Caco2 is The human colon epithelial cancer cell line,
And the cell line is used as a model of human intestinal absorption of drugs.
This model can test a compound suitability for... oral dosing, prediction of intestinal permeability and also investigation of drug efflux.
Important characteriristics of caco2 cell lines
Growth in culture
They are cultured as monolayer epithelial cells.
Caco 2 cells form a tight monolayer growing on a permeable filter support.
Differentiation
When Caco2 cells are cultured as a monolayer, they differentiate to form tight junctions between cells, to serve as a model of passive diffusion of compounds across the monolayer.
The differentiation process is a 21 day system in standard culture medium
Morphology
The cells show polarity and posess tight junctions and brush borders.
Now the permeability of the compounds can be measured in both directions.... That is ...apical to basolateral and... Basolateral to Apical...
AB corresponds to absorption from intestinal lumen to the circulation.
Негізгі бет Caco 2 Cells Permeability Assay
Пікірлер: 8