I understood in 3:59 minutes what I've been trying to understand for hours on the internet. Thank you!
@thermofisher
6 жыл бұрын
That's amazing to hear, thank you!
@JohnOven
10 жыл бұрын
This is an *Incredible* video that shows with ease and very clear simple animations how TaqMAN works. Great Job!
@famligtenberg9081
2 жыл бұрын
This is the first time I see a video of Taqman, but I am already considering a fanclub.
@Schulemachtmuede
10 жыл бұрын
Easily understandable explanation and good-looking animation. A thank you from a German student.
@arungaur1677
5 жыл бұрын
Trafficlight Please tell Me in words how this technology is better the Dan real time pcr.
@elliet95
7 жыл бұрын
Aw, this guy is cutest! But in seriousness - very concise and informative! Thank you for the explanation :)
@stoolpigeon4285
8 жыл бұрын
Excellent summary. I've been doing this for years, and I'm not sure if I ever thought about why the probe isn't extended like the primer is, but the 'not free -OH group' explained it. Thanks
@meryamlamsissi6036
8 жыл бұрын
neeey friends ! not taq polymerase !! haha I just looove the way you explain and make the science funn ! :D
@raghi769
11 ай бұрын
I love how you explaining everything with enthusiasm!!
@hottamale180
7 жыл бұрын
very enthusiastic guy
@CromossomaX
9 жыл бұрын
This is a great video, simple, clear and very well designed!!! also love the enthusiasm of the narrator!!
@Jumboo364
8 жыл бұрын
I am so impressed by the clarity of his explanation. Thank you!
@thermofisher
8 жыл бұрын
Thanks Blair! Let us know if we can answer any questions!
@hamzerpanzer
Ай бұрын
This dude has such an infectious enthusiasm for this stuff, no pun intended
@meenabarot2037
Жыл бұрын
thank you. short and crisp explanation!
@MrsPsych
7 жыл бұрын
Such an infectious presenter. Love it!
@Sultandurrani125
2 жыл бұрын
I don't know why but listening to this man is making me happy
@dumindaamila9489
2 жыл бұрын
You are The greatest teacher i have ever seen...!!!
@daniadiya3299
11 жыл бұрын
This video made my life way easier! thanks to you TAQMAN!!!!
@pablohoney
9 жыл бұрын
This is a great video, simple, clear and very well designed. The explanations perfectly fit the images. I personally like the enthusiasm of the narrator. Thanks for the help!
@ThemisGeorgoudis
7 жыл бұрын
Thank you guys! Greetings from Greece!
@raanoooshh9296
4 жыл бұрын
Your energy was fluorescenting throughout the whole video - love that.
@thermofisher
4 жыл бұрын
Science can be Taqsing on the mind and body - a true, unadulterated enthusiasm for the work goes a long way.
@Nangyall
11 ай бұрын
The best ever lecture on Taqman probe.....Thank you❤❤
@Empress426
6 жыл бұрын
Very enthusiastic, made the video enjoyable to watch.
@1awlsusie
8 жыл бұрын
Well presented! I love this video so much!
@PatFlattery
11 жыл бұрын
YOU'RE AWESOME TAQMAN!
@madhurmotwani9867
8 жыл бұрын
Brilliant explanation. I like the enthusiasm he exudes. Thanks you.
@jannaarmstrong4048
4 жыл бұрын
Great job! Love the enthusiasm and ease of understanding this process!
@thermofisher
4 жыл бұрын
Not everything in science is as easy as we'd like it to be! We're being the edutainment we want to see in the world.
@chrisjay7750
9 жыл бұрын
Hahaha Made my revision much simpler! Thanks :D
@tamirissociareli6468
5 жыл бұрын
Great video!!
@Demolasha
10 жыл бұрын
Perfect for my MEDSCI 203 paper!! Thank you from New Zealand :)
@AnnieEngqvist
4 жыл бұрын
Great video. Your enthusiasm makes it so interesting!
@thermofisher
4 жыл бұрын
You're never too old for edutainment! That's our (unofficial) policy.
@jyotidubey4890
3 жыл бұрын
@@thermofisher 😍
@nicolassardella7897
6 жыл бұрын
Great explanation, and the enthusiasm about the topic is awesome!
@anacarolinepaivagandara2120
9 жыл бұрын
Very, very elucidative! Thank you!
@lala-kuzi
8 жыл бұрын
Thank you so much! This is the very best explanation for taq man! Short and clear! like all of your videos. You helped me so much to set up a good "methods" part in my master thesis ;) greetings from germany!
@thermofisher
8 жыл бұрын
+Lara Kuerzer Thanks, Lara! We appreciate the feedback and let us know if we can answer any questions for you. Good luck on your thesis!
@frqansafaaa6370
5 жыл бұрын
Hi lara
@bryanbenninghoff3286
11 жыл бұрын
Thank you, this is very helpful
@lifetechnologiescorp8544
11 жыл бұрын
You're very welcome, Bryan. Let us know if we can help with anything.
@miaa981
3 жыл бұрын
AMAZING! THANK YOU SO MUCH!
@soph506
9 жыл бұрын
This was very helpful and your enthusiasm was wonderful! Thank you
@nataliaorso1649
4 жыл бұрын
I FINALLY understand how TaqMAN works!!!!! Thank you. Loved every bit of the enthusiasm!
@thermofisher
4 жыл бұрын
Thank you! In a perfect world, every scientist working in the field today would still recall that optimism and enthusiasm they had for science when they first discovered it.
@damkhan567
8 жыл бұрын
Great explanation!!! Greetings from The Gambia.
@naglaaidriss328
3 жыл бұрын
Love the explanation fantastic
@riicitos
11 жыл бұрын
thanks for the video, was so easy to understand. Do you have one video to explain gene expression assay by taqman?, that will be very helpfull. THANKS from Ecuador (south America)
@amanyasharon5227
6 жыл бұрын
You have a way of making science interesting, Thanks for the video
@MaimunaK
9 жыл бұрын
This video is extremely helpful, thank you!
@Aerowen19
11 жыл бұрын
you helped me so much. THANKS from germany :)
@MegaBezawit
8 жыл бұрын
thank you!! great presentation!!
@Nissreen-amri
8 жыл бұрын
great ! best explanation found . Thank's a million
@xyz.Axolotl
6 жыл бұрын
Very good video! I thank you as a german student, because I need this for a practical course where we use it and have to write a protocol. Wish there would be more videos how something works. Even your english is clear and easy to understand. :)
@christiann556
4 жыл бұрын
Such a good video! Tnx for the explanation!
@thermofisher
4 жыл бұрын
Our pleasure!
@lawalagboola7694
3 жыл бұрын
Full of energy! Do more videos like this for biotech. You earned my like
@thermofisher
3 жыл бұрын
We will do our best!
@Mona-so9ss
9 жыл бұрын
this is great thanks a lot!!!
@thermofisher
9 жыл бұрын
+daman kaur You're very welcome and thanks for watching!
@fmasx1
8 жыл бұрын
Your videos are awesome.
@buharimuhammad5989
7 жыл бұрын
fantastic
@srijita2070
10 ай бұрын
Understood it really well
@sylr-v_fj5394
4 жыл бұрын
Wow ! a pleasant presentation
@tl8499
4 жыл бұрын
Thank you so much! I'm studying for an exam and I didn't fully understand the principles but the way the information was presented in this video is amazing! I have a better understanding now :)
@iamiuts
6 жыл бұрын
Give that man a promotion!
@Joe-po9xn
6 жыл бұрын
This is an amazing explanarion! I got a nice laugh out of the Pac Man tie in. Very well done!
@dinithividushika3197
6 жыл бұрын
This was really helpful. thank you
@steinhoffski
11 жыл бұрын
Thanks! This is so helpful!
@unicsebastian
3 жыл бұрын
Thanks for this very clear and compact explanation. Just one addition: The "F" in "FRET" stands for "Foerster" (the guy who initially described the phenomenon), not for "fluorescence".
@moshmallow28
6 жыл бұрын
you saved me, thank you!
@sosinaayalew6219
9 жыл бұрын
thank you very much this video helps a lot
@VAFFA
7 жыл бұрын
man youre a legend!!
@suzanap1407
4 жыл бұрын
Perfect video, the explanation is detailed and easy to understand! Thank you!
@thermofisher
4 жыл бұрын
Our pleasure! We're glad you found it so useful!
@qamarhennawi9137
3 жыл бұрын
wow, wonderful explanation thanks
@rakeshchand7549
7 жыл бұрын
I love how this guy explained everything. Thank you sir. Made my assignment a whole lot easier. Can you do a next video on LAMP assay? --Dr.Rakesh Chand from Infectious Disease Centre, Tokyo University of Agriculture and Technology.
@judicedasilvamiguel1393
6 жыл бұрын
Oh man! I would like to be like you...
@Mido55melebari
10 жыл бұрын
great!!
@zahraafakih7020
3 жыл бұрын
thank you loads for the things you are explaining...that's really helpfyul!
@thermofisher
3 жыл бұрын
Happy to help! We enjoy spreading scientific knowledge and insight wherever we can!
@momi1702
5 жыл бұрын
very very useful!!
@thermofisher
5 жыл бұрын
Thanks for watching.
@Navidmsv
4 жыл бұрын
Thank you so much for your invaluable explanation.
@thermofisher
4 жыл бұрын
We're glad to hear it was useful! Thanks for watching.
@AHK1415
3 жыл бұрын
thank you, that was really helpful
@thermofisher
3 жыл бұрын
Thank you for watching!
@mawaddahmusthafa3634
3 жыл бұрын
this is a very helpful video.
@thermofisher
3 жыл бұрын
We're glad you like it! Thanks for giving it a watch.
@naufalhidayat7039
3 жыл бұрын
Thank for your explanation, it very help me to understanding q PCR
@thermofisher
3 жыл бұрын
Our pleasure! Thanks for giving us a look.
@naufalhidayat7039
3 жыл бұрын
@@thermofisher yure welcome
@erkin6586
Жыл бұрын
God bless you 🙏
@1998wiwi
4 жыл бұрын
Joining my fellow grateful students of past, present, and future in this comment section haha, good video, thanks for explaining it so well!
@thermofisher
4 жыл бұрын
Our pleasure! Thank you for taking the time to watch this.
@rtaif5141
2 жыл бұрын
This is amazing thxxxx
@MuhammadUsman-zf5gf
4 жыл бұрын
Well explained Sir
@thermofisher
4 жыл бұрын
Thank you! Hope you found it useful and engaging.
@karamella565
4 жыл бұрын
I love it Thank you
@thermofisher
4 жыл бұрын
We're glad you enjoyed it! Thanks for watching!
@meganversteeg61
5 жыл бұрын
I finally get it now
@thermofisher
5 жыл бұрын
Thanks for watching.
@learnandimplementingit.7971
9 ай бұрын
thank you💌
@duhamardikhi1813
2 жыл бұрын
thank you!
@ouafaoumaima4628
8 жыл бұрын
thank youuuu perfect
@vf84tcat1
6 жыл бұрын
I am a mechanical engineer I am not a biologist. I'm trying to get my head around how PCR can actually benefit mankind. I understand the part about growing strands of DNA. Is PCR purely a biological notion? Or can it do things like build a bridge or produce milk at a faster rate?
@gertrudehemington7570
10 жыл бұрын
In the notes about the Life Technologies TaqMan assays, it is said that primer efficiency can range from 90-110%. How can a primer pair be more than 100% efficient? What does this mean?
@karamdahyaleh5828
8 жыл бұрын
In the case of SNP mapping, a stable duplex forms between the probe and the segment of interest only if the probe is perfectly complimentary to the DNA sequence. If there is a SNP, incomplete binding occurs and the probe is not cleaved. My question is, how does one determine where exactly on the segment of interest is the SNP?
@thermofisher
7 жыл бұрын
Your scenario sounds more like SNP mapping using sequencing. With a TaqMan SNP assay, the genome sequence (and SNP) is known. Two probes are designed to the SNP site, one probe to detect each allele, labeled with either VIC or FAM.
@yanfeng7617
6 жыл бұрын
many thanks!
@thermofisher
6 жыл бұрын
You're welcome, we are glad you liked the video.
@bishakhatwanabasu2667
2 жыл бұрын
So, why do we need both forward and reverse primers, if the probe only attach to one strand?
@nh3028
6 жыл бұрын
you go TaqMan. Get that money
@cinnabarin
9 жыл бұрын
I have a simple question, as a newbie to this technique; In the same reaction environment, how close are these probe molecules and all the cycle products to each other that they do not quench all the freed fluorescent molecules in the vicinity? Afterall, everything is in a single and crowded reaction tube.
@Lovelyfairyheart
11 жыл бұрын
Do you have a video explaining how the allelic discrimination work? By that I mean I'm trying to understand how you take the allelic discrimination plot chart after the PCR instruments spits out the result to understand what your data means. I hope you understand what i'm asking?
@nabilamor
8 жыл бұрын
Great! I would like to know which is the best real time PCR machine?
@jimmyorantes941
6 жыл бұрын
Hi, why do we normalize genes? I do understand that it the housekeeping gene effectively monitors and detects the relative expression of cDNA from sample to sample, allowing us not necessarily use the same amount of concentration when doing qPCR of multiple samples. Can someone go a little more in depth on this? Thank you!
@dr.alialchalabi
3 ай бұрын
how to setup the applied biosystem ONE-step instrument for performing absence/presence (step by step)? please help me to do it from first step of plate setup to last step start run
@dr.alialchalabi
3 ай бұрын
how to setup the applied biosystem ONE-step instrument for performing SNP genotyping (step by step)? please help me to do it from plate setup to start run
@cherrytreez17
7 жыл бұрын
If I amplify a genomic region in a heterozygous species I will potentially have two PCR products in one reaction, but I may not be sure which ones. Is there a way I can pool several designed TaqMan probes (each designed for a certain haplotype, and maybe up to 10-20) to discover what SNPs (and therefore what haplotypes) exist in a certain sample? I suppose each probe would need to fluoresce a different color or intensity. Essentially I am asking if I can use more than two TaqMan probes per reaction to genotype an individual.
@ajhalawani
8 жыл бұрын
Very worthwhile explanation! Is this the same technique for the Ion Quantitation kit that is used to quantify NGS libraries?
@thermofisher
8 жыл бұрын
+Amr Halawani Thanks for the feedback, Amr! To answer your question: Yes. It uses the same technology as TaqMan assays. The probes are specific for Ion Sequencing adaptors.
@naga258
4 жыл бұрын
How do you log the Real time data that you receive from the fluorescent light? And how many units do you have in stock in India?
@thermofisher
4 жыл бұрын
Hi Nagarjun. Thank you for your question. Our imaging system is able to read the fluorescence that is given off of the well and collect the necessary data. It records as fluorescence units. Regarding your units in India question, we recommend that you contact us at thermofisher.com/askaquestion so that your inventory question can be routed to the team in India. Thank you!
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