostly again E.coli which has dual anti biotics resistance) so how will you differentiate them now?? if you use tetracycline in culture medium then none will die and if you use ampicillin our desired cells will die???
@bill3430
Жыл бұрын
Yes I thought that too
@eatandrepeatt
4 жыл бұрын
Now I gain answer of my question topic...... Thank you Sir.......
@samad934
8 жыл бұрын
thank you very much for these lectures. every day i watch these videos. i am a biochemistry student. and these videos help me a lot. again 1000 thanks. :)
@asanda6315
4 жыл бұрын
This was so helpful..your explanations are so clear, Thank you!
@RaMa-ct5qo
7 жыл бұрын
Thank you so much ❤️❤️🌷you are amazing I wish all the best for you 🙏🏻I have an exam and your videos was so helpful
@retruthdecliesny4435
3 жыл бұрын
Thanks for this, I learnt a lot, you earned a subscriber
@Curious_Human954
5 жыл бұрын
You are a great teacher!
@veronicacarvajal4138
7 жыл бұрын
What is the significance of inactivating the green gene and having the cell pick up that plasmid, when both the green gene and blue gene were activated to begin with? Wouldnt a cell want a plasmid that enables resistance to both ampicillin and tetracycline?
@H-Bfit
3 жыл бұрын
If the genes become inactive, thn how the recombinant dna is used for further purposes lets say production of insulin?????
@GK-ef1ve
8 жыл бұрын
wish I'd known about this channel earlier
@irenevitali6187
3 жыл бұрын
how can we get the little DNA fragment to locate into the plasmid if the restriction enzymes cut the DNA molecole without create a little fragment? I don't know if I express my doubt in a clear way...
@darkmatter1900
7 жыл бұрын
You're a very talented teacher, thank you! :D
@sararizk5502
5 жыл бұрын
You are a saviour!
@ChinchillaHappyLife
6 жыл бұрын
Really good lectures!!!
@km2052
8 жыл бұрын
awesome as always
@g.sudharsangovindaraj9634
7 жыл бұрын
must check it out!!!!!! explanations are mind blowing and interesting!!!!!!! go AK!!!!!!!!!!
@kareshma89
6 жыл бұрын
thanks alot but can you please state the difference in transformation between gram +ve and gram -ve bacteria
@rahafrahal5960
7 жыл бұрын
you are amazing, i like your videos so much
@yasiralruwaili4315
8 жыл бұрын
This is just perfect. Thank you a lot.
@georgel8411
7 жыл бұрын
top notch instructor!
@maryamtahir231
4 жыл бұрын
The recombinant plasmid is inserted back into the same bacteria from which the actual plasmid was taken or is inserted into some other bacterial cell?
@kirankavin2826
6 жыл бұрын
crystal clear!! thank you so much ak!!!!!!!!!!
@thedownwarddoug6642
3 жыл бұрын
Damn. This is good
@cattycatterson
9 жыл бұрын
How do you distinguish between the transformants and the non transformants?
@arungoutham3981
8 жыл бұрын
grt and best lecture..........
@haroonsarwarful
7 жыл бұрын
How do you know that the cells that didn't take up the plasmids with the DNA fragment already have the gene for tetracycline resistance proteins?
@nazmusshalehin2872
2 жыл бұрын
Pbr322 is a plasmid that is resistant to both ampicillin and tetracycline
@meamea6540
5 жыл бұрын
@mayaclasses6124
7 жыл бұрын
sir can u tell us the name of person who fist discoverd plasmids and about name of plasmid pbr322 it means somthing or not.this video is very useful for us thank u sir.
@manaljarrar
6 ай бұрын
Great👍
@bibhadahal7392
8 жыл бұрын
Best explanation ever..thank you..keep uploading :)
@CitraTheKrumZ
8 жыл бұрын
dear AK Lectures, I have a question about the plasmid. naturally before it's inserted to desired gene, does pBR322 actively produce both protein for ampicillin and tetracycline? thus when the plasmid take up the inserted gene on PstI site it will knock off the resistance for ampicillin. but how do we isolate the cell that uptake inserted gene from the whole cells when they died in ampicillin solution? thanks
@inchyokk
8 жыл бұрын
+CitraTheKrumZ great question
@arnes3418
8 жыл бұрын
+CitraTheKrumZ you dont treat the cells with ampicillin cause all the cells, including the ones who took up the inserted gene, would die. you treat all the cells with tetracycline and only the ones who have taken up the plasmid (and the inserted gene) will survive cause the ones who didnt take up the plasmid dont have the tetracycline resistance and will die.. hope this helps
@CitraTheKrumZ
8 жыл бұрын
+Arne Scheire thank you for the explanation, however isn't all the plasmid has tetracycline site? Thus both plasmid that took the Inserted gene and the one who doesn't will not die to tetracycline solution.
@arnes3418
8 жыл бұрын
+CitraTheKrumZ but dont all the plasmids take up the gene?
@CitraTheKrumZ
8 жыл бұрын
+Arne Scheire no, not all plasmid took up the gene fragment. That's why purification Of cell that contain plasmid+gene fragment is crucial. My question is how to collect cell/bacteria that contain plasmid inserted Pst1 when these cell dies in ampicillin solution, while both plasmid contain Pst1 and plasmid only survives tetracycline?
@TheRealDanna
9 жыл бұрын
Thank you for explaining this in a way I can understand. I have a question, where are you from, Andrey? USSR?
@rittenbrake1613
5 жыл бұрын
blue- took up , got it , thank you sir !
@sanahameed9832
5 жыл бұрын
lol, its blue - not took up, perhaps you might want to rewatch that part, cheers
@rittenbrake1613
5 жыл бұрын
I think u misunderstood what I mean
@AmNomNom11
4 жыл бұрын
thank you!!
@LachlanV666
8 жыл бұрын
Sorry but what I can't get my head around is, why do we even want to clone/amplify/make lots copies of the 'gene of interest'?? What is the purpose of making lots of copies of them?
@Palamaopa
8 жыл бұрын
Well an example of when you would need high numbers of a gene of interest is if the gene codes for some sort of hormone that can be used in treatment. Medical facilities would need large numbers of the hormone so you would need to mass produce the hormone.
@andywong893
8 жыл бұрын
HOLY FUCKING SHIT! YOURE MY LIFESAVER!
@Wirasnita
6 жыл бұрын
I wanna know, Is RE efficiently cut all cell? and it possibility that the RE cuts in cell DNA not in target DNA plasmid?
@adamwilson301
8 жыл бұрын
Very helpful! Thanks!
@AAG414
4 жыл бұрын
better than my biology teacher ahaahha
@Fsology
8 жыл бұрын
thank you
@asap_itty
8 жыл бұрын
I don't understand why this is necessary. Why would you use this instead of PCR if the end goal is to just amplify the DNA? Wouldn't PCR be faster and easier? or am I missing something?
@AKLECTURES
8 жыл бұрын
PCR wasn't always around ... Plus PCR has limitations.
@2incheslang
8 жыл бұрын
the end goal isn't just to amplify DNA. Once the plasmid is inserted into a host cell, the genes are expressed which causes the synthesis of proteins or enzymes that the gene of interest codes for. A practical application of this is in the synthesis of insulin.
@2incheslang
8 жыл бұрын
the end goal isn't just to amplify DNA. Once the plasmid is inserted into a host cell, the genes are expressed which causes the synthesis of proteins or enzymes that the gene of interest codes for. A practical application of this is in the synthesis of insulin.
@LachlanV666
8 жыл бұрын
What I can't get my head around is, why do we even want to clone/amplify/make lots copies of the 'gene of interest'?? What is the purpose of making lots of copies of them?
@g.sudharsangovindaraj9634
7 жыл бұрын
The motive is to introduce multiple strands of the "gene of interest" into the host bacterial cells, several ones at a time. the bacterial cells, henceforth , begin to synthesise the enzymes or protiens that the modified plasmid vectors were coded for , which is what we want. thus , amplification is just the starting part of all these.. hope it cleared your doubt.............
@nurshahirahroslan3720
8 жыл бұрын
So good!
@RandyB1296
5 жыл бұрын
Thank you so much! Please come teach my bio class!
@somiaabdelgawad1085
8 жыл бұрын
Thank you so much that was awsome
@francinarapitse1930
8 жыл бұрын
thank you!
@CKsZoology
8 жыл бұрын
sir, recombinant DNA has been added to ampicillin resistant site then how the tetracyclin resistance has been suppressed??
@YuukiHirata23
8 жыл бұрын
+Charu Kataria I can understand what you mean. In this context, all cells will survive in tetracyclin solution. Because tetracyclin resistance is intact. Otherwise, in ampicillin solution, cells with modified plasmid cannot survive. Because some of cells lose resistance to ampicillin. Am I wrong with this?
@nefsa84
8 жыл бұрын
Okay I see, so maybe he made a small mistake. I would assume they would all survive too. So (to repeat what you said), if the PstI restriction endonuclease adds DNA fragments, it will inactivate the ampicilin resistant gene. So in a solution of ampicilin the modified plasmid will die
@liitbro
6 жыл бұрын
I think it’s not about whether the plasmid has taken up the gene of interest, but instead whether the plasmid is taken up by the bacteria, as antibiotics are meant to treat cells and plasmid is a dna.
@jacquiemitchell8384
3 ай бұрын
Send to self 2
@이명후-v7v
7 жыл бұрын
clone we just done by today but we need to have more technologie of clone
@Procrastinerd
9 жыл бұрын
Is it possible for cells to uptake both recombinant plasmids, as to attain both an ampicillin and tetracycline resistance?
@jillianjones4084
9 жыл бұрын
Cognitive Philosophy There is typically some type of purification step before the new plasmid is introduced to the cells.
@Procrastinerd
9 жыл бұрын
Jillian Jones So yes or no?
@jillianjones4084
9 жыл бұрын
No.
@666Necrocide
9 жыл бұрын
Cognitive Philosophy Yes, it is possible, note the EcoR1 restriction spot on the PBR322, you could insert your gene there and retain both resistances, however, you would have no (simple) way of testing whether it was successful or not. So you might end up later on with a giant batch culture of stock E.coli instead of (whatever you wanted).
@666Necrocide
9 жыл бұрын
666Necrocide Actually I just re-read your question and see what you mean... I don't think that using both plasmids simultaneously would be possible because they would overlap on the E. coli genome. So one would be selected and one discarded.
@malikbasharat7119
8 жыл бұрын
Hahaha.sir .it realyy makes me laugh.cx wotx da fun of creating the books.VN ua lectures are present here....ua amazingggg.sir I wana become like u
@AKLECTURES
8 жыл бұрын
+Malik Basharat Don't underestimate books.
@shabdulishinde3685
7 жыл бұрын
I was an idiot in class to gloss over these topics when they were taught. Now I need to go to KZitem videos for help because opening books to learn this puts me straight to sleep.
@thebluecapsule
6 жыл бұрын
you are awsome!
@federicomaidana6823
4 жыл бұрын
You make me want to study bro
@lubnasaqer9300
9 жыл бұрын
Hey, Why do we use plasmids instead of PCR?
@khandakershadia6462
9 жыл бұрын
lubna saqer By PCR you can multiply the number of DNA but you will not get the protein. To get the protein by the process of translation you need a organism. Insertion of the genetic material in a organism is done by using plasmid as a vehicle.
@lubnasaqer9300
9 жыл бұрын
+Khandaker Shadia thank u very much
@au2611
3 жыл бұрын
Gene inactivated solution won’t turn blu
@mayamaya-ry3eg
8 жыл бұрын
mantap gaaaaan
@mariahslittlelamb8049
7 жыл бұрын
I love you.
@dayvud8642
4 жыл бұрын
my fucken guy
@GrammeStudio
7 жыл бұрын
How does he NEVER stutter!? wtf?
@FeyTheBin
6 жыл бұрын
Take a shot every time you hear "plasmid"
@fancannoiran
4 жыл бұрын
This dude is Hannibal Lecter
@alexanderhewitt4345
6 жыл бұрын
Am I the only one thinks this guy has to be a genius?
@feliciascott83
6 жыл бұрын
Alexander Hewitt he's reading from a board
@MamadouNdiaye-eyo
7 жыл бұрын
in french please!!!!!
@shamymanavie5096
6 жыл бұрын
Why do i have some gay fantasy with this guy? lol
@ally850
7 жыл бұрын
Stop talking with your hands
@praisypaj
7 жыл бұрын
just fuck off
@samad934
8 жыл бұрын
thank you very much for these lectures. every day i watch these videos. i am a biochemistry student. and these videos help me a lot. again 1000 thanks.
@righettilea
8 жыл бұрын
Thank you so much! It helped a lot. With your videos I will be now able to understand my classes. Thank you again and keep doing this 😊
@laurilintott4754
9 жыл бұрын
Maybe you cover this in a future lecture but you have glossed over the main difference between pBR322 and pUC18, and that is how you determine which cells have taken up a recombinant plasmid (as apposed to taking up just the starting plasmid). In pBR322 you must use replica plating to determine which cells have taken up recombinant plasmid vs which cells have taken up the starting plasmid. For example, if you were cloning into the tetracycline gene, you would first select with ampicillin in order to identify which cells took up the pBR322 plasmid. You would then check the ampicillin colonies to determine which ones were no longer tetracycline resistant. Ampicillin resistant and tetracycline sensitive cells will have the recombinant plasmid. If the cells are resistant to both antibiotics, then they took up non recombinant pBR322. With pUC18 you can tell in a single step which colonies took up recombinant plasmid because these cells will be ampicillin resistant but will not turn blue because the inserted sequence has disrupted the B-gal. pUC18 has several other advantages over pBR322 but the ability to screen for recombinant plasmid in one step instead of two is a major advantage.
@samad934
8 жыл бұрын
thank you very much for these lectures. every day i watch these videos. i am a biochemistry student. and these videos help me a lot.
@marissam.6376
6 жыл бұрын
YOU ARE AMAZING!!! Wish I knew about your channel 10 years ago!!
@nefsa84
8 жыл бұрын
I'm confused at 8:40. I thought the original plasmid had resistance to ampiciln AND tetracycline. Why does the modified plasmid only now have the ability to resist tetracycline. I thought it already did...
@Palamaopa
8 жыл бұрын
You're right. What he's saying is that now it can only resist tetracycline, not ampicillin, because the insert inactivated the ampicillin resistance gene.
@saltyburger5910
7 жыл бұрын
Nefsa yes you are correct, that folk did committed a mistake. The gene which took the desired dna fragment will actually die when amphicillin is introduced to their medium.
@lenay3054
7 жыл бұрын
isn't that what he said? I don't get where he made a mistake..
@CombatHackerX
6 жыл бұрын
This is for the future comrades watching. I too, was confused by this, but we just did not have the full picture about how the process works. Essentially, in most examples, they use one selectable marker(antibiotic resistance section) to simplify things and have the cut site be at the MCS. Anyway, in the video's case, once you have the end product (ampicilin resistance gene inactivated/tetracycline activated) you would introduce it into a bacterial culture. To simplify things, you can assume that initial bacterial culture does not have any plasmids inside at all. Thus, SOME bacterial cells will take up your plasmid with tetracycline resistance while MOST bacterial cells will have NOTHING INSIDE THEM(blanks)//will not have the antibiotic resistance. Therefore, once you introduce the tetracycline, you will kill off the blanks while only keeping the cells that have taken up your plasmid. At least, that is how my cell bio class simplified it. I think Luari's answer tho was more informative/detailed "Maybe you cover this in a future lecture but you have glossed over the main difference between pBR322 and pUC18, and that is how you determine which cells have taken up a recombinant plasmid (as apposed to taking up just the starting plasmid). In pBR322 you must use replica plating to determine which cells have taken up recombinant plasmid vs which cells have taken up the starting plasmid. For example, if you were cloning into the tetracycline gene, you would first select with ampicillin in order to identify which cells took up the pBR322 plasmid. You would then check the ampicillin colonies to determine which ones were no longer tetracycline resistant. Ampicillin resistant and tetracycline sensitive cells will have the recombinant plasmid. If the cells are resistant to both antibiotics, then they took up non recombinant pBR322. With pUC18 you can tell in a single step which colonies took up recombinant plasmid because these cells will be ampicillin resistant but will not turn blue because the inserted sequence has disrupted the B-gal. pUC18 has several other advantages over pBR322 but the ability to screen for recombinant plasmid in one step instead of two is a major advantage."
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