I have never seen such an informative and comprehensive video on qRT-PCR. Thank you so much, ma'am.
@planthealth1396
11 ай бұрын
18:20
@user-zc6jd4fu5h
4 ай бұрын
Not to be dramatic but this video changed my life. Thank you so much.
@kokobtesfay-sk6ek
2 ай бұрын
I Am A LAB Techinital and I werked For 3 yers and am learnig adegree program so thank you for this pcr system used you continu I like you Dr Alexandra
@amirhosseinzarei2597
2 ай бұрын
Actually I have never found such an expertised content anywhere! I really do appreciate the quality of both information and quality of images!
@ginikillah1900
Ай бұрын
Awesome video on qRT-PCR. Learnt alot for personal development. Thank you Dr Lexa Scupham for this educational video as a lab personnel. Thumbs to you and your team.
@sergiosimoes1870
Жыл бұрын
Great in depth video about qPCR set up and data interpretation. Extremely useful for beginners and a great refresher for more experienced users. Lots of great practical tips and theoretical considerations. Dr. Scupham, you rock!
@umbrastudio8069
2 ай бұрын
I am a biology graduate and working as an laboratory analyst for 1.5 years. For I only working with traditional microbiology testing procedures, it is so hard for me to understand how PCR works. This video helps me a lot as a total beginner in molecular biology. Thank you USDA and Dr. Alexandra.
@lukebeckstrom2350
Жыл бұрын
this is by far the best PCR demonstration I've seen. Thank you!
@VinayHallurmicrobiology
2 жыл бұрын
One of the best videos I have seen to date!
@jaswinderkaursaini7407
2 жыл бұрын
Thank you very much for this detailed and comprehensive video, Ma'am. Please keep creating and sharing more such videos.
@fernandaaguiar9934
Жыл бұрын
Thank you so much, Dr. Lexa Scupham, for this amazing video. It is clear and very informative. I took some notes to help me with my experiments and protocols. I can say that now I understand how real-time PCR is performed.
@kathypakzad5367
11 ай бұрын
The most comprehensive video on RT-PCR I have ever seen. Thank you very much Dr. Lexa Scupham 🙏
@_TravelWithLove
3 ай бұрын
Thank you very much for sharing your insights and knowledge filled videos !! Professional and informative !! Outstanding !! Greetings from California … I wish you and folks good health , success and happiness !! Much Love ✌️😎💕
@communityhealth5003
4 ай бұрын
OMG .... what an excellent video
@praisehonour
Жыл бұрын
Great and in depth video about qPCR. It is really helpful and informative to beginners like me. Thank you Dr. Scupham.
@gbheo3571
3 жыл бұрын
Great demonstration to teach newcomers to PCR protocol
@user-ob6ls4vf8s
2 ай бұрын
Very detail and informative , thanks a lot.
@marziehdavoudi4004
Жыл бұрын
THE BEST TUTORIAL I HAVE EVER WATCHED. YOU ARE GREAT
@sahreensumaira6071
Жыл бұрын
you did a great effort Dr. I must say that it is one of the best tutorial with practical information and details on real-time PCR. thankyou so much. you rocked. Dr. Sumaira
@dereksniper
4 ай бұрын
very good and thorough video. Better than some undergrad labs. Thank you
@aymanmousa5600
3 ай бұрын
Wonderful thanks for your great work
@naeemaziz7613
4 ай бұрын
you really nailed it amazing each and everything very well explained it helped me in my research a lot
@mariaagustin5368
Жыл бұрын
Thank you Dr. Scupham for this wonderful video.
@user-xm4up5jp9m
3 ай бұрын
Thank you Prof!!
@uniquegirl1094
Жыл бұрын
Detailed step by step guide on qPCR analysis, great video, thank you so much for sharing!
@mduduzingwane
Жыл бұрын
Great simple and straight to the point!
@momankhan6972
4 ай бұрын
Really Appreciating
@rosemarysollars2038
Жыл бұрын
This video is so informative; I have learned a lot. Thank you for putting this together ❤
@zachariaegbunu5350
Жыл бұрын
very wonderful teacher, so fulfil and hope for more of this
@hadisfirouzpour3150
6 ай бұрын
well done, it was fantastic, I love the way you mentioned the details and cautions, truly thanks for that
@spartan_gh
Жыл бұрын
I wish I could like this video a million times,Dr you did a massive job.Thank you😍
@joshuastephenkingsly
Жыл бұрын
Thanks for this wonderful tutorial!
@Miss.Thrillernightmare
10 ай бұрын
This video is very informative. Thank you so much for Explaining the PCR. I had no idea what PCR was! Your video is very helpful!!!
@YaredDDesta
Жыл бұрын
extremely important video and an excellent teacher. Thank you
@ruhidyusifov5106
Жыл бұрын
Indeed helpful
@nangirostephenomega1648
11 ай бұрын
It's been so informative and thanks for doing this video Dr.
@hassanmushtaq2220
Жыл бұрын
Wow. Amazing
@SuleimanNasiru-ot3jy
Жыл бұрын
thank you Dr. Lexa the demonstration is simple for beginners like me, I quite enjoy the video
@andrewafanasyev8860
Ай бұрын
A High quality video
@dorcasoluwatosin337
Жыл бұрын
This is a really informative video! Well done.... Thank you very much!
@user-me7ob4wq9y
Жыл бұрын
it was greattt thank youuuu
@DailyDrivenDeathTraps
3 жыл бұрын
Good job Hannah and Dr. Lexa!
@MANOUCHKA13
9 ай бұрын
Thank you very much for this great video. Love from Tunisia 🙏
@itforrealityshorts
Жыл бұрын
This video is so informative; I have learned a lot. Thank you for putting this together ❤ In the preparation of the standard, did you add 5 micro liters from the 10:1 tube to the 10:0 tube? I am a bit lost there.
@user-kk3xd6jr6z
3 ай бұрын
wow i love this contenent. !!!
@cerenertekin4693
5 ай бұрын
Wow, this is a great video. I’m not only talking about qPCR but in general lab work. Also amazing and clear explanations and suggestions. It was really interesting and I actually enjoyed it a lot. Thank you so much!!!
@prinsaregmi8566
Жыл бұрын
Thank you. You are life saver. 🙏
@ummuhaunijamalmohamed5497
Жыл бұрын
Thanks very good explanation along with the work
@BryanDellomos-zy1jl
Жыл бұрын
Thank you so much. Indeed, very informative.
@maalyaldoma6982
Ай бұрын
I liked the way you were working and the degree of organization you have . I would love to have a chance to be able to work with you, as i think you would be a great mentor for me . thanks in advance.
@JayaPrakash-tq6iy
17 күн бұрын
nice madam .. very informative
@michaelmaldonado1133
Жыл бұрын
me gusto mucho
@dr.hanifurrahman8183
Жыл бұрын
Great effort dr. Lexa, highly appreciated your efforts in this regard.
@igorbuzzatto
Жыл бұрын
The lab safety/good manners tips were terrific!
@xx-kb7lv
Жыл бұрын
Thanks for your sharing.It's very helpful
@jonathana9236
Жыл бұрын
This was helpful and very informative. thumb's up
@jonathana9236
Жыл бұрын
Is there a similar video preforming a gc-ms ?
@michaelgirma6838
Жыл бұрын
This is a great full overview of qPCR. Thank you, Lexa.
@swhyuni2136
Жыл бұрын
Thank you Dr. Lexa. I enjoyed your video, its clear and informative . How about nested PCR?
@aarpitabaa7339
11 ай бұрын
It was really greate.. But can u provide the softcopy of the information sheet you used in the video.
@ashikurrahman3307
3 ай бұрын
Really Easy and very Realistic.
@Nathelie
6 ай бұрын
Thank you for the video. But why didn`t you use gloves at the beginning?
@alexand4003
Жыл бұрын
Best video on KZitem.
@edenc5967
Жыл бұрын
Dude this is such a good video holy shit
@cutychandanacastle19
Жыл бұрын
Thank you sooo much mam for beautiful detailed explanation.😃😇😊
@JamesBond-ks2en
6 ай бұрын
Thankyou .Madam..it was a great real time experience with your beautifully explaination in simple way..
@junjiechen3843
Жыл бұрын
这就是专业
@muhammaddzariyat5302
5 ай бұрын
sometimes when i take like 0.5ul dna or 12ul mix there will be always leftover liquid in the tip that doesnt want to come out. is it because my micropipette is not good anymore or it just the way i use it is not proper?
@dumi2025
Жыл бұрын
Do you have any spots for hiring?
@sadiahaquekhan6003
7 ай бұрын
Dear Madam, pls accept my gratitudes. you are an an gel for me.I am struggling with qPCR and did not know how to start! can you please tell me the paper work you did for your lab pcr, how can I set mine for my lab?
@nasirofficials
2 ай бұрын
Hi, madam I watched this video and really understand your explanation. Please do a favor, I need video like this for agrobacterium infiltration in tobacco or etc. Thank you
@probablyabbas
11 ай бұрын
Thank you so much madam for this detailed and in depth video.
@marciamcfarlane3013
8 ай бұрын
Great. Learned a lot.
@sandeepsahota1484
5 ай бұрын
Can someone explain the dilution series she did I am lost by the math on those for this video.
@revathitathineni4938
Жыл бұрын
Dear madam nice over view on Real time PCR. The primers used in real time PCR can be used in normal PCR without probe
@HenryLetting
Жыл бұрын
💯
@debabratasenapati9845
Жыл бұрын
pls send some some sheet if possible because sheet shown in this video are not properly visible. thanking you.
@YouthInclusiveness
Жыл бұрын
What is the differences between qPCR and RT PCR?
@VinayHallurmicrobiology
2 жыл бұрын
Is it absolutely necessary to linearise plasmids before RTPCR?
@aadhithyanbabu
2 жыл бұрын
Yes , I think so.
@myxpony9728
5 ай бұрын
Thank you so much!
@MahnoorjamilJamil-nd5gw
9 ай бұрын
Well done description fully understood
@opokuderrick4931
3 ай бұрын
Nice video but can't you use the autoclave machine to sterilize the tools
@ajeetkumaryadav3546
Жыл бұрын
Thanks Ma'am 🙏😊
@evertjor
4 ай бұрын
Great video!😆
@banchnegn1880
7 ай бұрын
Great job! ma'am thanks
@achourwael6980
7 ай бұрын
Hello, could you please give us the information in the technical shit the paper contening all information about hte volume of each component thanks
@ashenafilakew7098
4 ай бұрын
Thankyou Very much!
@samaasameh4119
Жыл бұрын
Many thanks to you, doctor. I wonder if you generously explain how I can get the " working paper" 👍🌹
@beinghuman4457
5 ай бұрын
can anyone help mee pleasee... i want to separate mRNA(bax gene) from rat brain cells...and later wana do rt pcr to check bax expression level.. how will i know that the RNA which i separated is BAX...because there are many other mRNAs present in that cell.. please clear my confusion
@RiDankulous
3 жыл бұрын
I watch Twiv which is a virology podcast and they mention the use of PCR for testing of all kinds of viruses including SARS.
@amitjai20
Ай бұрын
Hi Dr. Lexa, can I get a copy of the Paperwork template?
@bioworld7565
8 ай бұрын
best video ever seen
@Amarjeet-qo8qs
Жыл бұрын
Great videoo maaam.
@muhammadpervaiz8347
Жыл бұрын
Good explanation , keep it up , Plzzz told about optimisation and cDNa dilution also
@lxm1117
Жыл бұрын
why no gloves?
@ugochukwuobi3513
3 ай бұрын
She did not glove up during instrumentation however she did during the process
@dr.vikrambhoomi-scientist
Жыл бұрын
excellent explanation madam
@Phytopathology
11 ай бұрын
Great Job!, thanks. I wonder where to get/prepare the starting materials for the standard curve. How to know the number of copies of my plasmid in the sample. Is this a linear DNA plasmid or RNA transcript?
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