Trends of THC Accumulation in Flowers and Leaves Over Time
Professor DeBacco
Research Article
Richins, R. D., Rodriguez-Uribe, L., Lowe, K., Ferral, R., & O’Connell, M. A. (2018). Accumulation of bioactive metabolites in cultivated medical Cannabis. PloS one, 13(7), e0201119.
journals.plos....
Time course for accumulation of Δ9-THC in flowers of medicinal marijuana
Floral and leaf samples were collected from two strains, Sour Willie and Bohdi Tree at multiple intervals following the floral induction.
This plant material was collected from greenhouse grown plants. The accumulation of Δ9-THC in these samples was determined
Accumulation of Δ9-THC in organs of Sour Willie following floral induction
Δ9-THC levels in samples collected from Sour Willie (A, B) at days post-induction are represented.
Panel A report average (n = 3) Δ9-THC levels in floral samples (solid line) and leaf samples (dotted line).
Panel B report Δ9-THC levels in floral samples from the top of the plant (solid) or bottom of the plant (dotted line).
Accumulation of Δ9-THC in organs of Bohdi Tree following floral induction
Δ9-THC levels in samples collected from Bohdi Tree (C, D) at days post-induction are represented.
Panel C report average (n = 3) Δ9-THC levels in floral samples (solid line) and leaf samples (dotted line).
Panel D report Δ9-THC levels in floral samples from the top of the plant (solid) or bottom of the plant (dotted line).
Δ9-THC Content
The Δ9-THC content of the samples from both strains increased in the floral samples with increasing time post-induction
In leaf samples Δ9-THC levels decrease slightly during this same time period.
Δ9-THC Content Variability
Considerable variation in Δ9-THC content in the floral samples at each time point for collection was noted
Multiple flowers were collected from different parts of the plant to generate an average.
Δ9-THC Content Related to Location on Plant
Floral samples from the top or high portion of the plant have higher levels of Δ9-THC than floral samples from the lower portion of the plant.
Consistent Pattern of Accumulation Observed
This same pattern of accumulation was observed in two additional strains, increasing the biological replication of this observation.
The Δ9-THC content is highest in flowers in the upper third, lowest in the flowers from the lower third, and intermediate in flowers from the middle third of the plant.
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Title: Trends of THC Accumulation in Flowers and Leaves Over Time
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Time course for accumulation of delta-9-THC
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Delta-9-THC Content
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Delta-9-THC Variability
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Delta-9-THC Content Related to Location on Plant
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Consistent Pattern of Accumulation Observed
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