Thank you so much for biref and comperehensive detailed video now the concept it clear, highly appreciated
@asifmolbio
4 ай бұрын
Glad it was helpful!
@sabamudassir1846
6 ай бұрын
Thank you soo much sir jazakallah my concept is much clear after watching your videos
@asifmolbio
6 ай бұрын
Glad if its helpful
@shafiqturi3818
8 ай бұрын
Genome r transcriptome ki aj samjh agai Dr. Urdu b sath bola krein tu best huga.🌹
@asifmolbio
8 ай бұрын
But my audience is also from English countries. Urdu will make me limited to india and Pakistan
@zeeshanshokat5178
9 ай бұрын
Thank you, much appreciated
@asifmolbio
9 ай бұрын
Thanks for appreciating
@muhammadshoaib-c2w
9 ай бұрын
Thanks, brother .its really helpful.
@asifmolbio
9 ай бұрын
Glad if its helping
@belief6695
8 ай бұрын
Sir.. how to processs array data.. where gene name is not given... .. how can we find gene name... plz let me know..
@asifmolbio
8 ай бұрын
There should be transcript IDs
@mubasshirhussain
9 ай бұрын
Hello sir, your videos are very helpful. Can you please guide us how to quantify root colonization of fungi by qPCR? What will be the pcr experiment design and how many controls we can use?
@asifmolbio
9 ай бұрын
Quantifying root colonization of fungi by quantitative polymerase chain reaction (qPCR) involves extracting DNA from root samples and amplifying fungal DNA using specific primers. Standardize your protocol, optimize primer efficiency, and use a reference gene for normalization. Measure the cycle threshold (Ct) values and analyze data with appropriate software. Remember to include appropriate controls and validate your qPCR assay.
@mubasshirhussain
9 ай бұрын
@@asifmolbio Thank you so much 🫡🫶
@asifmolbio
9 ай бұрын
Glad if it’s helpful
@snehaunni194
5 ай бұрын
Is it possible to take different leaves from a same plant for diiferent interbvel to study abiotic stress.
@asifmolbio
5 ай бұрын
For transcriptomic its not recommended
@snehaunni194
5 ай бұрын
@@asifmolbio what about real time pcr analysis.. Is it possible to collect leaves from the same plant
@asifmolbio
5 ай бұрын
No, for RT-PCR and transcriptome analyses instructions are same
@muhammadshoaib-c2w
9 ай бұрын
if we cut the leaves, and we also want to take root samples after that. does it also affect root RNA by cutting injury of leaves? so what is the solution?
@asifmolbio
9 ай бұрын
First take leaves from intact plants, save these samples. Then quickly root out plant clean the roots and save then as well
@mushtaqnajar8930
9 ай бұрын
Brother, app ko shyad naslaa hogyaa hai..khyaal rakhyy apna bhai
@asifmolbio
9 ай бұрын
Haha yes you are right, i got heavy sneezing before i entered studio. Thanks for concerns
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