Shomu You are really genious. Your way of explanation is very nice and easy. I am also teacher and doctorate but you way of teaching is quite better than me. Respect for you
@shomusbiologyofficial
4 жыл бұрын
Thank you so much for appreciating my efforts
@normadicn5700
4 жыл бұрын
I agree completely. I was so confused reading textbook and don't need all this indepth knowledge but he explains it so nicely
@revocatusredemtus9701
3 жыл бұрын
thanks alot prof.shomu may GOD go before you on everything you expect, coz you have a good lecture
@sajidahussain7406
3 жыл бұрын
Great job sir..solving our problems in an easiest way.. jzakillah
@vaiyascould5823
Жыл бұрын
Sir, do you provide any notes
@shinarashid
6 жыл бұрын
Dear Shomu, I am a teacher and regularly listen to your videos to help me make students understand in an easy way. Ur excellent. God bless u.
@shomusbiologyofficial
6 жыл бұрын
Thank you very much for appreciating my efforts
@Munzz1097
3 жыл бұрын
You can directly ask them to see this. Because I'm student and he is our savior .
@janefatalukdar5014
4 жыл бұрын
I have watched all ur videos during my graduation, as m in my last semester. To be very honest, Sir your lectures help me a ton in making my foundation in microbiology. Keep doing great works. May God bless you. Always greateful 👏👏👏
@shomusbiologyofficial
4 жыл бұрын
Thank you so much for appreciating my efforts
@구름짱-g3l
8 жыл бұрын
Thank you so much for your lecture. I finially understand the material. I think You are really talanted at teaching! I hope you keep on posting these helpful videos😊😊
@aliakhan-fn3bm
6 жыл бұрын
Sir thank you so much for western blotting explanation. I have exam on 17oct n it will help me.meri dua h k Ap aise hi sabki help Karein or Apka shomu biology khub tarakki Kare Inshallah 🤗😊
@shomusbiologyofficial
6 жыл бұрын
Thank you so much for appreciating my efforts
@anjaligond8638
2 жыл бұрын
Sir u dont disappoint me when i want study any topic of biology i found each and every topic of ur lectures🙂
@shomusbiologyofficial
2 жыл бұрын
Thank you so much for appreciating my efforts
@RiyaRoy-ey5jv
Жыл бұрын
But I can't find autoradiography topic
@RiyaRoy-ey5jv
Жыл бұрын
I want to learn from him 😭😭
@adesinaomoloye7510
8 жыл бұрын
A very didactic and informative tutorials. You are doing great!
@shomusbiologyofficial
8 жыл бұрын
+adesina omoloye thank you. Glad it helped
@prithaghosh1890
8 жыл бұрын
excellent sir...I am verymuch impressed by the way you teach
@shomusbiologyofficial
8 жыл бұрын
+Pritha Ghosh thank you. Glad you liked my lectures
@andy9938-x6j
2 жыл бұрын
Sir you said when current passed from gel (-ve charged) to membrane (+ve charged) by by conventional we know current move from " +ve to -ve". Then how this imprinting is formed? And why the gel has to be negatively charged?
@GeneticsLessons
2 жыл бұрын
DNA is negatively charged and moves to the positive electrode.
@rogersevers123
5 жыл бұрын
Shomu this is an excellent tutorial on western blotting. I just read the methods section of a research paper on western blotting and didn't understand much but after watching ur video I can confidently say I understood everything in that method section of the journal now! Thank u!
@shomusbiologyofficial
5 жыл бұрын
Glad to hear that you are getting benefit from my videos
@emirsbio-chemistry4017
4 жыл бұрын
Because of you sir my concepts are very clear about the topics that I listened ur lectures.. you are really an awsm teacher..May Allah bless you with more Knowledge And Success..🙂
@shomusbiologyofficial
4 жыл бұрын
Thank you. Glad you liked my lectures
@fa5774
5 жыл бұрын
Thank you so much!! You are truly talented when it comes to teaching!! Everything is explained in such an understandable and basic manner! Keep it up😊😊😊
@KeepRowing247
4 жыл бұрын
Yeah Shomu I agree with all these comments, you are essentially a God among Gods.. Quite frankly, you are a savior.. i've been watching your videos ever since I began my biotechnology degree. Whenever I need extra explanation on any concept, I am sure to find a video of you doing the expert explanations.. Thank you!
@shomusbiologyofficial
4 жыл бұрын
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
@shivanithakur9788
Жыл бұрын
I have my interview for associate scientist in 2 hours and I’m listening to your videos..these are so helpful and I just want to say thank you for pouring your knowledge here. May God bless you with everything you desire❤
@shomusbiologyofficial
Жыл бұрын
Thank you so much for appreciating my efforts. All the best
@vansheekasingh2550
Жыл бұрын
Heyy did you get the job!
@meenurambe3143
6 жыл бұрын
I had my immunology exams sir I did it very well .. thanks to you. Love your teaching ❤️❤️❤️
@Lawrrenz
5 жыл бұрын
SDS-PAGE DOES NOT separate molecules by charge, only by polypeptide chain length (size) 5:04
@twinkle1548
2 жыл бұрын
Thank sooo soo much sir really have no words to explain for your hardwork keep it up, good will bless you forever and ever and ever❤❤❤❤❤❤❤🤗😊🙏
@shomusbiologyofficial
2 жыл бұрын
Thank you so much for appreciating my efforts
@jolen4542
3 жыл бұрын
I'm not going to finish my college without you
@shomusbiologyofficial
3 жыл бұрын
Glad to hear that you're getting benefit from my lectures. Please subscribe and share
@marsianzzz2442
10 күн бұрын
Hello sir, i have been wathing your videos since my 11th ...12th and now I'm in my 3rd yr biotech. Your efforts are truly ...amazing and hatss off to you for the efforts u put to make such good quality explaination over quantity. ✨💥😁
@shomusbiologyofficial
10 күн бұрын
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
@mallikabhattarai8414
5 жыл бұрын
You are my favorite teacher, sir . thank you so much Sir.. I just love your way of teaching..
@aritribhattacharyya
4 жыл бұрын
I have a few questions as stated below: 1) why did we attach the negative electrode to the SDS-gel if it already contains a negative component i.e. the sulphate that helped to denature the protein? 2) If multiple Ab binds to a specific protein,is there a chance to get improper result? 3) When did we add the Luminol?
@kayceedey4u679
2 жыл бұрын
A key part of the lecture he missed is the role of SDS and that West blotting detects Amino acid (AA) sequence for proteins (other blots detect nucleic acid sequence). AA have a Verity of charges. On treating the denatured protein (I.e AAs) with SDS, the SDS coats the surface of the unfolded AAs to give the protein a net negative charge. Hence the proteins have a -ve charge and move towards the positive electrode attached to the membrane. You need both -ve and +be fields to run electrophoresis. 2. Antibodies are usually highly specific to antigens, but in this case,some stray away and bind to the membrane (Hence "blocking" is necessary or you get false +ve results) 3. The secondary antibody which binds to the primary antibody (an anti-immunoglobulin) is conjugated with an enzyme eg Horse radish peroxidase (HRP). HRP catalyzes the degradation of the substrate luminol. As Luminol degrades, it emits light the position of which is detectable on x-ray film
@talhamunir6554
4 жыл бұрын
Why can't we use directly some specific enzymes that bind to Fc of primary antibody and give us result without the expenditure of 2° antibody ?
@GeneticsLessons
2 жыл бұрын
The secondary antibody binds to the primary antibody but not any target that is present in the specimen. Secondary antibodies bind to the heavy chains of primary antibodies so that they don’t interfere with the primary antibody binding to the target. This secondary antibody is made in a species that is different than both those of the primary antibody or the specimen. This minimizes non-specific binding that leads to false positives and high background noise.
@mirudulae9396
5 жыл бұрын
Can't we attach some fluorescence molecule directly to primary antibody and visualize or can't we add the enzyme to the primary antibody itself instead of secondary antibody?
@GeneticsLessons
2 жыл бұрын
The secondary antibody binds to the primary antibody but not any target that is present in the specimen. Secondary antibodies bind to the heavy chains of primary antibodies so that they don’t interfere with the primary antibody binding to the target. This secondary antibody is made in a species that is different than both those of the primary antibody or the specimen. This minimizes non-specific binding that leads to false positives and high background noise.
@rajlaxmiprasad7087
4 жыл бұрын
Respect for U.
@jyoti2298
4 жыл бұрын
You are the best teacher ever 😊😊😊😊 all the very best sir ❤️.
@shomusbiologyofficial
4 жыл бұрын
Glad to hear that you're getting benefit from my lectures
@arzoomalhotra9896
2 жыл бұрын
thanks for video , i have exam today , if this comes in exam i will bless you by heart still thank you
@shomusbiologyofficial
2 жыл бұрын
All the best
@sampuranchakraborty9606
4 жыл бұрын
Sir, I have a doubt... In Western blotting, we are using a secondary antibody attached with an enzyme that in the end provides detection... Why can't we use the primary antibody directly for that purpose attaching the enzyme there only... Won't that reduce the cost of developing another antibody... Or is that a necessary step... Please explain sir...
@shomusbiologyofficial
4 жыл бұрын
Using only primary antibody can cause more nonspecific binding hence more background noise
@amavivian1008
4 жыл бұрын
God bless you Sir, I have an exam in two weeks, and this information was really helpful🙏🙏
@shomusbiologyofficial
4 жыл бұрын
Glad to hear that you're getting benefit from my lectures
@farhat8257
10 ай бұрын
Thank you so much sir 🙏. Allah bless you with health and wealth and happiness
@shomusbiologyofficial
10 ай бұрын
Thank you so much for appreciating my efforts
@user-wk2gp3tu8h
3 жыл бұрын
Thank you sir ❤️❤️👍🏻
@shomusbiologyofficial
3 жыл бұрын
You're welcome
@abh3835
Жыл бұрын
Informative.. Good explanation.. Thank you
@shomusbiologyofficial
Жыл бұрын
You're welcome
@akankshakadukar2834
2 жыл бұрын
Thank uh so much sir.... Lots of love and support from Maharashtra 🙏❤
@shomusbiologyofficial
2 жыл бұрын
Thank you so much for appreciating my efforts
@blossommingupdaybyday6075
4 жыл бұрын
I am saying now u will become definitely a great person tomorrow. This society should have a good teacher like u☺️
@shomusbiologyofficial
4 жыл бұрын
Thank you so much for appreciating my efforts
@rithuvarna1977
4 жыл бұрын
Sir thankyou so much...well explained...
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
@kalyanpadyala1836
3 жыл бұрын
sir ,why cant we add enzyme to the primary antibody itself so our test would be simplified?
@GeneticsLessons
2 жыл бұрын
The secondary antibody binds to the primary antibody but not any target that is present in the specimen. Secondary antibodies bind to the heavy chains of primary antibodies so that they don’t interfere with the primary antibody binding to the target. This secondary antibody is made in a species that is different than both those of the primary antibody or the specimen. This minimizes non-specific binding that leads to false positives and high background noise.
@kangkanamedhi4060
4 жыл бұрын
After watching it i m excited to perform it in lab. Thank u sir u r the best teacher
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
@raveendissanayaka6778
2 жыл бұрын
Super💪💪💪💪👍👍❤❤💐💐 Thank you very much
@shomusbiologyofficial
2 жыл бұрын
You're welcome
@VishalGupta-iq2ev
2 жыл бұрын
really to good for the beginner as well as experienced graduate and postgraduate
@shomusbiologyofficial
2 жыл бұрын
Thank you
@surabhisharma3514
2 жыл бұрын
Thank you so much sir! It helped alot. I just have one doubt is the antibody not going to bind with the dry milk since milk also have protein in it.
@shomusbiologyofficial
2 жыл бұрын
Yes.
@surabhisharma3514
2 жыл бұрын
@@shomusbiologyofficial okay Sir. Thank you so much!
@aruncp4263
5 жыл бұрын
aftr an hour I have sem exm.. Tq 4 ur class .. full support
@shomusbiologyofficial
5 жыл бұрын
You're welcome
@alhudaonlinequranacadmey6158
2 жыл бұрын
Our teacher always coping you to teach us in class😂😂 same to same
@shomusbiologyofficial
2 жыл бұрын
Good to know
@pandeydivyjyoti
5 жыл бұрын
Explained far better than my professor. Thank you 👍
@shomusbiologyofficial
5 жыл бұрын
Thank you. Glad you liked my lectures
@Wiselysane
Жыл бұрын
Sir thankyou VERY VERY MUCH 💖
@shomusbiologyofficial
Жыл бұрын
You're welcome
@swetayadav28_13
Жыл бұрын
This channel is a saviour when there is no tym..🔥💯
@shomusbiologyofficial
Жыл бұрын
You're welcome
@johntindell9591
3 жыл бұрын
Thank you so much.
@shomusbiologyofficial
3 жыл бұрын
You're welcome
@kingshukkalita676
4 жыл бұрын
You are legend, Sir. what an explanation.....superb...
@shomusbiologyofficial
4 жыл бұрын
Thank you so much for appreciating my efforts
@sreyadas9272
4 ай бұрын
Oh Sir another concept clearing video❤
@shomusbiologyofficial
4 ай бұрын
Thank you so much for appreciating my efforts
@syedhasaanabbas1697
Жыл бұрын
Ur explaining very well kept it up . Lots of love
@shomusbiologyofficial
Жыл бұрын
Thank you
@Courtandhayleyshow
Жыл бұрын
thank you for explaining how you did!
@shomusbiologyofficial
Жыл бұрын
You're welcome
@saginelasailaja9950
3 жыл бұрын
More much good explanation sir.
@shomusbiologyofficial
3 жыл бұрын
You're welcome
@setarehsohail5422
2 жыл бұрын
Hi....I have a question. If I induce the cells with a Plasmid that fuse with BioID and then they get treatment with Biotin, what I would expect to see in SDS-PAGE using Streptavidin fluor Fluorescent? Do I need to apply for primary and secondary Antibodies? Or Streptavidin flour Fluorescent is enough? I would appreciate your answer.
@ngrakesh3064
Жыл бұрын
This week we executed this and it is remarkable.
@shomusbiologyofficial
Жыл бұрын
It is remarkable in deed
@rohith9732
5 жыл бұрын
Thank u sir i had written what u said in all blotting topics. Thank you very much for for the knowledge u given to me
@shomusbiologyofficial
5 жыл бұрын
Thank you. Glad you liked my lectures
@TeJa_BFSc
5 жыл бұрын
Super raa...😂😂
@noorsbiology7580
3 жыл бұрын
LEGEND
@shomusbiologyofficial
3 жыл бұрын
Thank you
@Naveenkumar-wt6qp
Жыл бұрын
excellent, being a chemist, still I could understand very nicely. Thanks for your clear explanation
@shomusbiologyofficial
Жыл бұрын
Thank you
@marambawazir6413
4 жыл бұрын
Your work is highly understandable I appreciate it thanks
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
@nameerahwajahat7494
4 жыл бұрын
you are the shahrukh khan of biolgy thank u shomu
@shomusbiologyofficial
4 жыл бұрын
You're welcome
@anagantisandhya644
3 жыл бұрын
Thank u sir for urs traching while comparing my lecturer u teaching is super
@shomusbiologyofficial
3 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
@anagantisandhya644
3 жыл бұрын
K sir
@rafiaaziz9441
4 жыл бұрын
👌
@harshithams7823
Жыл бұрын
Thank you soooooooo much sir🙏🏼🙏🏼🙏🏼
@shomusbiologyofficial
Жыл бұрын
You're welcome
@revocatusredemtus9701
3 жыл бұрын
respect to you the biotechnologist genious
@shomusbiologyofficial
3 жыл бұрын
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
@punamchopade8387
4 жыл бұрын
This information is very useful for me but sry to say that Vedio quality is not good.
@gravity62
Жыл бұрын
Your explanation was great.
@shomusbiologyofficial
Жыл бұрын
Thank you
@layalharb2472
4 жыл бұрын
Thanks a lot😍😍😍😍😍😍 very helpful
@shomusbiologyofficial
4 жыл бұрын
You're welcome
@ahmedmosadalameldin3135
8 жыл бұрын
Great informative explanation, greets from Egypt bro.
@gilgamesh7679
Жыл бұрын
Sir how do the primary Ab bind with protein since we changed the protein structure to primary during sds page?
@akashmishra1285
4 жыл бұрын
What if during blocking process the detergent blocks the target protein also and how it is prevented??
@ackahmichael449
3 жыл бұрын
Thank you so much for this wonderful work. My question is " how do we know that the primary antibody will only bind to our target protein"?
@riffatniazi363
3 жыл бұрын
As he mentioned that we will use blocking buffers like BSA (bovine serum albumin) that will do what...that will block yhe rest of the space so that the primary antibody could only bind to that specific site not at the unnecessary site!
@swetapaul3758
3 жыл бұрын
Primary antibody will only bind to our target protein because the antibody is designed so just a way that it can only bind to our target protein if that particular protein is present in the mixture.
@rajimubarak6108
2 жыл бұрын
Shomu you too Sabi book. You explain everything so succinctly. Well done.
@shomusbiologyofficial
2 жыл бұрын
Thank you so much for appreciating my efforts
@tapashpaul1156
3 жыл бұрын
You are Awesome sir 👍👍👍👌👌👌
@shomusbiologyofficial
3 жыл бұрын
Thank you
@sachinsangle8916
3 жыл бұрын
Tqqq sm bro ... doing really great work. ...👍👍 God bless you..❤️
@shomusbiologyofficial
3 жыл бұрын
You're welcome
@Shadrackndaro
3 ай бұрын
We muhindi yan dakika kumi na saba zote
@risharajkhowa5060
Жыл бұрын
Sir what a explanation !😍
@shomusbiologyofficial
Жыл бұрын
Thank you so much for appreciating my efforts
@parthankam5968
3 жыл бұрын
10:23 That stare is hella priceless
@harshadaaaa26
3 жыл бұрын
I felt the same.. That pause felt so imp for some reason
@khushijindal2753
Жыл бұрын
If your videos didnt exist i wouldnt even pass my bitechnology paper ,thank you so much sir your skills have got me inspired.Great Respect to You !!!
@shomusbiologyofficial
Жыл бұрын
You're welcome
@iamajax
5 жыл бұрын
Thanks alot sir , my exam is on 14th of May and u helped me alot in this topic ...anyways you help me everytime..hehe ..you are a genius sir..may god bless you ...❤️
@shomusbiologyofficial
5 жыл бұрын
Thank you. Glad you liked my lectures
@karinaburgess3989
5 ай бұрын
Seriously best teacher for this on the internet THANK YOU
@shomusbiologyofficial
5 ай бұрын
Thank you so much for appreciating my efforts
@tahseenhashmi5885
2 жыл бұрын
You are an encyclopedia.
@shomusbiologyofficial
2 жыл бұрын
Thank you so much for the compliment. Stay tuned for more
@ااااا-ن9ي8م
10 ай бұрын
I love the way you teach ❤
@shomusbiologyofficial
10 ай бұрын
Thank you
@pranjalimahamuni-badiger2873
Жыл бұрын
No one can explain with such a beutiful way u explain. Thanku very much
@shomusbiologyofficial
Жыл бұрын
You're welcome
@thisumamarasinghe8171
7 ай бұрын
big round of aplause for that comprehensive explanation
@shomusbiologyofficial
7 ай бұрын
Thank you
@farahdeeba6039
2 жыл бұрын
Sir your teaching style is awesome...l understand everything which you used to deliver.
@shomusbiologyofficial
2 жыл бұрын
Thank you so much for appreciating my efforts
@Makeyourlifeeasy78
2 жыл бұрын
you are really good deliver the lectures Alhumdullilah my all blotting are cleared now ...Thank u soo much
@shomusbiologyofficial
2 жыл бұрын
Thank you so much for appreciating my efforts. Stay tuned
@payalpatel9152
3 жыл бұрын
Video was helpful
@shomusbiologyofficial
3 жыл бұрын
Thank you
@payalpatel9152
3 жыл бұрын
Video was helpful
@shomusbiologyofficial
3 жыл бұрын
You're welcome
@meghna406
2 жыл бұрын
Thank you so much ❤️
@shomusbiologyofficial
2 жыл бұрын
You're welcome
@teeemmgee8792
4 жыл бұрын
Instead of using sec. Antibody... U can attach any fluroscence molecule to the primary antibody... And can check the fluroroscence..??
@GeneticsLessons
2 жыл бұрын
The secondary antibody binds to the primary antibody but not any target that is present in the specimen. Secondary antibodies bind to the heavy chains of primary antibodies so that they don’t interfere with the primary antibody binding to the target. This secondary antibody is made in a species that is different than both those of the primary antibody or the specimen. This minimizes non-specific binding that leads to false positives and high background noise.
@alina.m4lina
2 жыл бұрын
Shomu, our biology professor asked us to take notes on this video for homework! you rock!!:))
@shomusbiologyofficial
2 жыл бұрын
Thank you so much for appreciating my efforts
@Maniraam2662
3 жыл бұрын
Recently i got tested with western blot for hiv... But i got all bands were negative but control band was positive (present ) what does it mean sir...
@GeneticsLessons
2 жыл бұрын
That you are clean. Control band is needed to prove that test is working.
@briantracy271
8 жыл бұрын
shomus you are great bro ..are you a student or teacher?
@shomusbiologyofficial
8 жыл бұрын
+lumberjack s I am a learner
@anxiousrat9658
Жыл бұрын
i have an exam tommorow and this really helped thank you 🙏🏼
@shomusbiologyofficial
Жыл бұрын
You're welcome
@nammakusooruthanmukkiyam6939
4 жыл бұрын
Video sound very low ..pls do something
@jyoti2298
4 жыл бұрын
Thank you sooo much sir for this good explanation ❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️
@shomusbiologyofficial
4 жыл бұрын
Glad to hear that you're getting benefit from my lectures
@florasays1730
4 жыл бұрын
thankewww so muchhh
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
@esraaabd-allah1626
6 жыл бұрын
That was a very simple and perfect explanation..Thank you very much ☺
@Pen_and_Pencil
Жыл бұрын
Thank you Sir!
@shomusbiologyofficial
Жыл бұрын
You're welcome
@tamanaah-u4g
9 ай бұрын
Thank you sir
@shomusbiologyofficial
9 ай бұрын
You're welcome
@samgaming91632
3 жыл бұрын
you're studying things in really easy ways, I always recommend your videos to my friends
@simrankaur2471
3 жыл бұрын
Thankyou sir💓
@shomusbiologyofficial
3 жыл бұрын
You're welcome
@anshulrana5614
Жыл бұрын
Good morning sir. Sir I want to know that before performing westerns bloating we labelled DNA 🧬 actually sir I do western bloating at my training period this time but ma’am do biotin 3 end DNA labelling 🤥🤥 I didn’t get it why
Пікірлер: 527