How to performe Reticulocyte count stain procedure
For manual reticulocyte counts,
whole blood is mixed with a supravital dye such as new methylene blue and blood smears are prepared.
The dye causes clumping and staining of residual nucleic acid present in immature cells.
The stained cells (reticulocytes) are counted as a percentage of total red blood cells.
Staining Protocol
1. Combine 3 drops of completely mixed blood with 2 drops of Reticulocyte Stain in a glass test tube and mix well.
2. Allow mixture to stand for 15 minutes at room temperature or incubate mixture at 37° C for 15 minutes.
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