Your lectures are always worthy Dr. Banta. Thank you. For an organism with 2 pairs of chromosomes, during meiosis there could be possibility of 2 to power 2 of varients. Therefore we, for humans there are 2 to power 23 possibilities. Thus we could imagine how many varients/diversity could be generated in just one generation. This is essential in haplotyes because they are inheritance togather to next generation and very often useful in population genetics.
@dr.farazakrim2239
Жыл бұрын
You are doing excellent job sir 👍
@heba6746
Жыл бұрын
Very informative thank you
@myeong-hyeonmin5229
Жыл бұрын
Thank you all for your valuable contribution
@pradnyachoukekar9203
11 ай бұрын
Thank you!!!!!
@emilymoreau5045
9 ай бұрын
love this! he's great!
@irfanmauludin398
9 ай бұрын
so how do you know that someone that have long combination of Y cromosome DNA more ancient than shorter combination, did you already prove it with oldest corpse just like Pharaoh Ramses II or king of Babilonia or king of Akkadian or corpse of Sumerian People?
@monkaydgoofy2059
Жыл бұрын
I'm new to all this and your vid helped me understand quite a few things. When I was designing Primers and checking the amplicon in the UCSC Genome Browser, I came across an alternate sequence labelled " chrX_MU273397v1_alt:236887-237284 " . Clicking on it said " Alternate Haplotype Sequence " Could you explain haplotype block in this context coz the PCR product formed has non specific amplifications and no matter which primer I take has the same alternate amplicon. How should I troubleshoot if it is caused due to Haplotypes?
@DrJBanta
Жыл бұрын
Hello, it sounds like you are using nuclear DNA instead of haplotypic mitochondrial or Y-chromosome DNA. Do I have that right? Best, Josh
@monkaydgoofy2059
Жыл бұрын
@@DrJBanta Yh I'm using nuclear DNA. what should I do, i got no idea
@DrJBanta
Жыл бұрын
@@monkaydgoofy2059 Ok, that helps to figure this out. The problem is that you can only create a haplotype network for haplotypic data, such as mitochondrial or X-chromosome or Y-chromosome data, or bacterial DNA. For your data, you will want to perform a phylogenetic analysis. See my tutorials on my website (joshbanta.com) Best, Josh
@monkaydgoofy2059
Жыл бұрын
@@DrJBanta Thanks for the reply. I wanted to ask u something else. For my project I sequenced the Dna of 5 patients and tested for mutations in 3 genes. No exonic or splice mutations found. However all 5 of them had a deep intronic variant of a single base substitution in the same exon in the same gene. Can u tell if it's significant or where can I learn more about similar cases?
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