I have a "chicken or egg" type question! where does the uniprot database come from? if it is the mass spectrometer that gives the sequence information, does the uniprot database derive from mass spec data? I also have another question; is uniprot different from a spectral library database for data independent acquisitions?
@shimonaahlawat5506
2 жыл бұрын
Which software you have used for matching the fragment ion of peptide?
@mattpadula837
2 жыл бұрын
They are mainly manually annotated. Otherwise, I have used Peaks Studio since v4.5 (~10+ years).
@leoalmeida2583
2 жыл бұрын
Can you please name that 2010 paper?
@MatthewPadula
2 жыл бұрын
Can you be more specific?
@leoalmeida2583
2 жыл бұрын
@@MatthewPadula I wanted to read the paper at 13:20 but I could not find it on the internet.
@MatthewPadula
2 жыл бұрын
@@leoalmeida2583 Nesvizhskii, A. I. (2010). "A survey of computational methods and error rate estimation procedures for peptide and protein identification in shotgun proteomics." J Proteomics 73(11): 2092-2123.
@leoalmeida2583
2 жыл бұрын
@@MatthewPadula thank you very much. I started my journey into proteomics and your channel has been very useful.
@brookcommunitytheatre9368
2 жыл бұрын
@@leoalmeida2583 Excellent. Don't be afraid to ask more questions. Either here or via email.
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