Thank you so much for taking the time to make this video! This was the clearest way I've heard anyone describe site-directed mutagenesis
@skouibi
7 жыл бұрын
subscribed, i wish you were in my college....10 minutes of pure explanation without changing topics and adding irrelevant information. well done sir!
@emilyb3622
3 жыл бұрын
This video right here is a life saver. I read textbooks, papers and articles and nothing helped me understand SDM like this video.
@Angel101Cales15
7 жыл бұрын
This was by far the most clear video I have watched on site-directed mutagenesis! It simplified it down so anyone with a bit of science background can understand. Thank you! :)
@spareluck
8 жыл бұрын
Fantastic explanation. You truly have a unique talent for making the complex world of biochemistry so accessible and interesting.
@ShortenMonteCristo
8 жыл бұрын
I don't think I've ever watched a video by AK Lectures and not understood the concept thoroughly. I cannot say the same of other instructional videos (including Khan Academy, who I use extensively)
@AKLECTURES
8 жыл бұрын
Thanks Adam! Appreciate that
@nachinathan3846
6 жыл бұрын
So true!
@evorock
7 жыл бұрын
Yet again, another brilliant mini lecture. Seriously, you are helping me get well on the way to completing my degree. I used your courses to help me pass my Human Biology module last semester (one of my first second year courses) and you are proving invaluable in helping me with cell biology and biochemistry. If you ever come to the UK for a holiday please let me know because I owe you a pint or 6 (especially if I pass my degree using your material to finish with a good enough degree classification to enter teacher training). Thank you so much for these, they are some of the best bio lectures I've seen on you tube (as well as Khan Academy!)
@sanhitasaxena1542
5 жыл бұрын
There couldn't be a better way to explain it. Saved a lot of time, thanks a lot!
@sunggutetris8311
6 жыл бұрын
Explained in a thorough, but simple way. Perfect. Thank you for this amazing lecture.
@dr.carmenmmartinez789
6 жыл бұрын
I am a master student and This is the most well explained video I have ever seen in youtube.This video and all of the rest.Thanks
@Hugihugo
8 жыл бұрын
This is a portrayal of true didactic and rhetorical skill.
@somenerdyblonde
4 жыл бұрын
Really thorough and clear. Now I can plan my mutants! Thanks!
@WaverlyRose
7 жыл бұрын
Beautifully done! I send your videos to everyone! Thank you so much.
@wilmeraltenagroep6832
5 жыл бұрын
You didn't mention how to remove the parent strand, can you add one or more methods in the video describtion? Note that I'm totally proud at this video, because it is one of the best on youtube explaining site-directed mutagenesis.
@tornado100able
Жыл бұрын
i don't think it's necessary because the parent strand will be 1 between millions of modified strands after all PCR cycles. When using the plasmids for transformation, the positive clones obtained most likely will not receive the original parent strand
@Mos4lk
5 ай бұрын
It is important because the parent strand could be amplified in further PCR cycles as well, there are some ways to get rid of the parental strand. A common one is the use of the restriction enzyme DpnI which can only digest methylated DNA. Methylation is a form of DNA modification which is done naturally in organisms like E.coli where most of the plasmids come from. The newly synthesized strands are not methylated.
@suboh1
9 жыл бұрын
Well described! I've looked around many websites searching for a good video, and by far, you have the best explained Site-directed Mutagenesis description. Thank you so much for the time and effort. Keep up the great work you do.
@AKLECTURES
9 жыл бұрын
Mohammad Suboh Thanks! Will do :)
@ranahanna160
8 жыл бұрын
Amazing! Thorough and simply explained
@jeff-mv5up
7 жыл бұрын
you saved me for biochem lecture and now you're saving me for lab work i'll be doing
@ahmadmuezuddintajulros7914
4 жыл бұрын
The way you described it makes me understand even more
@zheidari001
7 жыл бұрын
thank you so much, your clips helped me to get through my degree, i don't know how to thank you
@anonymakeupstar1
8 жыл бұрын
your explanation is much better than my prof's, THANK YOU!!!
@karinamatos4253
3 жыл бұрын
He is a great teacher. I would never be bumped to go to a class of him.
I don't know how to thank you enough ! very helpful explanation.
@isadora13correa
6 жыл бұрын
You are the absolutely best!! I honestly wish you were my professor
@anarchosurgeon
6 жыл бұрын
This was an awesome video. I watched at 2.3 speed (lol) and still got everything because he is very clear
@blonine8668
6 жыл бұрын
The best video on the subject. Thank you!
@bellabarrow8791
3 жыл бұрын
Love AK Lectures! Again getting me through my degree! :-)
@havanaaa
9 жыл бұрын
Thank you! Got the knowledge which I have returned to my lecturers
@maheshwarimahi145
6 жыл бұрын
This is the best video ever !! Thank you!
@rhmmasayyed6123
6 жыл бұрын
i'm from egypt , and i want to say that you save me always before my exams .. really thanks ! you're the best teacher ever .✋
@Ninjaluke333
8 жыл бұрын
You are the best! :) I'm studying biotech at University level and these videos are perfect! :)
@AsherBrandt
5 жыл бұрын
Very well explained I need to do this process for my research. Thanks for the clear explanation.
@asdfghjkljillianne
2 жыл бұрын
Everytime he finishes explaining something its like revealing the trick in the magic trick
@minshuli2988
2 жыл бұрын
Love your lectures. it's really easy to understand!
@cynthiabernal4794
8 жыл бұрын
te amooo!!!!explicas super bien :) I hope you understand spanish! I'm watching you from Paraguay
@ziedkhedher9660
8 жыл бұрын
thank you!! it explains everything in a very simple way.
@malikbasharat3916
7 жыл бұрын
i seriously think...you are unique piece on the globe..bt i wonder y your videos get less views.they are worth of crosing million...may u live long and healthier
@bharathraj5862
5 жыл бұрын
Well said
@user-ut5lr7hd4n
Жыл бұрын
Great video! You have it perfectly structured
@taniyakhan1931
Жыл бұрын
You are such a good lecturer...and a life saver too 😭 thankyou so much 🌼
@philipw2388
5 жыл бұрын
Watched it once and got it! Keep up that awesome work :)
@yoloitsamixxx
3 жыл бұрын
the best videos ever honestly
@jesicasmit
3 ай бұрын
Thank you Andrey!
@TheCooldudenike
5 жыл бұрын
This video is GOLD!!!
@harmeetsingh7894
4 жыл бұрын
Thank you so much sir . You have explained the concept in very simple and interesting way .
@impossibleaquariums9975
5 ай бұрын
U guys need captions. Love it. ❤
@bajawabajawa3855
2 ай бұрын
After a jard struggle from almost every sourse finally this was fruitfull
@issaabujeries2912
4 жыл бұрын
Absolutely Phenomenal!! You should teach professors how to teach. Thank You!
@zainabkhalid7040
4 жыл бұрын
Best way of explaining lecture. It's help me a lot thank you for making this video
@ahmedal-salihi4528
Жыл бұрын
This was fantastic Andrey, well done, and thank you :)
@moonabutt9565
6 жыл бұрын
Fantastic. You are the best teacher.
@marouabouraiou613
5 жыл бұрын
wonderful explanation you are helping to get good marks in exames!! god bless you!!
@xXMyChEmxXxLoVeRXx
6 жыл бұрын
In primer design, is it necessary to put the point mutation in 3' or right in the middle? Im wondering if it would have an effect at all. Thank!
@ujjainimukherjee4328
2 жыл бұрын
Thank you so much! You are a superhero
@paneerchadda4375
4 жыл бұрын
Nice explanation Thanks you were a big help
@entedaralsaadi21
8 жыл бұрын
Thanks, very useful and comprehensive explanation
@medinlab141
3 жыл бұрын
Very clear! Thank you for the explanation
@TheZechariah
2 жыл бұрын
Beautifully explained by friend
@user-iz2yu4qo3v
4 жыл бұрын
It's great. You are the best teacher.
@human1000000
7 жыл бұрын
great job, very helpful video. thank you...
@adityakanjoor
8 жыл бұрын
beautifully explained.. the explanation is perfect... thank you so much .. :)
@mohamedbabiker6653
9 жыл бұрын
Tank you for this amazing lecture. There is something I couldn't understand which is wouldn't the bacteria repair the mismatched base.
@melanie8388
8 ай бұрын
Super helpful, thank you!
@Auntie_Joker
7 жыл бұрын
i'll be sitting for an exam tomorrow.. A huge thank you for this video! Just wanna ask, in step 3, what will happen to 'black' single stranded DNA molecule as the DNA polymerase and dNTPs are added into the mixture? I mean, it will probably elongate the black DNA molecule as well. Just to clarify this part. Thank you :)
@augustgene5732
5 жыл бұрын
Thank you, for this.
@jsufi1
7 жыл бұрын
thanks god for 1.5x speed but very clear explanation
@JongensJavaanse
6 жыл бұрын
In Step Number 3: I can make sure to only amplify the modified strand by only adding the accroding 5'-3' Primer (TCC...)! Right?
@md.moniruzzaman7944
Жыл бұрын
Thanks bro for your nice explanation
@rachelcurrry
3 жыл бұрын
so useful! thank you very much
@XY-yg1ci
4 ай бұрын
clear, with moderate speaking pace
@tahirtantary7438
3 жыл бұрын
Informative ❤️
@Shadow-bx8ri
4 жыл бұрын
You are amazing, sir...................
@AnkushSharma-zv5hv
4 жыл бұрын
THANK YOU A LOT
@savvy8268
2 жыл бұрын
Thanks so much!
@alejandrobustoscolas2938
2 жыл бұрын
Very clear!
@yashaswinisalian
5 жыл бұрын
Thanks for a great lecture! :) But just couldn't avoid noticing a misspelled word; 'mistached' instead of 'mismatched' (left bottom)!
@brycehart6480
5 жыл бұрын
At the end of step 2, won't there be a nick in the new strand of DNA because there is no DNA ligase? Also, step 3 shouldn't occur because DNA synthesis of another strand can't occur without a reverse strand primer that provides a 3' OH.
@tornado100able
Жыл бұрын
I think he forgot to include the antisense primer
@chakri620
4 жыл бұрын
will this reaction be done using a pcr or in a Eppendorf tube
@aboutstudies766
6 жыл бұрын
A great lecture Sir. Thank you sir
@IxNergal
9 жыл бұрын
Great!!! Thank you so much!!! Simple and easy!!
@AKLECTURES
9 жыл бұрын
Mario Morales you're welcome Mario :)
@auryogomes4320
5 жыл бұрын
very good. perfect
@mubasharkhan9795
8 жыл бұрын
beautifully explained .. Thank u sir :)
@Micro-life
6 жыл бұрын
All ur videos r really grt..thnks a lot sir...bt i apologz to say tht i didnt gt ur 3rd point..hw cn dna pol start replicatng daughtr strand wthout a primer?
@ot7dhariwal535
4 ай бұрын
Best Teacher Ever 🫡
@anirudhchendeliya1875
4 жыл бұрын
thankq so much sir love from INDIA
@oidualclaudi0
3 жыл бұрын
I don't understand how is the new DNA molecule circular again Does the polymerase make it circular or you're adding a thermostable ligase to bound the resulting DNA molecule and make it circular?
@sonan2187
6 жыл бұрын
Amazing !!!
@twigburst
7 жыл бұрын
Thanks a lot.
@casualcasual1234
3 жыл бұрын
May I ask how to separate the sense strand from the antisense strand in the first step?
@junczhang
8 жыл бұрын
thank you so much!
@user-xv5wt5sd8h
Ай бұрын
Thanks so much for making this video.I am a Chinese student who major in biology.I cannot always understand the auditory signs with my literate faulty.
@TuNguyen-yv7lv
3 жыл бұрын
If we do this in pcr reaction chamber how can we seal up this plasmid into the circle one
@delroymunashe3487
2 жыл бұрын
can someone assist me here, at exactly 8:31 he mentions that dna polymerase attaches at the 5' and starts synthesizing the template going in the 3' direction but i read somewhere that dna polymerase does not bind to the 5' site because it is INACTIVE, is this wrong?
@carolinegreen4771
7 жыл бұрын
I'm wondering what's the difference between #Site-Directed #Mutagenesis and #Site-Saturation Mutagenesis(I saw this on Creative BioMart)?
@chriscarbe1042
8 жыл бұрын
EXCELLENT!!
@quililank
8 жыл бұрын
Can you please do a lecture on primer extension?
@wunlawealth
9 жыл бұрын
thank you!
@bharathraj5862
5 жыл бұрын
Sir, what is the difference between the Site Directed Mutagenesis and Single Nucleotide Polymorphism? Please clarify
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