This... this is an amazingly beautiful blot. Hard, black bars, no background noise, no unnecessary ladders. I think I'll just print it out and hang it on the whiteboard. You have my respect.
@ATARISING
11 жыл бұрын
Thank you for the tutorial. It was very helpful and informative and has given me a better understanding of the protocol. I will be running my first ever Western Blot in the next few days!!
@deekshamarwari7112
Жыл бұрын
Thank you so much for uploading this video 😊🙏
@CR-uu1rr
Жыл бұрын
Very detailed clear exhaustive you have taken away all my doubts . Spectacular step by step. Amazing
@zulkifleekuedo6177
5 жыл бұрын
Thank you so much. It is very clear and helpful WB video for me
@dragosgheorghe9350
10 жыл бұрын
Outstanding. From someone who tested hiv negative using eclia and just found out if positive it is recommended to use this technique. I am stunned, amazing work
@vidhyaanish6888
10 жыл бұрын
the video was very helpful and explained each steps very clearly.Thanks a lot.
@NovusBiologicals
10 жыл бұрын
Glad that you enjoyed it!
@frass99
5 жыл бұрын
A very beautiful blot.
@seshadri16
10 жыл бұрын
Very well explained, helped me understand the protocol much better !! Thanks for the video !!
@NovusBiologicals
10 жыл бұрын
Glad that it was helpful!
@khadijahm.m.7178
9 жыл бұрын
Really thank you. This video is really easy to understand. Thank you again.
@laylays_world16
10 жыл бұрын
This is a great video. Very informative to collaborate with my presentation on western blotting. Thanks!!!!
@Dnjeb
3 жыл бұрын
awesome. now im ready for my experiment next week
@mohamedabdellatif1489
11 жыл бұрын
Absolutely brilliant and very descriptive...thanks a million
@neurobiogirl
10 жыл бұрын
THANK YOU!! Very clear, concise and articulate.
@PatreeceCJYou
11 жыл бұрын
Thanks for posting this. Helps me with my exam! :)
@deekshamarwari7112
Жыл бұрын
We want more biological techniques vedioes in this manner.. please upload more videos of other techniques in detail 🎉😊🙏
@madeleinem9357
5 жыл бұрын
Thank you. So amazing!!
@NovusBiologicals
5 жыл бұрын
Glad that you enjoyed it!
@shahidkhanalvi9519
3 жыл бұрын
Thank you 😊
@MisterAlbertoPiano
10 жыл бұрын
Great video! Amazingly helpful and very well explained. One little question: In this case, what was the final ECL volume used on the last incubation? I know that it depends on the surface of the membrane (e.g. 0,1mL ECL/cm^2 aprox.) In my case I have a 7 X 8.5 cm membrane, so I just need 6 mL of ECL (following the manufacturer's equation). The problem is that I'm not very sure if 6 mL would cover the membrane in that type of Incubating box that you use (mine is extremely similar). What would you do in my case? You would increase the volume of ECL even is not needed or you would incubate just with 6 mL ECL. Thank you again for uploading this great video. It help us out a lot!
@NovusBiologicals
10 жыл бұрын
Glad that you enjoyed the video! In regards to your question, to be safe use 10 ml of ECL, this is what use. Excess ECL is not a problem. The key is to make sure the surface of the membrane stays wet and does not dry out. You can add the membrane to the ECL and rock the chamber back and forth to keep the surface wet. Hope that helps!
@MisterAlbertoPiano
10 жыл бұрын
***** Thank you very much for your quick response!! It will help a lot!
@BilalAhmed-vr8rq
3 жыл бұрын
Very well explained
@gaurangtrivedi3713
11 жыл бұрын
Simply amazing..!! Brilliant
@MonaraKaelle
4 жыл бұрын
Great, thank you!
@irenakavrakova3801
4 жыл бұрын
when we incubate with more then 1 Antibody do we have to block for 1 hour before every incubation
@mmst3620
8 жыл бұрын
Very helpful tutorial video, thanks
@nurture-nature...
6 жыл бұрын
very well explained.... thanks a lot....
@hassoeai
8 жыл бұрын
Great tutorial! I like the two chamber tray used in phase 3, membrane transfer. where did u get it from?
@MA-dq9uz
3 жыл бұрын
world
@iranrose2002
11 жыл бұрын
it was perfect i really appreciate
@tilovatulkhondaker
8 жыл бұрын
thank you, it was helpful
@nilsjohansson4222
3 жыл бұрын
when and where do you put copper stain?
@NovusBiologicals
3 жыл бұрын
Hi Nils, thank you for your question. The Copper stain is a reversible stain that, while not required, is an optional step. The Copper stain is used on the gel itself after gel electrophoresis to confirm the migration and separation of proteins in the gel. This step would be performed prior to the membrane transfer and would require destaining the gel before proceeding with protein transfer. Hope that answers your question!
@colombarillo9123
11 жыл бұрын
Thank you!! Page bookmarked.
@UNEARTHED36
8 жыл бұрын
Premade gel!? I have to make mine.
@bakhtawarkhan9547
5 жыл бұрын
Thank you
@reemalsereihi4705
4 жыл бұрын
amazzzzzzzing
@123superdeduper
11 жыл бұрын
This is cool
@helmimuhammad7801
6 жыл бұрын
GOOD
@TheRand20
2 жыл бұрын
hmm why is she putting her hand outside the hood and then back again without cleaning them ? if everything must be sterile, you should never stick your hand outside the hood.
@robertsullivan5450
7 жыл бұрын
How i manage to do all that at full concentration working inside a lab which is monitored the whole time with a obnoxious lab lady?
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