Just another big thank you from a biochemistry major for all your hard work! You're a talented teacher.
@HA-wn3gg
8 жыл бұрын
you're amazing! I studied my last exam from your videos and I got 17 out of 20, the teacher marked my down 3 marks for 3 questions that I didn't answer correctly because simply you didn't mention it in your videos, all thanks to you I wouldn't pass the test
@KJKP
7 жыл бұрын
Sandwich ELISA starts at 7:08
@MedicoDiscurso
5 жыл бұрын
I appreciate this guy so much. Saving my grade.
@AnnaJeanine
7 жыл бұрын
Thank you for this video! It must have taken a lot of effort to draw the board etc. very very much appreciated!
@JustMe-zz7lw
2 жыл бұрын
Love it. Thank you so much for being a kind person and explaining things for others to understand and not to show off how much smarter you are and rest so stupid. It is a rare quality in teachers to actually want to teach.
@TTHPham
7 жыл бұрын
Very clear explanation and straight to the point. Thank you so much!
@jessym7965
2 жыл бұрын
no worries, I worked really hard T.T.H Pham
@zannatulferdous9087
3 жыл бұрын
The best explanations require no follow up questions. Thank you!
@qamar8655
8 жыл бұрын
OMG I cant thank you enough ♡
@AKLECTURES
8 жыл бұрын
+YulYul Addict You're welcome! :)
@carolinadeluca7242
7 жыл бұрын
Thank you from Belgium! You are very good! PCR, Plasmids, Polyclonal Antibodies, Monoclonal Antibodies, ... all those videos are amazing! I'm not an English speaker and you talk so clear I can understand :) ... Thank you !!
@mirkov6850
7 жыл бұрын
You are a living legend. Respect!
@bhagyasreejm6824
7 жыл бұрын
Lots of thanks .. you always boos our confidence. I always get excited to switch on your lectures
@miriamalvarezramirez5038
8 жыл бұрын
Thank you so much! I loved the way you explained all the concepts
@putritheresiabancin7727
5 жыл бұрын
You're amazing! Thank you for making this simpler.
@linaenger3721
8 жыл бұрын
U did such a good job in explaining this method! Thank you so much for that explanation! (Y)
@astridsiregar
7 жыл бұрын
Omg im so glad i found your channel. Thank you!
@JP-cp1dk
3 жыл бұрын
Absolutely beautiful explanation on Sandwich ELISA! Thank you so much, this will help me with my lab reports! 🥰🥰
@human2951
4 жыл бұрын
Sir sir sir..U r saviour of a biochemist.. Profound Respect from Pakistan...
@princessandbluemoon
8 жыл бұрын
Thank you for uploading this! Very concise and helpful!
@xorg1029
8 жыл бұрын
Just want to say that this video is SO extremely helpful. Please post more.. you explain everything so clearly! THANK YOU!
@anupray9117
5 жыл бұрын
Great!! Very helpful to an Organic Chemist developing Biopharmaceuticals... God Bless You With More Energy!!!
@jolienmangelschots
8 жыл бұрын
Very clear! I didn't understand the Sandwich Elisa really well, but now I do! Thanks!
@MedicSD
7 жыл бұрын
That took a process that I was having difficulty understanding and made it totally understandable. Thank you so much for this and your Western Blotting video!!!!!!! My GPA thanks you as well!
@sydneysimukoko
2 жыл бұрын
Thanks very much Dr, My immunology course is a always easy to understand when I listen from you!
@philipphill9518
7 жыл бұрын
Thank you for this very informative video. There is one thing I noticed though: the image on the bottom right shows the green detection antibody binding to the primary antibody with its Fc region, when actually it would bind with its Fab region. The Fab region is what gives the antibody its specificity against a certain antigen, whereas the Fc region is necessary for recognition by immune cells. Also, primary antibodies binding to two epitopes (antigens) simultaneously is dependent on the size of the epitope and (especially for IgG antibodies) not quite common. I hope I didn't create any confusion. Cheers
@ferrerajones9314
4 жыл бұрын
good catch!
@NikhilGarlapati
2 жыл бұрын
Yeah I was super confused by his drawing, thanks for clarifying! Also would each of the enzyme linked antibodies bind to two separate antibodies of interest because there are two Fab domains?
@summerbreezenight
7 жыл бұрын
I love your videos. They are so clearly explained. Thank you.
@elenaungureanu8919
4 жыл бұрын
Thank you very much for this explicit lesson!
@nevershoutnever80511
5 жыл бұрын
thank you so much all the other videos I watched were so confusing but you made it so understandable!
@woofox333
7 жыл бұрын
saving my gpa like always thank you so much
@Countess_Samar_SprayArt
6 жыл бұрын
This video is just what i needed! thank you
@heidihi547
6 жыл бұрын
Brilliant!! Thank you. So clear.
@sunnykimmy
7 жыл бұрын
You are a gift. Thank you for your awesome, clear, concise and colorful explanations on these methods. :D
@jessym7965
2 жыл бұрын
no you are are gift kim.
@LAisyourgirl
6 жыл бұрын
So clear and concise! Thank you!!!
@sohamkonkar2895
2 жыл бұрын
Killed it bro! Thanks !
@sabasolhmirzaei8591
2 жыл бұрын
Thank you so much for this clear explanation!
@cristinavall8587
6 жыл бұрын
Thank you for your help!!
@monical2648
7 жыл бұрын
the best explanation ever! thank you
@constancapages
3 жыл бұрын
These videos are great! You deserve more views and likes
@musiclover2636
8 жыл бұрын
Thank you so much for your videos!
@nameerahwajahat7494
4 жыл бұрын
i love your lectures so much thank you!!!!
@alysia9151
11 ай бұрын
THANK YOU , THANK YOU , THANK YOU!!! GOD BLESS YOU!!
@okajakifragment4358
5 жыл бұрын
Wow you are such a good explainer and teacher.... Keep up the good work... Kudos to your explanation
@Radosteto0
7 жыл бұрын
I finally got it right! Thanks!
@tiewei1614
4 жыл бұрын
Great lecture series 👍. Very clear. A minor point, in the indirect Elisa, the orientation of the enzyme linked Ab should be the same as that in the sandwich assay. It may be upside down.
@clairewolf6013
7 жыл бұрын
You're seriously underrated! Thank you so much!
@nwilczyn
7 жыл бұрын
the opinion of the scientific community is that he is appropriately rated
@medinlab141
3 жыл бұрын
You are the best! Thank you sooooooo much
@mahmoudalaa323
4 жыл бұрын
you've helped me very much ,, thank you
@julianatoledo3352
3 жыл бұрын
Awesome explanation!
@meghnabose3303
7 жыл бұрын
AK lectures are the best....best explanation to every topic...thank you for making such helpful videos...can't thank you enough😊
@alexaovalles9504
6 жыл бұрын
So good! Thank you so much!!!!
@mayasaba9457
Ай бұрын
Thank you so much for this perfect explanation
@kakossniper
5 жыл бұрын
Why are you such a legend?
@wilfridewilfride5516
2 жыл бұрын
Thank you very much, so well explained and understandable.
@cutielion_bunny1957
7 жыл бұрын
thanks for the explanation helpd me alot !!
@kevindubrow8293
4 жыл бұрын
Excellent. For medical students this is a Godsend
@brittanykaras7998
8 жыл бұрын
I love you. You're videos are so helpful. I wish i could just take your class
@nwilczyn
7 жыл бұрын
he might not be able to wear that sexy athletic-cut v-neck in his class though, so you may be better off!!
@elafalibrahim9828
3 жыл бұрын
Very clear , thank you a lot !
@thevirusdzz1855
6 жыл бұрын
thanks a lot , good explanation ! ^^
@camillakristinekarlsen5358
5 жыл бұрын
Thanks a lot! You are the BEST ever! I just donated:-)
@sainat5174
6 жыл бұрын
Thank you!
@tsgheredagebrehiwet8467
3 жыл бұрын
Excellent lecturer!
@Curious_Human954
5 жыл бұрын
Sir, YOU ARE AMAZING, thank you so much! :)
@user-jy4zj7ep1k
3 жыл бұрын
Watch this on the day of your exam….I see you! Staying up late trying to understand this Keep going!
@MasayoMusic
7 жыл бұрын
Is it possible that when we 'wash' the wells, there is a chance that the bond between antigen-antibody or antibody-antibody w/ enzyme, break apart, giving us a false negative?
@RenaissanceSTL
7 жыл бұрын
Great Job!
@syimirazim4713
6 жыл бұрын
Thank you so much for this very informative video :)
@abdullahbineid4963
7 жыл бұрын
how is the first antigen or antibody we added at the first not removed when we wash off after each step ??
@Dana-rm8cs
Жыл бұрын
Wow ! This was very informative thank you
@rootsm3
2 ай бұрын
Great explanation now it’s clicking
@aihamzamar8329
3 жыл бұрын
one of the best Videos
@astaganaga1906
2 жыл бұрын
Thank you so much!!!!
@IsraeLinoy
8 жыл бұрын
Really helpful, thank you :)
@nareshbarik5384
3 ай бұрын
Thank you ❤
@Silhouette-Oregano
4 жыл бұрын
Thanks very much for this helpful video.
@user-gc4wj9np4f
8 жыл бұрын
thank you !
@ngyongchou3033
3 жыл бұрын
Excuse me, may I ask some question, if for direct detection and direct adsorption of the antigen on the wall of ELISA we still test for the presence of antibody in the blood serum or we are looking for the presence of antigen?
@yewshien580
7 жыл бұрын
thanksss ! very useful and detail explanation
@halani6929
2 жыл бұрын
can the manufuctured antibody, with enzyme(green in n particular) cause change in colour incase a substrate is added to it, just in case the antibody is in its reagent bottle alone?
@charitynkululeko1975
5 жыл бұрын
THANK YOU!!!!!!!!!!
@naveenkrishna5677
7 жыл бұрын
Thanks a lot.. Very very helpful..❤
@dyoomah17
8 жыл бұрын
I spent the whole trying to understand this concept . just after I watched your video everything became clear ,thank you so much
@noorclark2226
5 жыл бұрын
you are amazing, thaaaaaankkkk you
@zainabalkurdi6175
8 жыл бұрын
Just continue Thank you so much
@jessym7965
2 жыл бұрын
i love you AK lectures
@freekruka3705
4 жыл бұрын
this guy rocks
@sondesmohammed8531
Жыл бұрын
You are the best really🦋
@omarahmed-jq9ex
5 жыл бұрын
ربنا يكرمك يا حاج والله 😂❤❤❤
@bustersverden4689
6 жыл бұрын
I love you, man
@ronaldjamatia7119
6 жыл бұрын
thank you sir. may GOD bless u
@sakshikumari4344
9 ай бұрын
Thank you so much it helped me for exams
@journeywithrahat
2 жыл бұрын
Jazak ALLAH sir❤️❤️❤️
@emadnashed5992
11 ай бұрын
Great thanks
@crinklescookies8918
3 жыл бұрын
thank youuuuuuu so muchhhh
@BlackMamba-is8yp
7 жыл бұрын
very helpful and clear as usual one question though I'm not seeing Direct and Competitive ELIZA did I miss it?
@TheMichaelJordanofGambling
7 жыл бұрын
In terms of Indirect ELISA, how does the enzyme linked antibody bind with the antibody that is connected to the antigen? Are these antibodies able to bind through their Fc regions?
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