The Most Highly Cited Paper in Publishing History: Protein Determination by Oliver H Lowry: www.jbc.org/article/S0021-9258(20)56859-2/fulltext
@MridulKaimal
2 жыл бұрын
a bit more explanation on why and what makes the specific absorbance for nucleic acids at 280 would be useful. thanks. otherwise lovely tut
@adwoabiotech
2 жыл бұрын
Noted. The Tryptophan and tyrosine amino acid residues allow absorbance at ~280nm. Thanks for dropping by and wish you the very best with your studies/work.
@Vkings1
Жыл бұрын
Thank you so much
@alhassanmohammedawal3851
7 ай бұрын
Hi Maame Adwoa, please i have a malaria vaccine to analyse the protein content. I have the Bradford Protein kit from thermofisher and the Albumin standards for the curve. can you help me with how to go about it?
@adwoabiotech
7 ай бұрын
Hello Alhassan, You will need make a 6-point serial dilution of a protein standard for which you know the starting concentration. e.g. Bovine Serum Albumin (BSA). You will then measure the absorbance of that standard against your protein of interest, in this case, your vaccine. If you have a plate reader, it may be also be easier assay it with that instrument (provided you have enough reagent for the required plate reader vol., typically 200ul). I have a video on here, that discusses how to approach protein determination so I'll link it below.
@adwoabiotech
7 ай бұрын
Standard Curve: kzitem.info/news/bejne/roaH2ml6np-rnYI
@alhassanmohammedawal3851
7 ай бұрын
i tried to contact them but they will not response
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