Hey Friends,
Transformation, Conjugation and Transduction are ways of bacteria to transfer genetic material horizontally. Transformation is the uptake of free genetic material. Conjugation is exchange of DNA via cell-cell contact and Transduction is gene transfer via a viral vector. In the lab, we use Heat-shock transformation to make bacteria competent to take up plasmid DNA or we use lentiviral transduction (Lentivirus) to integrate genes in the genome of eukaryotic cells.
Cheers
Henrik
References:
Bacterial Transformation:
www.thermofisher.com/de/de/ho...
www.ncbi.nlm.nih.gov/pmc/arti...
Transduction:
www.ncbi.nlm.nih.gov/pmc/arti...
www.nature.com/articles/280080a0
Bacterial Conjugation:
www.ncbi.nlm.nih.gov/books/NB...
Cool & Short Blog article:
blog.addgene.org/plasmids-101...
Chapters:
0:00 Introduction
0:54 Transformation
2:43 Transduction
4:09 Conjugation
Негізгі бет Ғылым және технология Transformation, Transduction and Conjugation (Horizontal Gene Transfer in Bacteria)
Пікірлер: 52