Your videos are very useful, I greatly appreciate them!
@damienharkin
2 жыл бұрын
You are most welcome. Thanks for watching!
@aamirrashidkhan3514
2 жыл бұрын
Thanks Damien and Chris, wonderful job!! very useful video for early learners!
@damienharkin
Жыл бұрын
Glad it was helpful!
@glendaoliveira5342
2 жыл бұрын
Very helpful. Thank you very much
@afraalsamkari2445
2 жыл бұрын
Amazing 🤩. Thank you so much.
@nabarcheto7950
Жыл бұрын
Thank you so much! So well and clearly explained! Really, thank you!
@damienharkin
Жыл бұрын
Thanks Na. Glad you found the video useful. If you are interested, additional content on frozen sections can be accessed from my online course by joining my channel as a “trainee” member.
@janvimore3057
2 жыл бұрын
Thank you Sir.... It's really helpful
@astherfe
3 ай бұрын
As an anatomic pathologist. Much appreciation for this video! Hope you can sample other tissue such as lymph node.
@damienharkin
3 ай бұрын
Many thanks for the positive feedback. Since we’re a teaching laboratory we don’t often get access to fresh lymph nodes but this is a great idea for future videos.
@aderemiaishah2129
2 жыл бұрын
So educative
@coastalplayer
3 жыл бұрын
ASCP 30 years fromTexas,very nice technique
@user-yd2ff4ms7l
2 жыл бұрын
very well, thank you very much
@joelscott7351
3 жыл бұрын
Nice vid always wondered what that anti roll plate was for as ones at work dont have the glass piece
@Viralvangaad
3 ай бұрын
So detailed, Thank you
@damienharkin
3 ай бұрын
You’re welcome 😊
@asalawati
Жыл бұрын
👍👍👍
@yijingwang7308
2 жыл бұрын
Hello, I wonder will you dry out your slides at Room temperature after cutting?
@captaincrunch5567
2 жыл бұрын
does tissue sectioning for ovary also uses cryostat?
@linhmaithuy6531
6 ай бұрын
Have a great day sir. I have a question: with spleen, lymph node specimens, should the section after being embedded in paraffin be cut thinner or thicker than usual ? And why we shoud do that ? Thanks for answering me !
@damienharkin
6 ай бұрын
I think this depends on a number of things. Lymphoid tissue is generally more fragile owing to less ECM components and higher density of cells, so you may gain advantage by cutting slightly thicker sections, but generally this issue can be addressed via use of picric acid in fixative solutions such as Bouin’s.
@linhmaithuy6531
6 ай бұрын
@@damienharkin yeah i got it. Thank u so much.
@jmslrnz
Жыл бұрын
What is the embedding medium for frozen sections called?
@damienharkin
Жыл бұрын
Hi James, most laboratories use a commercial embedding medium known as O.C.T. which is an abbreviation for Optimal Cutting Temperature. OCT contains polyethylene glycol and polyvinyl alcohol dissolved in water. You can buy OCT from a variety of scientific suppliers.
@noahriggs455
17 күн бұрын
Negative -50
@udithanilmini7613
Жыл бұрын
What is the thickness of sections ?
@damienharkin
Жыл бұрын
The thickness varies a little according to the tissue but generally we cut around 6-8 microns.
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