In this video, I will walk you through the process of analyzing PCR raw data using the delta CT method. By the end of this video, you will be able to confidently analyze your own PCR data and extract valuable insights.
#qRTPCR #qpcr #khanacademy #molecular_biology #biologylectures #genetics
We'll start by explaining what delta CT is and how to calculate it, then we'll show you how to use delta CT to calculate fold changes and analyze gene expression levels. You'll also learn how to use Microsoft Excel to organize and analyze your data.
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Content of this video:
00:00 Intro: Rearrange the data
1:26 Calculate Delta Ct (DCT)
2:11 Calculate Delta Delta Ct (DDCT)
3:50 Calculate fold change (FC)
4:56 visualize the result
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