Great video, my college biochem professor is making us watch this video for lab today! I'm glad you're getting exposure and views!! Great explanation and visuals.
@animatedbiologywitharpan
3 жыл бұрын
Thanks man ...please share among friends and help me to reach big audience
@lmtrevino7
3 жыл бұрын
@@animatedbiologywitharpan You made an SDS gel and you forgot to add SDS in your gel mix. You realized this after you loaded your protein on the gel and began running it. What will your results look like? How will the protein migrate in the gel in the absence of SDS?
@eyalpaz6014
4 жыл бұрын
Excelent video. But I think there is a small mistake when you talk about Glycine. Glycine pI is 6.06, so at pH=6.8 it will have a weak negative charge and not a positive charge. If it had a positive charge it would not run into the gel, but go the opposite way and exit from above.
@hollygriffiths9719
3 жыл бұрын
Thanks for this comment, makes this video somewhat comprehendable... Cant believe I pay for a lecturer to direct me to videos like this that aren't even fully correct!!
@MannansElearning2021
3 жыл бұрын
Amino acid pKa1 pKa2 pI Glycine 2.34 9.60 5.97
@hola-lw1bi
2 жыл бұрын
@@MannansElearning2021 why does the pka carboxylic acid change per molecule?
@kaylanyoungman5517
Жыл бұрын
Very well explained with lots of information; thank you sir.
@animatedbiologywitharpan
Жыл бұрын
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
@neelamanna112
Жыл бұрын
Amazing..thanks a lot for wonderful animation😊😊
@animatedbiologywitharpan
Жыл бұрын
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
@bhaswatichakraborty9245
4 жыл бұрын
Such an informative and amazing video. Such visuals will definitely help the learners to grasp the topics easily. Much grateful! Hope to see such amazing content in the future as well. 😍
@animatedbiologywitharpan
4 жыл бұрын
Please share among your friends and help me to reach big audience
@plsfia4363
2 жыл бұрын
May I ask why the ionic strength necessary in the conduct of SDS-PAGE?
@sristi_2360
3 жыл бұрын
Could you please say that how can we experimentally analyse whether a single band represents a single protein or two different proteins with same molecular weight.?
@animatedbiologywitharpan
3 жыл бұрын
Spend only 4 minutes to get this answer kzitem.info/news/bejne/p36syKCdbGVpepw
@sristi_2360
3 жыл бұрын
@@animatedbiologywitharpan hey thank you so much. Btw, apart from 2D , can we use western blotting rather with the help of antibodies specific to my desired protein?
@n-sh2014
3 жыл бұрын
Fabulous! Thanks a million.
@animatedbiologywitharpan
3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@moviemania9726
3 жыл бұрын
Great explanation
@animatedbiologywitharpan
3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@cardiacmyxoma4073
3 жыл бұрын
Thank you so much!
@animatedbiologywitharpan
3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@khaledalharmoudi3350
4 жыл бұрын
Thank you. it helped a lot.
@animatedbiologywitharpan
4 жыл бұрын
Please sharee Among your friends
@yumabaker9850
3 жыл бұрын
Amazing! thank you.
@animatedbiologywitharpan
3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@sreeram6416
11 ай бұрын
Why specifically 5% is used in stacking gel
@animatedbiologywitharpan
11 ай бұрын
Because it has quite big pore size
@nikitampmaurya
3 жыл бұрын
Thank you!
@animatedbiologywitharpan
3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@nikitampmaurya
3 жыл бұрын
Our professor introduced us to your Channel. 😊
@animatedbiologywitharpan
3 жыл бұрын
@@nikitampmaurya wow ...so nice to hear that....please share with your friends and help me to reach out big audience.
@jinx2873
2 жыл бұрын
thank you
@animatedbiologywitharpan
2 жыл бұрын
Please share my channel link with your friends and help me to reach big audience...love from India
@raymondshine4774
2 жыл бұрын
It would have been better if you had put the references on each slides. That could be easier for us to find more infos
@animatedbiologywitharpan
2 жыл бұрын
Thanks for the feedback, I will incorporate this in future
@rahulmohanty4008
4 жыл бұрын
Loved it
@animatedbiologywitharpan
4 жыл бұрын
Please share among friends and help me to reach big audience
@fabianamorales2410
3 жыл бұрын
what are the applications of this technique?
@animatedbiologywitharpan
3 жыл бұрын
It can separate proteins based on molecular weight. It's the first step to perform a western blot.
@renupandey5597
3 жыл бұрын
Lovely
@animatedbiologywitharpan
3 жыл бұрын
Please share my channel link with your friends and help me to reach big audience
@ThiNKBiologyThiNKVISION
4 жыл бұрын
⭐Amazing Video Sir! If u don't mind, Can I translate this video to my native language to explain ? with respective credits to You. Thank You sir!
@animatedbiologywitharpan
4 жыл бұрын
Okk
@animatedbiologywitharpan
4 жыл бұрын
But share my channel link with your friends and help me to reach big audience
@ThiNKBiologyThiNKVISION
4 жыл бұрын
@@animatedbiologywitharpan of course sir! Thank you
@landrew3847
3 жыл бұрын
@@ThiNKBiologyThiNKVISION will you be translate it to english?
@ThiNKBiologyThiNKVISION
3 жыл бұрын
@@landrew3847 it's already in English
@user-jz4lm4oo7v
4 жыл бұрын
Why the protein becomes free?
@animatedbiologywitharpan
4 жыл бұрын
唐峰 I did not get the question ....could you please elaborate
@akanshasharma1643
4 жыл бұрын
Toooo goooodddd😘
@animatedbiologywitharpan
4 жыл бұрын
Please share among your friends and help me to reach big audience
@animatedbiologywitharpan
4 жыл бұрын
I am also available at Unaccademy
@supritadas1761
5 жыл бұрын
too good
@animatedbiologywitharpan
5 жыл бұрын
Share among friends and help me to reach big audience
@PritomAhad
3 жыл бұрын
R u bangladeshi?
@animatedbiologywitharpan
3 жыл бұрын
No, Indian
@AbeyPyrro
Жыл бұрын
You took 1 minute 16 seconds extra.
@animatedbiologywitharpan
Жыл бұрын
Sorry about that, i hope that the content was useful
@AbeyPyrro
Жыл бұрын
@@animatedbiologywitharpan was joking very well explained
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