Nice video ❤ also The screening technique destroys the cells so isn't it counter-productive? We want to clone the gene of interest and now we destroyed the cells that are going to do so or is there another step after the screening part ?
@thebumblingbiochemist
4 ай бұрын
Thanks! Screening doesn't destroy the cells, so I'm not sure what you're referring to? Can you clarify?
@christopherleubner6633
3 ай бұрын
The screening reagent us an antibiotic, typically you would insert a gene coding for antibiotic resistance along with your target gene. Then you are able to select the modified bacteria by using an antibiotic to get rid of the non modified bacteria. Another technique is to add a florescent protein gene and select the colonies that glow.❤
@thebumblingbiochemist
3 ай бұрын
Correct! Using the antibiotics is an example of a selection technique, whereas fluorescence would be screening.
@I_am-.-Sam777
Жыл бұрын
I'm a new master student about molecular biology, your videos 're helpful. Thank you so much. Wish you all the best!
@jeremiahlittle2271
Жыл бұрын
I'm interning at Biochemistry lab and know very little! Your videos help me play catch up! Thank you!!
@thebumblingbiochemist
Жыл бұрын
Happy I could help! Good luck with the internship!
@anacano8685
Жыл бұрын
Thank you so much for this content! It was really helpful 💙💙💙
@thebumblingbiochemist
Жыл бұрын
So great to hear!
@sholeh_zard
Жыл бұрын
girl thank you so much for making this video! this is so good quality and free 🥺 thanks for giving me some core info to be able to start my assignment 💛💛💛
@thebumblingbiochemist
Жыл бұрын
happy I could help! Good luck!
@olgavolchansky4372
Жыл бұрын
Hi! This is so informative and so easy to follow-and exactly what I needed- thank you!
@thebumblingbiochemist
Жыл бұрын
That's great to hear - thank you! So happy I could help
@youngwang4605
Жыл бұрын
Great video Brianna 👏, but I still have a confusion~~ After completing SLIC, three plasmid types are present in our samples, right? The template plasmid (produced by bacteria and modified by methylation), the plasmid containing the 'insert' (unmethylated), and the plasmid containing the site-directed mutagenesis. So my question is: Why did site-directed mutagenesis would happen in SLIC? Is the plasmid containing this mutation a byproduct of PCR in SLIC? As a PCR product if it is not methylated it cannot be degraded by Dpnl, how do we remove it? 🤔
@thebumblingbiochemist
Жыл бұрын
Thanks! You only have a single intact plasmid for each PCR reaction, and then you DpnI it. None of the PCR products should contain the non-mutagenized region.
@ManitaRaina
Жыл бұрын
Nice video👍informative and helpful. I have a question, I need to clone recombinant protein vector. For that I have ordered primer made from cds sequence of the gene of Interest. These primers have forward and reverse sequence along with that restriction enzyme site. My forward primer also has kozak sequence and flag tag. I prepared cDNA of the gene and put it for pcr with these primers but its not at all amplifying. I even used control cDNA and primers from takaras multiple tissue cDNA kit as positive control and kit control primers with my cDNA for troubleshooting, but no bands came. Can you suggest any method or literature which I can refer?
@thebumblingbiochemist
Жыл бұрын
thanks! sorry you’re having difficulty with your cloning. are you trying to amplify out of cells?
@s1va3209
Ай бұрын
Awesome finding your videos. Will watch all of them.
@thebumblingbiochemist
Ай бұрын
Glad you like them!
@g_ayugane
Жыл бұрын
i have a biochem exam coming up in two days, and this video was really helpful. Wish me luck!😊
@thebumblingbiochemist
Жыл бұрын
Hope it went well!
@rainlin702
7 ай бұрын
How great the video is. It made me more familiar with cloning knowledge!
@thebumblingbiochemist
7 ай бұрын
Glad it was helpful!
@alicehayward8214
Жыл бұрын
this helped me so much for my lab work. thank you!
@lou7572
6 ай бұрын
Wow! You are so good! I wish you a great career in science!
@thebumblingbiochemist
6 ай бұрын
Thank you!
@levantrinh3412
11 ай бұрын
So nice 😂
@BrainQueen1
2 жыл бұрын
Thank you so much for the great video! I am wondering, when you do PCR cloning and use primers that contain no phosphate groups but your vectors also don't have phosphate groups because you might have used AP (alkaline phosphatase) to remove them because you don't want self-ligation to occur, you use PNK to add phosphate groups. But how do you control adding phosphate groups only to the primers and not to the inserts and not to the vectors when they in the same mixture?
@BrainQueen1
2 жыл бұрын
Correction: But how do you control adding phosphate groups only to the inserts and not to the vectors when they in the same mixture?
@thebumblingbiochemist
2 жыл бұрын
Thanks! You would do the modifications before mixing them
@صالحطليان-ف8ل
8 ай бұрын
Thanks you my sister from yemen
@thebumblingbiochemist
8 ай бұрын
So glad I could help!
@dgoodall6468
Жыл бұрын
Excellent video thanks!
@thebumblingbiochemist
Жыл бұрын
Thanks!
@peterbasaking6607
Жыл бұрын
good job💯
@thebumblingbiochemist
Жыл бұрын
Thanks!
@brainandrain3848
2 жыл бұрын
Great video and explanation! 😃
@thebumblingbiochemist
2 жыл бұрын
Thank you!
@peachyeinna
Жыл бұрын
thank you so much for this incredibly helpful video! i'm currently an undergrad and was so confused when my PI explained it to me. I clicked on your channel and to my surprise, there are hundreds of other videos like this one. I feel like I struck a goldmine haha. I appreciate you!! 🫶🫶
@thebumblingbiochemist
Жыл бұрын
Thank you so much! I'm sincerely super happy to know you found this (and others) helpful. Best of luck with your studies!
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