I don't know how one person has so much expertise in so many difficult subjects. Sir, you are brilliant!
@AbdulazizAlmutlaq
4 жыл бұрын
Eventhough English is my second language, I’ve not faced any difficulties in understanding your lectures! You simplified and made this lecture and the others easy to understand. I can’t thank you enough
@herespecialnothing
8 жыл бұрын
thank you so much you have no idea how much you've helped me. all your videos are great!
@Callmeromain2016
4 жыл бұрын
Your notes are fantastic. If you make a review book with your notes from the board I will totally buy it :)
@lulufonso
7 жыл бұрын
Your explanations are just AWESOME! Congratulations and thank you very much!
@caesar1752
7 жыл бұрын
Again this channel saves me. My text was so confusing but this really straightened it out for me
@beholder9256
8 жыл бұрын
You should make posters of your white boards
@DSD1186
4 жыл бұрын
This guy is awesome ☺️
@a.berruarslan5015
5 жыл бұрын
Great courses! I've learned them for "just a moment" :D
@vevyovi1715
Жыл бұрын
this guy has a unique ability which makes every topic become easier to understand
@junczhang
8 жыл бұрын
love your lectures! thank you!
@abrarabdullah1352
3 ай бұрын
Sir, you are a G! I will literally pass my subjects because of you. May you be blessed
@eleonora7743
7 жыл бұрын
So clear, thank you for saving me!
@stronger3381
5 жыл бұрын
Great work! Keep striving!
@mohammadzareefabtaheeakhan6708
3 жыл бұрын
you explained everything what my professor taught in 2 hours!!
@femafull
6 жыл бұрын
Thank you so much for doing this! I understood it perfectly !
@johnbarba851
7 жыл бұрын
Exceptional Work as always ! Thank you !
@abeer369.14
5 жыл бұрын
Definitely the best video to explain western bloting
@DaFan86
7 жыл бұрын
You are absolutely amazing, thank you!
@ThanakritGu
6 жыл бұрын
Thank you! This was very clear and concise!
@filzaamara6205
3 жыл бұрын
bro this is so clear, i understand it immediately. thanks!
@kiplagatenai
7 жыл бұрын
Thank you for this, helped me a lot!
@Smacy
2 жыл бұрын
Seriously your videos are like the only KZitem lectures I even watch anymore lol
@LA-cm9uo
3 жыл бұрын
Thanks bro, you explain better than all of my professors combined.
@laurencooper2698
4 жыл бұрын
THIS IS AWESOME! thank you so much!
@kbgeshji9385
4 жыл бұрын
i call u the 'operation sir' the energy u exert is really contagious , best conyer, communicater,
@bodooryaseen839
7 жыл бұрын
Very informative ! thanks for the efforts
@sean787222000
5 жыл бұрын
very clear lecture, you are the best!
@irtizakhan7456
2 жыл бұрын
leave me speachless every video
@entropyinreverse
7 жыл бұрын
Thanks for all your help, keep up the good work!
@AKLECTURES
7 жыл бұрын
you're welcome! will do!
@jiaronglin8693
8 жыл бұрын
Clear explanation! thank you!!
@paul7533
8 жыл бұрын
Thanks alot. Your videos are really good.
@selmam9676
5 жыл бұрын
You are just amazing ! Thank you so much !
@dakshvelu
3 жыл бұрын
Thanks for this video..! Perfect n upto the point💯
@vladghies3002
2 жыл бұрын
Brilliant explanation!
@DA-sj2gw
6 жыл бұрын
What do you do after you isolated the protein in the last step?
@vamshipillarishetti
6 жыл бұрын
Perfect!! Thank you.
@DiegoDiego1989
8 жыл бұрын
Woooow Best video on Western Blotting. teck you man. help me a lot.
@manucene
7 жыл бұрын
Amazing clarity.
@bartpellegrini3478
3 жыл бұрын
Best explanation ever. Thanks man
@jeppeflner5280
6 жыл бұрын
I am a little confused about something: If you see a western blot, containing multiple bands, does that mean that you have created specific antibodies for each of the proteins that make up each band? I mean, in the above example, only one band is visible after the x-ray, the band containing the protein to which the specific antibody would bind. That must mean, that if you have multiple bands that are visible on a western blot, there has been made specific antibodies for each of the bands, right?
@maryammd7
3 жыл бұрын
Thank you!
@esmartiz3093
5 жыл бұрын
very nicely explained. Thank you :)
@faithoyamendan9244
5 жыл бұрын
You are a genius! Godbless you! Well understood
@qamarhennawi9137
2 жыл бұрын
You are the best!!!!
@TheTheaterThug
6 жыл бұрын
But how could you find the concentration of the antigen /POI then after you isolated it?
@Chelssums
3 жыл бұрын
thank you!
@inespotzlberger1450
3 ай бұрын
it took me so long to understand the single steps and you explained it so easy with everey info i needed. But i have one question: Why not mark the fist Antiboddy with Radioaktiv Isotops? What would be the differen?
@ASHTUTORIAL
6 жыл бұрын
Thanks for this video
@inesgonzalez2246
2 жыл бұрын
Awesome lecture 👌
@Hashpotato
3 жыл бұрын
what a boss. brilliant explanation
@dddd___77
3 жыл бұрын
You are absolutely amazing ♥ thank you
@jesseshooter4403
8 жыл бұрын
What's the purpose of using two antibodies? Wouldn't it be simpler to just use a single antibody that is radioactivity labeled?
@nahidkhan1234
7 жыл бұрын
Thank you, sir
@hotsummer4567
8 жыл бұрын
Excellent I owe you dude
@dllkss
8 жыл бұрын
What about blocking the membrane to prevent antibodies from binding non-specific to it?
@SforScience
7 жыл бұрын
yes thats a good point we have to block the membrane for undesirable binding using a milk that blocks non specific interaction
@vil4332
6 жыл бұрын
Brilliant explanation. Change the speed to 1.5x to save time though.
@ginavallefuoco4428
5 жыл бұрын
I love you Thank you Take the money I give to my University, you deserve it more
@alphagonist4748
3 жыл бұрын
Why can't we directly use radiolabled antibody
@ka1458
3 жыл бұрын
hello, why cant we make the primary antibody radioactive? so no need for the secondary. thank you!
@JuSt2MaN
5 жыл бұрын
you are a legend
@noahhubscher2926
5 жыл бұрын
good job thanks.
@deepacavalli-sforza1886
7 жыл бұрын
excellent !
@abdirashidadan8896
7 жыл бұрын
hellow, Mr. Science , i only wanna say that u helped me a lot. because it s unfair to to listen your lecture and not to thank you once. i hope you will introduce us. Molecular marker in lecture coming. thanks for you service to the human
@areejaroma609
8 жыл бұрын
thank you so much
@mugwanyamuzril1131
7 жыл бұрын
I don't think proteins on an SDS polyacylamide gel will move according to charge because the SDS makes all amino acids of the protein to acquire a uniform charge besides protein segregation. so proteins will separate according to size
@Mrjmaxted0291
5 жыл бұрын
Isn't this covered on a 2D electrophoresis gel where it has a pH gradient to differentiate compounds by their charge via isoelectric focusing?
@seyramm.duphey2248
5 жыл бұрын
Shouldn’t another protein eg BSA be used to cover all uncovered spaces on the membrane?
@MrChadRB
5 жыл бұрын
He meant they move due to the charge imparted on them in the electric field. He specifies that their position is only dependent on their size.
@lunardifranciele
7 жыл бұрын
Perfect!!!
@jackqiao6406
7 жыл бұрын
From what I can tell, western blotting protocol is a combination of PAGE and ELISA. Please correct me if I am wrong on that part, but to identify a specific protein can't we just use ELISA instead of western blotting? I probably sound silly asking this, but I am really confused.
@enacte
3 жыл бұрын
Wouldn't denaturing the proteins make the monoclonal aB no longer specific?
@buriku9541
7 жыл бұрын
thank you :)
@sabrinaflask1617
7 жыл бұрын
Can anyone explain to me why you cannot visualize a protein using the primary antibody conjugated to a fluorescent molecule?
@SforScience
7 жыл бұрын
the reason is sensitivity and signal amplification ..... if the desired protein conc is very less in western blotting than a few of the primary antibody can bind and giving a less signal ..... so multiple secondary antibody can bind to the primary antibody as a result signal will be amplified and we can detect protein even in the less concentration which increases the sensitivity of the process... hope u liked ,y ans subscribe my channel and post more question and i have lots of videos coming up for u guyzz
@nahidulislam2408
5 жыл бұрын
Thank you sir
@mahdihurreh8836
5 жыл бұрын
oh ma gawd this guys amazing
@charlottechan8624
3 жыл бұрын
thank you good sir
@subhasaryal8122
8 жыл бұрын
nice one ,,helpful
@romanbarankov2833
Жыл бұрын
Why do we need a secondary antibody? Why can't we label the first antibody radioactively?
@evangelinagarcia9336
2 жыл бұрын
How come the primary antibody isn’t radioactive that way we don’t need a secondary antibody?
@vistian
2 жыл бұрын
Why not just radiolable the primary protein and skip step #5 altogether?
@user-ro1ko7nw5e
6 жыл бұрын
Thank you for the lecture. One question: when should we use ELISA and when should we use Western blot?
@alx4081
6 жыл бұрын
You use ELISA first bc it's less expensive and if it turns out positive you check with a western blot which is more expensive but 100% valid
@zandelion9011
6 жыл бұрын
Need more microbiology based videos..
@user-qc3uv8bu8b
3 жыл бұрын
love from egypt
@marwaobiedallah8839
8 жыл бұрын
More than Excellent
@shaboatrad4066
7 жыл бұрын
How do we make the monoclonal anti body specifically for our pr that we want to study!?
@SforScience
7 жыл бұрын
Jus take ur protein, inject in to mouse , a few days after take out their spleen and u will ur antibody specific for that particular protein
@igormenezes6634
8 жыл бұрын
Could you Please Add some translation to portuguese
@anythinggoes4588
7 жыл бұрын
You should teach my lecturer, 'Mr Giving Out Handouts' lol
@nehakhalid8910
2 жыл бұрын
Thank u angle😂😍
@hanibarnieh2789
4 жыл бұрын
I LOVE YOU
@victornguyen8382
4 жыл бұрын
Godsend
@dreamdiction
2 жыл бұрын
The pandemic only exists on TV, not in any hospital.
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