This clears up so much, I was so confused trying to learn this on my own
@josecordova32
Жыл бұрын
This is one of my core competency requirements of my program, this helps so much!
@patricktracy9947
Жыл бұрын
I am glad it was helpful.
@jamesboulger8705
3 жыл бұрын
Thank you so much for the video, some videos for this are like an hour long, and you are summing it up so nicely. It is a nice review because I basically know what I am doing, but need to make it all crystal clear for a practical coming up.
@patricktracy9947
3 жыл бұрын
Hi James, yes I paid attention to the length of the videos while making them. Keep is concise. I am glad the video was helpful.
@seriesmovie4871
3 жыл бұрын
I didn't know this could be this easy! Thank you very much!
@patricktracy9947
3 жыл бұрын
You're welcome.
@ruvimbomujawo5853
5 жыл бұрын
Absolutely amazing and easy to understand!! Thank you for this video
@ericyother1512
2 жыл бұрын
This helped a ton. I understood the steps prior to watching this but I understand the logic now. Thanks!
@patricktracy9947
2 жыл бұрын
Thanks Eric...I am glad it was helpful.
@Theshamruck
2 жыл бұрын
As an MLT student, thanks for the explanation!
@maicoabella6628
5 жыл бұрын
Super thank you somewhat it gives me an idea i been trying to understand this one for quite a long time now..
@patricktracy9947
5 жыл бұрын
You are welcome.
@charissemarietoledo2820
4 жыл бұрын
Thanks for this video...im preparing for licensing exams in clin lab scientist and this video is a big help😉
@patricktracy9947
4 жыл бұрын
I'm glad it was helpful.
@clairevernyuy5827
8 ай бұрын
This has been confusing for me but I understand now. Thank you!
@patricktracy9947
8 ай бұрын
Well that's good!
@sandeshjonchey1336
5 жыл бұрын
I won't be scratching my head anymore. Thank you!!!
@patricktracy9947
5 жыл бұрын
You're welcome.
@saraaljumaah6394
5 жыл бұрын
Great video, very informative Thank you
@zohrehkhavari3605
2 жыл бұрын
So clear explanation and easy to follow.Thank you
@patricktracy9947
2 жыл бұрын
I am glad it was helpful Zohreh.
@lucasau1124
3 жыл бұрын
Thank you. Easy to understand
@cristinavall8587
6 жыл бұрын
That was so helpful thank you!!
@patricktracy9947
6 жыл бұрын
Good, that's the point!
@raveenarani2948
3 жыл бұрын
Thank you so much! this was so so helpful :) ive always tried to figure this out now 4 years later i get it!!
@patricktracy9947
3 жыл бұрын
Better late than never. :)
@williampalkow5108
5 жыл бұрын
Very informal. Thank you.
@katendemuhammed4429
4 жыл бұрын
Very helpful. A big THANK YOU for the video.
@patricktracy9947
4 жыл бұрын
You're welcome.
@petronilanzioka667
2 жыл бұрын
this made things easy for me ......thank you
@patricktracy9947
2 жыл бұрын
Hi Petronila...I am glad it was helpful. 😁
@TheGrizzly43
5 жыл бұрын
This was huge help. Thanks!
@patricktracy9947
5 жыл бұрын
You're welcome.
@nurulaimiabdulrahman7271
4 жыл бұрын
Helpful video. Thank you so much!
@patricktracy9947
4 жыл бұрын
You're welcome.
@khadijasakiko4242
4 жыл бұрын
Thank you soooooo much!!! You saved me indeed
@patricktracy9947
4 жыл бұрын
Wow! I am glad I could help.
@amrouelsayed615
6 жыл бұрын
Really thank you great effort and great job ...appreciate!!!!!
@patricktracy9947
6 жыл бұрын
You're welcome Amrou.
@sillajames1570
Жыл бұрын
Thankyou , its really informative
@patricktracy9947
Жыл бұрын
You are most welcomed.
@manarsmaan2624
2 жыл бұрын
Thank you so much Doctor for the great explanation. Can you do more vedios on discrepancy
@patricktracy9947
2 жыл бұрын
Hopefully, but not anytime soon.
@amenacristovao8566
5 жыл бұрын
Thank you for your video. it is very helpful!
@patricktracy9947
5 жыл бұрын
My pleasure
@rhulaboratory5832
3 жыл бұрын
thank you so much
@princesschanahsp1059
3 жыл бұрын
Thank you for this. In 3 years of my work, we never do antibody panel testing. I’m a bit confused - why was Jsa not crossed half? Thank you again!
@ecknareal
3 жыл бұрын
I am also wondering this, i presume it's a mistake by the person performing the screen.
@patricktracy9947
3 жыл бұрын
I gave Jsa an X and completely ruled it out because it is a low-incidence antigen. There are different ways to approach ruling out antibodies with some being more conservative than others. If I were to be very conservative, I would not have completely ruled out Jsa, but would have done further testing to completely rule it out. If this patient were to receive a crossmatch which turned out to be incompatible, then I would return to the antibody identification panel to see if a low-incidence antigen like Jsa were the issue.
@garyb5439
2 жыл бұрын
Thank you this helps a lot!
@patricktracy9947
2 жыл бұрын
I am glad it was helpful.
@fernandabolivar3873
4 жыл бұрын
Thank you so much! Great job
@patricktracy9947
4 жыл бұрын
I'm glad it was helpful.
@ashleyt1713
6 жыл бұрын
Thank you so much for your video. I have got everything)).
@patricktracy9947
6 жыл бұрын
Coolio...thanks
@Pathology92
3 жыл бұрын
Sir, we request you to kindly make videos on adsorption and elution procedures! ❤️❤️
@patricktracy9947
3 жыл бұрын
Hi Azadar, I do not teach those techniques in my program, so I am not familiar with doing them.
@Pathology92
3 жыл бұрын
@@patricktracy9947 BTW thank you sir for all these wonderful videos ❤️
@Pathology92
3 жыл бұрын
Sir which book we should use for microbiological techniques and for blood bank? Kindly, guide us.
@opiumden34
Жыл бұрын
Why is big M for donor/row 3 heterozygous? There's only M, not big M little m. Shouldn't it be homozygous? Ugh I am so confused.
@patricktracy9947
Жыл бұрын
Hi opiumden, the most common pairs that are used for determining dosage are Ee, Cc, Kk, JkaJkab, FyaFyb, MN and Ss although these are not the only pairs you need to pay attention to. As you can see, there is no m. M is paired with N. That is why M is heterozygous.
@fritzbalajadia6605
Жыл бұрын
Thank you so much for this video. I know how to cross out but after finding the antibody I don't know what to do. I don't know if I was doing the rule of 3 right.
@patricktracy9947
Жыл бұрын
Hi Fritz, applying the rule of three can be a bit tricky, especially for confirming the three positives. I could Zoom with you and explain it if that would be helpful.
@zucccccc7467
3 жыл бұрын
I've read from a textbook that K antigen is also a low prevalence antigen which may be excluded if not homozygous (an exception). I'm wondering if this exception should be followed.
@patricktracy9947
3 жыл бұрын
Hello zucccccc, every lab will write its own standard operation procedure (SOP) for testing which should address such matters. I cannot say whether K should or should not be excluded if not homozygous; it's really up to the SOP. If you are in an MLT or MLS program, ask your instructor about ways to handle ruling out low-incidence antigens.
@zucccccc7467
3 жыл бұрын
@@patricktracy9947 Thanks for the response! I meant low incidence by the way, not prevalence. Also, the textbook is by Denise Harmening.
@patricktracy9947
3 жыл бұрын
@@zucccccc7467 One thing to note, even though the K antigen doesn't appear often in a typical antibody identification antigram, I wouldn't really consider it as low-incidence. It is usually present in at least two cells, whereas Lutheran-a usually doesn't appear at all.
@gerrygadia7646
5 жыл бұрын
Thank you so much! 😁👌
@patricktracy9947
5 жыл бұрын
I hope it was helpful.
@user-hr5xu7ud3p
2 жыл бұрын
Thank you 🙏🏾
@luhhsya3798
6 жыл бұрын
thanks for this video highly appreciated, by the way, im a volunteer trainee at a component processing unit, I wonder if panocell is used for tube method, because I saw my staff also doing an antigram of Panel C, using gel card method, p.s. they're using OrthoInnova/ OrthoMax Vision machine, if ever in the future also make a video in terms of gel card and automation. thanks again! looking forward for more educational and informative tutorials, you inspire us a lot. God Bless.😇
@patricktracy9947
6 жыл бұрын
I don't teach anything in our student lab except the tube method...sorry.
@Dabz2084
4 жыл бұрын
this is how I do my cross out...it's quite different how blood bank guy does it...his double dosage is quite questionable.
@نور-ق3ل5ث
2 жыл бұрын
Thank you so much 🦋🦋..
@patricktracy9947
2 жыл бұрын
You're welcome.
@alexismendoza7331
3 жыл бұрын
Hello! I have a question. During the antibody screening, will you consider significant antibodies that showed little dosage? Loved your video btw, I always use it as reference
@OmegaPsiPhi0
6 жыл бұрын
I was shown this years ago on a path placement brill vid xxx
@patricktracy9947
6 жыл бұрын
Thanks Dan
@nunaahmad98
8 ай бұрын
mind asking, at panel 11, the JSa was (+ve) and JSb (+ve) so both was heterozygous isnt it? but why does the JSa were not single cross (/) at panel TC?
@nmz882
2 ай бұрын
I was wondering the same thing
@All_hail_zolie
17 күн бұрын
I was also wondering 'bout it too.
@playmasterangie
3 жыл бұрын
17:05 rule of three
@hdgfgfbghgghguftbahv
6 жыл бұрын
Why didn't you half-crossed the Js^a? It gave a positive reaction on the 11th cell which is a heterozygous
@patricktracy9947
6 жыл бұрын
Hi Meriam,You are right; I should have put a single slash through Js^a. This will not affect the outcome of the panel. This also illustrates how easy it is to make an oversight on all these little boxes.
@hdgfgfbghgghguftbahv
6 жыл бұрын
Patrick Tracy even though you didnt put one slash on that antibody, it was quite understandable that it was not ruled out. Thanks by the way! Great lecture 😃
@patricktracy9947
6 жыл бұрын
Thanks, I am glad it was helpful.
@manarsmaan2624
2 жыл бұрын
Can you please do a vedio on discrepancy. Thank you so much
@patricktracy9947
2 жыл бұрын
Maybe in the future.
@aglabradorable
3 жыл бұрын
thank u for this video
@patricktracy9947
3 жыл бұрын
Hi Astrud...I am glad that the video was helpful.
@mjojo7070
Жыл бұрын
wow I love` it
@paolocaselli2659
3 жыл бұрын
I liked your video a lot. Am I wrong that Lea and Leb are not alleles? Wouldn't it better to use a different strategy to rule them out?
@patricktracy9947
3 жыл бұрын
Hi Paolo, do you mean that Lea and Leb do not exhibit dosage?
@cjo7277
6 жыл бұрын
You mentioned some antigens are low incidence. Which ones are the low incidence?
@patricktracy9947
6 жыл бұрын
Look at the antigens that have all 0's or just one or two +'s.
@biosphere7762
Жыл бұрын
I look for easy procedure for cross matching
@onlymaxtheman116
4 жыл бұрын
is there sufficient evidence to prove the suspected antibody or Is the patient lacking the antigen corresponding to the antibody?
@patricktracy9947
4 жыл бұрын
First of all, in order for a person to produce an antibody targeted at RBCs, they MUST lack the corresponding antigen. Second, the antibody identification method used in this video is routine and would be reliable in most situations. There was nothing out of the ordinary with this patient, so yes I am confident that there is sufficient evidence.
@onlymaxtheman116
4 жыл бұрын
In addition to the rule of three, do you confirm your antibody using antigen typing or not ? and what source do you use to confirm your antibody?
@patricktracy9947
4 жыл бұрын
With this patient, we did not use antigen typing to confirm the antibody. Under normal conditions, we wouldn't do that. We mainly do antibody screening and identification to support crossmatches. If for some reason an antibody went undetected in the screen or was misidentified, it should be detected in the crossmatch if the facility has a crossmatch procedure which uses the indirect antibody test with anti-human globulin.
@onlymaxtheman116
4 жыл бұрын
Well thank you so much- your information helps me to improve my research. All the best
@annaly6612
4 жыл бұрын
Why isn’t Jk crossed out in technical cells (Kell column)? It was positive and by itself...
@patricktracy9947
4 жыл бұрын
I don't respond to questions that are inaccurate. There is no Jk in the Kell antigens listed on the antigram.
@annaly6612
4 жыл бұрын
Patrick Tracy It was a typo. I meant Js under Kell antigens in technical cells. It was positive and by itself so wondering why wasn’t it crossed out?
@patricktracy1057
4 жыл бұрын
@@annaly6612 Again I have incomplete information. You refer to Js. Is that Jsa or Jsb? The reason I am being particular about this is because it seems like you are not closely paying attention, and that is what causes mistakes.
@kk174
3 ай бұрын
You haven't single crossed "Jsa" yet it is homozygous in TC. Do we not take TC into consideration. Please help I am dumb 🗿
@patricktracy9947
3 ай бұрын
Hi kk...your observation is correct, and I address it in the notes section.
@nunaahmad98
10 ай бұрын
i dont understand, why does Big M was heterozygous and it should be given single cross at first? more confusing, it gets homozygous and double cross then. do you mind explaining it?
@julevibernido
10 ай бұрын
Hi, M is heterozygous because MN is a pair not Mm. This is a unique group of antibodies-MN Ss. You can also read the previous comments for reference where the lecturer points that out.
@patricktracy9947
10 ай бұрын
Thanks Jule :)
@gousgamingyt220
2 жыл бұрын
Hi sir my doctor told me to identify the agenest antibody wt can I do it's curable to medicine
@patricktracy9947
2 жыл бұрын
Hi Gous, I do not understand your question.
@Apratim98
3 жыл бұрын
Is alloantibody screening must or optional before transfusing PRBCs? And after TYPING what's the rationale behind doing antibody screening with blood type O first rather than directly going for cross matching between patient serum and sample from actual PRBC unit we're gonna use?
@patricktracy9947
3 жыл бұрын
Theoretically, the antibody screen is not needed, but I don't know of any blood banks that would not do it. The screen may detect antibodies that may not be detected in the crossmatch.
@Pathology92
3 жыл бұрын
I think C.M is the final check for ABO compatibility. Now if the patient is having weakly reactive alloantibody( Let say it anti-jka) and donor is heterozygous for that antigen[ jk(a+b+)] then in cross match the alloantibody may not be detected( because few antigens are available for weakly reactive Ab) but on screen it should react because screen cells are homozygous[ jk(a+b-) ]for the antigen( all the antigens are jka on screen cells) We use 'O' cells in Ab screen because they don't have A or B antigens and don't react with anti-A or anti-B antibodies of patient and only detects non ABO antibodies. Sorry for the bad English 😂
@saudiseeker6061
2 жыл бұрын
Do you need to use donor cells to do Ab identification for patient? Or just the company reagents (11panel)!? Because on the left i see (Donor) written over there
@patricktracy9947
2 жыл бұрын
The antibody screen and panel reagent cells are labeled as "donor" because the RBCs in the reagent come from human donors. Do not confuse them with a donor of a unit of RBCs.
@saudiseeker6061
2 жыл бұрын
@@patricktracy9947 yes now i understand, thanks ❤️🌹🌹
@guramayleethiopia5984
4 жыл бұрын
10q........it is really helpful
@patricktracy9947
4 жыл бұрын
Thank you...I am glad it was helpful.
@shelliedenebennett3268
5 жыл бұрын
Kelly doesn't show dosage, neither does Lutheran.......or lewis........yet u cross out in a way tht suggests tht they do.....eg: u could have fully crossed out the Lutherans at cell 8 yet I'm seeing a single cross through Lutheran a
@patricktracy9947
5 жыл бұрын
I am just being conservative. Some blood bankers believe all antigen pairs should be treated as if they exhibit dosage. It's better for students to learn a cautious approach. When they are certified techs working in the field, they can expand their approach to blood banking while in the confines of the standard operating procedures of the lab they work in.
@mlos2424
4 жыл бұрын
Hi. Hope you're having a good day. If I may ask, what does the IS phase stand for, initial or immediate spin? Or is it the same?
@patricktracy9947
4 жыл бұрын
Hi, yes IS stands for immediate spin, which really is the same as initial spin, so they are the same.
@sahla102
6 жыл бұрын
I'm seeing this to learn for Licensure exam.. please tel how homozygous and heterozygous understands?? ..
@patricktracy9947
6 жыл бұрын
I recommend the following links.www.bbguy.org/education/glossary/gld15/www.bbguy.org/education/videos/antibodyid1/
@shelliedenebennett3268
5 жыл бұрын
Is there a reason y u cross out tht way?
@PRubin-rh4sr
2 жыл бұрын
I love you
@MyMomSayNoDota
5 жыл бұрын
it's like calculating lottery number
@patricktracy9947
5 жыл бұрын
Yes, something like that. :)
@okike.4573
5 жыл бұрын
10:03 why didnt you totally cross out the M?
@patricktracy9947
5 жыл бұрын
Because it is heterozygous with N. I only completely cross out an antigen with an X if it is homozygous.
@shibinsabu5779
6 жыл бұрын
Sir looking for blood bank job.i have 5 years of experience.can you help me?
@patricktracy9947
6 жыл бұрын
I really don't know what I can do for you.
@JamesG...
2 күн бұрын
Lutheran.
@chantelle5170
2 жыл бұрын
bruh
@nleiwk6945
3 жыл бұрын
can you help me with my transfusion assignment? if yes please send me your email
@patricktracy9947
3 жыл бұрын
ptracy@wvc.edu
@UrSOpimp
5 жыл бұрын
Hi, so i know some people put a scribble for kell to differentiate big from small. Does the scribble mean big K?
@patricktracy9947
5 жыл бұрын
Sorry, I am not sure what you mean by scribble. When writing antibodies big C and little c, we put a line over the top of little c to make sure it is not confused with big C. For big K and little k, there is a slight difference in how they are written to indicate which one is big and which one is small. I cannot show you here, because I can only type.
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