References/Resources: / the_crux
This video discusses the general biology of yeast cloning vectors. There are 5 classic vectors - Integrating, Episomal, Replicating, Centromeric, and Artificial Chromosomes - as well as the naturally occurring Yeast 2 mu plasmid. Yeast vectors rely on coli-based vectors like pBR322, pUC19, or Bluescripts. Typically, yeast cloning vectors tend to be suboptimal due to the complexity of the organism (vs. bacteria). This video highlights how these vectors can be used, their advantages, and disadvantages.
Jump to your favorite section:
00:00 Cloning in Yeast
01:11 Shuttle vectors
01:54 Auxotrophy
03:33 Summary of Yeast vectors
04:53 YIps
08:28 YEps
10:43 YRps
13:48 YCps
15:25 YACs (Linear)
18:34 YACs are terrible!
20:02 YACs (Circular)
Patreon Page: / the_crux
Suggested videos:
Plasmids: • Plasmid and Phagemid c...
Yeast (ARS): • DNA Replication - Orig...
BACs: • F plasmid, BACs, Fosmi...
Must watch playlists
All videos on Genetic Engineering: • Genetic Engineering (G...
All videos on DNA Replication: • All about DNA Replicat...
All videos on Transcription: • All about Transcription!
All videos on Translation: • All about Translation!
Consider supporting the growth of this channel by becoming a Patron: / the_crux
I appreciate your support :)
Негізгі бет Yeast cloning vectors - YIp, YEp, YRp, YCp, YACs - Shuttle plasmid vectors
Пікірлер: 10