No matter how much time i study in one topic, how many videos i watch on it , i need your video at any cost for my clear understanding and mental satisfaction... Thank you
@shomusbiologyofficial
3 жыл бұрын
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
@firewfufa6092
Жыл бұрын
I love your lectures. you are the reason why I got an A in molecular pathology. I really appreciate your help!!
@shomusbiologyofficial
Жыл бұрын
I am glad
@vishnushri4671
4 жыл бұрын
Teaching is not merely a money earning divice but it is astrugle and life mission. Without ambicues teaching touch souls.thanks.
@shomusbiologyofficial
4 жыл бұрын
Thank you. Glad you liked my lectures
@Gaeforhae
10 ай бұрын
Thankyou so much sir , our lecturer teaches us like we’ve already gone through the topic 10 times , he never clears basics and only goes on his lecture saying you already know you although know If it wasn’t for you I couldn’t have passed
@shomusbiologyofficial
10 ай бұрын
Glad to hear that you're getting benefit from my lectures. Please subscribe and share
@sciencenerd752
4 жыл бұрын
quarantine feels better with learning. thanks for your videos. :)
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
@ne6761
3 жыл бұрын
I am a university student in America and they way you explain things most of the best professors in my school cant explain it the you do! I would listen to them for hours and not know what they are talking about. But with you, I just cant not understand it!!!
@vrindapatel4091
4 жыл бұрын
You are amazing! You have been saving me throughout my undergraduate years. Thank you so much for everything you do for students all around the world. :)
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
@vrindapatel4091
4 жыл бұрын
of course! Even my American friends love your channel!
@mr.underhill5332
3 жыл бұрын
@@vrindapatel4091 SAT or GRE ?
@mohithinsu4017
4 жыл бұрын
Yesterday my teacher expalined this topic but i am confused many time so just search about electrophoresis and your video is found......thank you for helping to understanding in this topic
@shomusbiologyofficial
4 жыл бұрын
Glad to hear that you're getting benefit from my lectures
@nilaysaha1835
3 жыл бұрын
Thank you very much Sir...these two videos are more than enough to clear our confusion on gel electrophoresis..I wish to know more about Gradient Gel Electrophoresis from you...also watched your lecture on 2D gel electrophoresis...that was also very much interesting...Honestly speaking Sir...you are an Encyclopedia to us...on all these topics on Biology
@shomusbiologyofficial
3 жыл бұрын
Thank you so much for appreciating my efforts. You are a loyal subscriber. Keep supporting us.
@mohd_athar123
4 жыл бұрын
Excellent sir. Ur teaching way is amazing.I was in search of like this way to reach,now my thisrt comes to end to watch this video
@shomusbiologyofficial
4 жыл бұрын
Glad to hear that you're getting benefit from my lectures
@tammanaakhther8177
4 жыл бұрын
Thanku sir .... Not only i but every one pray for u... becoz lot of students gain more nd more knowledge through ur video that iz most helpful for our furture .the most thing iz that lot of students that are poor like me can pass their exams easily without any problem
@shomusbiologyofficial
4 жыл бұрын
Glad to hear that you're getting benefit from my lectures. Please subscribe and share
@tammanaakhther8177
4 жыл бұрын
Ok sir
@elisamayondi4145
3 жыл бұрын
I love your lecturers. You really simplify things
@shomusbiologyofficial
3 жыл бұрын
Thank you so much for appreciating my efforts
@krishnendumukherjee7633
4 жыл бұрын
Nice dress,looking always active and smart! Nice teacher for basic foundation of life science! 🙏
@ahmetsezer7591
4 жыл бұрын
May I ask you something I've watched your many videos and my question is how did you learn so much things . I wish I were like you
@shomusbiologyofficial
4 жыл бұрын
Thank you so much for appreciating my efforts. I wish I never stop learning
@shaliniramesh3406
2 жыл бұрын
Hai sir After watching ur lecturings sometimes without knowing myself, my eyes got filled with tears, equally am learning and feeling ur lecturings🤗 Thank you my Bhrahma 🙏♥️♥️
@shomusbiologyofficial
2 жыл бұрын
Thank you so much for appreciating my efforts. I am honored to see your comment.
@farwadaar588
4 жыл бұрын
Thank you so much sir..for ur efforts... keep it up...n love r style of presenting n discussing...these always save my time..n make my concept 99% clear..
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
@batoolgenetics2256
3 жыл бұрын
I wish I have the time to watch every single video in your channel Thank you for your amazing efforts
@shomusbiologyofficial
3 жыл бұрын
Glad to hear that you're getting benefit from my lectures. Please subscribe and share
@parshribailung1830
4 жыл бұрын
U r too good sir.....I saw ur all videos and also told my frnds that they watch ur videos😊😊
@shomusbiologyofficial
4 жыл бұрын
Thank you. Glad you liked my lectures
@atharvakulkarni8818
4 жыл бұрын
Sir i like your videos so much.I like thid one too.....i was unable to understand SDS -PAGE . I search for book too but did not find out.but your video helped mee alot!....pls also make video on detailed applications of modern technquies.....it will be so kuch helpful.🙂🙂🙂
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
@jayeetachowdhury970
4 жыл бұрын
Sir this vedio is very helpful for me... I hope you'll make a vedio on 2D Gel electrophoresis soon.. 🙂
@shomusbiologyofficial
4 жыл бұрын
Sure
@karthikajinivarna4215
4 жыл бұрын
It's very helpful to my undergraduate studies. Thank u so much sir
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
@ruksanakhan4989
4 жыл бұрын
U taught way better than my teacher...👍👍👍👍
@shomusbiologyofficial
4 жыл бұрын
Thank you. Glad you liked my lectures
@kavyap4453
4 жыл бұрын
Thank you for making my concept on sds-page very clear 😃👍👌
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
@deekshabheemireddy7180
4 жыл бұрын
Great sir, Iam feeling confident after learning subject from u
@shomusbiologyofficial
4 жыл бұрын
You're welcome
@rudradeephazra7355
Жыл бұрын
I am a mpharm pharmacology student.and your videos are helpful.
@shomusbiologyofficial
Жыл бұрын
You're welcome
@adarshkg1573
3 жыл бұрын
Lots of respect for ur knowledge n teaching skill ❤️
@shomusbiologyofficial
3 жыл бұрын
Thank you so much for appreciating my efforts
@interestingandinformationcombo
Жыл бұрын
very well explained lecture.... I must say you are a very brilliant teacher
@paramedical8133
4 жыл бұрын
Thanks sir..for explaining each and every key points🤗
@shomusbiologyofficial
4 жыл бұрын
You're welcome
@raenashaikh3377
2 жыл бұрын
This was really detailed and helpful... thanks a lot sir!!
@shomusbiologyofficial
2 жыл бұрын
You're welcome
@mahimakhatri6167
2 жыл бұрын
thank you for your valuable lecture sir it helps us a lot
@shomusbiologyofficial
2 жыл бұрын
You're welcome
@khadijatulkubra8343
3 жыл бұрын
Thank you so much sir, I am extremely thank ful to you, May ALLAH bless you with infinite happiness
@shomusbiologyofficial
3 жыл бұрын
Thank you so much for appreciating my efforts
@dharshanpriya5726
4 жыл бұрын
Respected sir, I request u to upload videos regarding drug discovery technologies and strategies and target validation from genomic and proteomics
@shomusbiologyofficial
4 жыл бұрын
I will consider it. Thank you
@ruwanihettiarachchi305
4 жыл бұрын
Thank you dear Sir! Well explained everything 👍
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
@Falakshorts751
3 жыл бұрын
Best way to teach
@tumpahazra5265
2 жыл бұрын
Thank u sir It is really helpful..
@shomusbiologyofficial
2 жыл бұрын
You're welcome
@ZeroSectionStyle
Жыл бұрын
Hey! Always thankful for your stuff, incredibly educative. I have a question. Ive been studying 2DGE lately, and my professor always stresses that you need to prepare the sample for the MW based separation with iodoacetamide to remove S-S bonds, but this seems to do the same thing as B-mercaptoethanol, does anyone know why doing both of these things is important, and why Iodoacetamide does not suffice on its own? Thanks!
@sudipakar2996
3 жыл бұрын
Thanks for saving our life❤️
@gershonmabas3762
3 жыл бұрын
Thanks a lot man, you are a great teacher. This is now really clear.
@shomusbiologyofficial
3 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
@jessychelsia5756
4 жыл бұрын
Sir ur awesome in teaching
@shomusbiologyofficial
4 жыл бұрын
Thank you
@trex-pubgmobile5390
2 жыл бұрын
Wow sir...🔥 awesome explanation
@shomusbiologyofficial
2 жыл бұрын
Thank you so much for appreciating my efforts
@wideworldofscience1721
4 жыл бұрын
Great method. Of explanation
@shomusbiologyofficial
4 жыл бұрын
Thank you
@shabanabegum6346
4 жыл бұрын
Superb 😀 by the way with explanation ur also looking nice sir
@shomusbiologyofficial
4 жыл бұрын
Thank you
@Jude_Ifechukwu
4 жыл бұрын
These guy done fresh
@mdrezoanulislamfahim1268
3 жыл бұрын
Thank you verry much ❤️❤️ from Bangladesh
@M_5787
2 жыл бұрын
Thankyou so much sir 😊🙏
@shomusbiologyofficial
2 жыл бұрын
You're welcome
@vasantharumugam602
4 жыл бұрын
very nice explanation sir thank you so much... Sir can I say this video about one-dimensional gel electrophoresis
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
@rajlaxmiprasad7087
4 жыл бұрын
Respect for u
@shomusbiologyofficial
4 жыл бұрын
Thank you
@divyasree3329
3 жыл бұрын
Well understood somu sir
@shomusbiologyofficial
3 жыл бұрын
Thank you
@bindusirishanallamalli3228
3 жыл бұрын
Thanks bro
@arnabdatta6971
4 жыл бұрын
thankyou sir for this video
@shomusbiologyofficial
4 жыл бұрын
You're welcome
@FatmaAli-tb8tq
2 жыл бұрын
Thank you but can you please state the reason behind using separate gels (stacking and resolving)?
@namratasingh4161
4 жыл бұрын
Nice explained this topic. Sir please suggest some books for research methodology if possible
@shomusbiologyofficial
4 жыл бұрын
Coming soon
@namratasingh4161
4 жыл бұрын
Thank you so much
@lisali2251
4 жыл бұрын
Thnku sir for your great effort towards us , u make us understand many things , but sir I am really confused to follow which video of shomus biology , means this one or one of your old video , can u plz recommend
@shomusbiologyofficial
4 жыл бұрын
New ones
@lisali2251
4 жыл бұрын
@@shomusbiologyofficial ok sir , thnku a lot
@tanushreeghosh6220
Жыл бұрын
Thanks sir ....
@shomusbiologyofficial
Жыл бұрын
You're welcome
@priyobratasinha7300
4 жыл бұрын
2.5 mAmp current is required in case of semidry transfer. Not in case of SDS-PAGE. Rather after SDS-PAGE the 2.5 mAmp current is used to transfer the proteins from gel to the nitrocellulose or PVDF membrane in case of semidry transfer process only.
@abhishekbhandary3693
2 жыл бұрын
Sir I had one doubt In SDS page all the proteins get negative charge from the SDS so that they can easily move towards anode, but in native page there is no charge donator like SDS then how positively charged proteins gets seperated?
@Amitkumar-ro3re
3 жыл бұрын
Just awesome..
@shomusbiologyofficial
3 жыл бұрын
Thank you
@anindita2169
4 жыл бұрын
in sds two types of gel are present one is stacking gel another is resolving gel. it would be much more of help if you have included this as well
@srividhyanarayanan7337
2 жыл бұрын
I was looking for it actually
@namjoonpark5309
4 жыл бұрын
Thank you so much
@shomusbiologyofficial
4 жыл бұрын
You're welcome
@vikasmurtadak8031
4 жыл бұрын
Nice teaching sir.
@shomusbiologyofficial
4 жыл бұрын
You're welcome
@qaisarsbiology5267
4 жыл бұрын
Love you sir
@shomusbiologyofficial
4 жыл бұрын
Thank you
@anshulrana5614
Жыл бұрын
Thanks
@shomusbiologyofficial
Жыл бұрын
You're welcome
@nknk-xo2qx
4 жыл бұрын
Sir plz as soon as possible make vid on gradient gel
@shomusbiologyofficial
4 жыл бұрын
Okay
@tanveerhussain3501
3 жыл бұрын
Amazing super cool !! LOVE from Pakistan🤩🤩
@shomusbiologyofficial
3 жыл бұрын
Thank you so much for appreciating my efforts
@cronosn_n9902
2 жыл бұрын
Tus vídeos son geniales puedo entender a la perfección sólo que por favor podrías agregarle subtítulos en español n.n!
@amogh7224
2 жыл бұрын
learn English its a global language
@tammanaakhther8177
4 жыл бұрын
Great sir....
@shomusbiologyofficial
4 жыл бұрын
You're welcome
@tammanaakhther8177
4 жыл бұрын
Thanku sir
@AlaaAhmed-sn8lk
3 жыл бұрын
Thank you❤️❤️
@shomusbiologyofficial
3 жыл бұрын
You're welcome
@AlaaAhmed-sn8lk
3 жыл бұрын
@@shomusbiologyofficial ❤️❤️❤️
@priyankasahoo3087
2 жыл бұрын
Can you please make videos on molecular biology experiments? Such as DNA isolation, protein estimation, protein purification
@shomusbiologyofficial
2 жыл бұрын
Okay
@ImranAhmad-df3mi
4 жыл бұрын
Nyc sir very helpful 😊
@shomusbiologyofficial
4 жыл бұрын
You're welcome
@lavleshmishra8846
4 жыл бұрын
Helpful 😊
@shomusbiologyofficial
4 жыл бұрын
You're welcome
@shivangi_1223
4 жыл бұрын
Well explained
@shomusbiologyofficial
4 жыл бұрын
Thank you
@Gatoradeia
4 жыл бұрын
Guys let's support shomu's biology
@shomusbiologyofficial
4 жыл бұрын
Thank you
@ammarahmehak9986
4 жыл бұрын
Sir plz refer any molecular book related to techniques e.g electropheris, sds, fish qrt pcr hybridization Bloating pcr etc plzzzzzzzzzz told the author name of that book your lecture is good thanx keep it up
@shomusbiologyofficial
4 жыл бұрын
Thank you. Glad you liked my lectures
@Falakshorts751
3 жыл бұрын
Thank u sir
@SambitPatra1
4 жыл бұрын
SDS attach to which amino acids of protein??
@pabitradebnath1917
2 жыл бұрын
Function of SDS is now clear.
@shomusbiologyofficial
2 жыл бұрын
You're welcome
@shradhamahendra3958
4 жыл бұрын
Your videos are good but not complete information is provided
@Falakshorts751
3 жыл бұрын
Best video
@ahelimukherjee3777
4 жыл бұрын
Can you please discuss on the mathematical problems based on SDS Page and ESI-MS? Like the ones asking for molecular weight of the protein?
@Wgh552
4 жыл бұрын
Thanku sir s much
@shomusbiologyofficial
4 жыл бұрын
You're welcome
@nikkipatel3199
3 жыл бұрын
Please make a video on immune electrophoresis
@shomusbiologyofficial
3 жыл бұрын
Okay
@bscool123
3 жыл бұрын
Separation take place on the basis of, because all particle have approximately the different charges , and different masses ???? Please tell me
@vidushisoam1395
2 жыл бұрын
Sir, you have not told about the stacking and separating gel difference..
@tammanaakhther8177
4 жыл бұрын
Sir can u plxx....arrange a class on ultra centrifugation
@shomusbiologyofficial
4 жыл бұрын
Coming soon
@tammanaakhther8177
4 жыл бұрын
Sir my test coming soon ....can u arrange plzz before 1june
@tammanaakhther8177
4 жыл бұрын
Sir but plzz it should be in urdu language and on white board ...i mean as u given ur SDS topic on white board
@idealexotic4072
4 жыл бұрын
I have one question what is denaturing polyacrylamide gel electrophoresis and how is this different from native page or SDS page?
@shomusbiologyofficial
4 жыл бұрын
In native gel, we don't denatured the protein. It moves as it is in structure and in denatured gel, we denatured proteins and then allow it to migrate in gel
@prajaktaharmalkar7952
2 жыл бұрын
SDS-PAGE is also a discontinuous gel system made up of two different pH gels called stacking gel of pH 6.8 and separating gel of pH 8.8 which also differ in its pore size. You have not mentioned this important point
@shomusbiologyofficial
2 жыл бұрын
Thank you for mentioning
@prajaktaharmalkar7952
2 жыл бұрын
@@shomusbiologyofficial Thank you sir. Also thank you for such easy and well explained videos.
@ankitaroy7153
4 жыл бұрын
Sir really really thank you so much 😊 Sir mujhe 1 Question puchhna h SDS-PAGE m do Ph Q lete h Resolving Gel & Stacking Gel k lie ? Please sir please please reply with d answer. I have to answer this Please sir
@paramedical8133
4 жыл бұрын
Sir,why we need to separate the protein...clinical significance???
@sakshisingh8954
Жыл бұрын
Is SDS-PAGE is horizontal type of electrophoresis??
@abhijitaher1515
2 жыл бұрын
Also I have one more question that as we use tris glycine buffer as running gel in sds. The glycine enters the gel and help in lining of the proteins before entering the resolving gel. Is it possible to run sds if we don't use glycine.
@mahakehail8425
3 жыл бұрын
Can we separate RNA by Agaros gel also? And if we have mixture of DNA snd mixture of RNA in gel, who will being faster? DNA or RNA Or we not compirsion DNA and RNA togather? Just DNA size it self and RNA itself?
@souvikhajra4213
Жыл бұрын
Sir where Resolving gel and stacking gel?
@rakshajain43
2 жыл бұрын
How many polypeptide bands detected on gel, if we electrophorised secretory IgA on SDS-PAGE under reduced and denaturing conditions. Sir, plz answer urgently 😔😔😔😔
@rashiyadav7017
3 жыл бұрын
Great video! I have a question- in one of my experiment, I noticed that a diner protein (44kda) was migrating slower and was found at 50kd. This dimer has positively charged amino acids. Can you please tell me what could be the possible reason for slow migration of this protein?
@krupalimakwana5941
Жыл бұрын
What is stacking and separating gels in SDS page ???
@tejureddy5474
3 жыл бұрын
How much of voltage is sufficient to run an SDS PAGE to get a protein band of molecular weight 27kDa
@neetladder
3 жыл бұрын
Why we get four bands for BSA in SDS PAGE? If you have an proper explanation then please help me?
@rohitsutar4981
4 жыл бұрын
Why SDS give 2 band in antibody separation?? Plz ans...
@jhajiya9297
4 жыл бұрын
Namaste sir
@shomusbiologyofficial
4 жыл бұрын
Namastey
@jhajiya9297
4 жыл бұрын
Thank you sir ji
@gayathrigaganan202
4 жыл бұрын
Sir, which amino acid interfere with the SDS page?
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