Great video, but are there any ways to assign the samples to wells faster than clicking the well and it's respective sample ID from the left panel? I ask because I run full plates of individual sample names and it's quite the tedious task.
@LearnInnovativelywithMe
Ай бұрын
Thank you! I'm glad you enjoyed the video. Assigning samples to wells can indeed be tedious. One way to speed up the process is by using a plate layout template or a batch assignment feature if your software supports it. Many plate readers and software programs allow you to import a list of sample IDs and their corresponding well positions from a CSV or Excel file, which can save a lot of time. Check if your software has this capability, as it can streamline the assignment process significantly. If you have any further questions, feel free to ask!
@prasoonmadhukar
2 жыл бұрын
Very informative 👌
@LearnInnovativelywithMe
2 жыл бұрын
Thanks Prasoon
@priyanksonagara2808
4 ай бұрын
Sir what is target and sample i dont understand it?
@LearnInnovativelywithMe
4 ай бұрын
In real-time PCR, "target" refers to the specific DNA sequence or gene that you're interested in amplifying and quantifying. This could be a gene associated with a disease, a genetic marker, or any specific DNA sequence you want to study. On the other hand, "sample" refers to the biological material or substance from which you're extracting DNA to analyze. This could be anything from blood, tissue, saliva, or even environmental samples like soil or water. So, in real-time PCR, you're essentially trying to detect and quantify the amount of a specific DNA target present in your sample. This technique is incredibly valuable in various fields such as medical diagnostics, environmental monitoring, and genetic research. Hope this helps clarify things for you! If you have any more questions, feel free to ask.
@tsogjargalburentogtokh7786
2 жыл бұрын
hello, thanks for the video. i have question. i don’t understand which quantitation method to choose? how to choose?
@LearnInnovativelywithMe
2 жыл бұрын
Hi You can get information about Experiment Type selection or Quantitation Method from ABI 7500 software help section. For this open the software and right click on help and open 7500 Software help This will open the new window of help and here in the Contents section you can get all information. But here in brief (Source ABI 7500 Software Help) 1. Standard Curve Experiments Perform a standard curve experiment to use standards to determine the absolute quantity of a target in samples. 2. Relative Standard Curve Experiments Perform a relative standard curve experiment to use standards and an endogenous control to determine the quantity of target in a sample relative to the quantity of target in a reference sample. 3. Comparative Cт Experiments Perform a comparative Cт (DDCт) experiment to use an endogenous control to determine the quantity of target in a sample relative to the quantity of target in a reference sample. 4. Melt Curve Experiments Perform a melt curve experiment to determine the melting temperature of a target nucleic acid sequence and identify nonspecific PCR amplification. 5 Genotyping Experiments Perform a genotyping experiment to detect single nucleotide polymorphism variants of a target nucleic acid sequence in samples. 6. Presence/Absence Experiments Perform a presence/absence experiment to detect a target nucleic acid sequence in a sample. I hope this may help you
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