Diazotised sulfanilic acid reacts with conjugated bilirubin and gives purple color immediately (within 30 seconds)-DIRECT POSITIVE VAN DEN BERGH REACTION
Diazotised sulfanilic acid plus methanol(dissolving agent) reacts with unconjugated bilirubin and gives purple color normally within 30 minutes.-INDIRECT POSITIVE VAN DEN BERGH REACTION
If the serum contains both unconjugated and conjugated bilirubin in high concentrations, the purple color is produced immediately which is further intensified by the addition of alcohol.-BIPHASIC VAN DEN BERGH REACTION
Негізгі бет Van den Bergh reaction | Biochemistry | Types of Jaundice | Medcampus
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