I use a blue led flashlight to shine through LC. The short wavelength makes cloudiness really apparent.
@FreshfromtheFarmFungi
2 жыл бұрын
cool nice tip!
@Sgtspork
2 жыл бұрын
interesting, will check that out.. was gonna build a little square led light table.. that you can sit a LC jar on or an agar plate.. using one of those internet controlled variable color led bulbs, so I can cycle through various wavelengths.. it was just half an idea.. building out from existing similar light boxes I've seen others use, just my addition was the variable color bulb, tho I'm sure I'm not the first to think of it... tho I had seen somewhere, mossy creek I think, had a vid where he used a green pen laser to inspect LC cultures that worked well... but this sounds like it confirms that different light wavelengths will help a bunch.. awesome! thanks!
@squirlmy
2 жыл бұрын
wait a second... if there's doubt about whether the cloudiness is from bacteria or perhaps a chemical reaction, for example, honey started to caramelize, but broke up for whatever reason, instead of clumping. Maybe that's not a great example, but would the blue led test actually differentiate bacteria from dispersed sugars? This is even a concern of mine about the "turbidity test" in the video. I want to be sure there's something more reliable than taking some random person off the street to tell me "Yup, looks cloudy!"
@julescircuits845
Жыл бұрын
@@squirlmy Its just a quick "indicator". The only real test is to take some out of the 3D environment and inoculate a 2D surface I.E, an agar plate to see what's actually growing on it. Further to that an inspection under a microscope if you want to go to extremes. Hope that puts perspective on it :)
@smokey689
Жыл бұрын
I made some LC that was clear an i used a blue led. You could not see that it was contaminated. When you put it on agar it was totally contaminated
@Sgtspork
2 жыл бұрын
I tried the needle biopsy method for cloning a fresh mushroom sample to LC recently (which I saw on an earlier video you made), where you draw up a few CC's of sterile water into a syringe with a sterile large gauge needle attached and use the tip to take interior flesh samples from a fresh fruit as you would for cloning to agar (I actually did both for redundancy safety). And it worked beautifully.. I have a nice thick mycelial mass in my quart LC jar consisting of a 4% karo/distilled water solution with just under a gram of bacterial peptones added. No sign of contaminates two weeks on now, tho I do still need to do an agar plate to confirm.. Thanks for that! I know it can still be a risk but as long as I still do the agar plate transfers as back up.. no time or sample lost.. only win if it works..
@FreshfromtheFarmFungi
2 жыл бұрын
I would still verify on agar, sometimes they can hide in the mycelium but from my experience you should be good to go forward if it doesn’t look turbid or smell funky 👍🙂❤️🍄
@Sgtspork
2 жыл бұрын
@@FreshfromtheFarmFungi oh yeah I always QC on agar a few days before any inoculation.. good advice. my general rule when it comes to LC is to wait a week before inoculation.. a few days for anything to grow that the PC might have missed, then take a sample or two and QC it on an agar plate or two.. wait a few days to see if any bacteria colonies develop.. if none, then it's good to get inoculation. but then I also QC well after inoculation, a few days before using it on grain spawn... the cloudiness is a good indicator of course but only if the contaminate is widespread thru the LC medium, because as you said, with say a partial contamination, there can be colonies hiding among the mycelium that are hard to distinguish visually, especially since it's a 3d medium and not 2d like agar.. all just as a matter of my usual technique and routine.. it's saved me a ton of time and effort, all for just taking a moment to do that... it takes no time at all to take a sample and streak an agar plate or two and just a few days to see results.. when if you do a 5lb grain spawn bag, it may take weeks for a bag to show visible contamination issues only to get discarded.. and usually you used the same batch for several bags... better safe and a little wait than weeks of colonization only to end in failure.. I have yet to attempt this with a spore syringe directly, currently, I generally do many agar plates and to a few grain jars of the leftover spore solution.. and if the grain jars do not produce contam and survive to full colonization, I will do a small shoebox grow just to see what interesting fruits it produces that I might be able to clone from it.. while also doing the regular work with the agar.. the grain jars become a sort of "back up" to the agar, but also a way to experiment with the strain as well.. and it's helped me produce some good results actually with some mutated cross strains I've worked with in promoting certain variable characteristics in those lab created strains.. (A.P.E.R., P.E. 6 for example). I may experiment now with LC as well for that, because hell, why not? sounds like fun.. :) cheers and keep up the great work!
@NetsarimTheWatchman
11 ай бұрын
I use this exact method to test and it works beautifully. I have been cussed out and argued with online with people saying this isnt good and I need to streak test on AGAR. This saves material and time. So theres that. Not to say I havent had a turbid jar full of contamination. I just didnt use it. Great video!
@madmaxofspokane1691
10 ай бұрын
I appreciate this video. I did a grainspawn to quart LC solution of Amanita Muscaria. It took of lightning fast at reproducing. I had over 300ml of 600ml LC in 72 hours. At that time I carefully took a sample of what looked like clean mycelium & transferred to another quart LC solution. Now in the 1st jar, I've been putting it on the magnetic stirrer daily to keep breaking up the long strands of mycelium. The LC solution is so thick after it settles that the whole mass is impossible to even see a bright flaslight shine through. But I can clearly see through the solution with both my little flashlight\black light, and read text on both LC jars. So now I can put to rest all of the social media haters that have been telling me to throw it all out, it's completely contaminated.🍄🍄🍄🍄🍄🍄🍄🍄
@MrHavNaught
9 ай бұрын
Nice I got amanita culture syringe thinking about shooting in lc jar, just not sure how to grow them bcuz they're mycorrhizal and grow with trees, what you got planned I don't want to waste it, thanks
@Turntuppusha
8 ай бұрын
Stop tryna spin it leave it alone . Now it’s done for you stalled it out dog . I’m no master by any means I learned from an OG who was not a nice dude . They all lies and cheats at a personal level . Many mushroom growing narcs out here it’s insane 😂
@Gorkilein
2 жыл бұрын
With liquid culture I always go fron syringe directly into the LC, since I dont have a flow hood. It takes some time but always worked perfectly! Inever had contamination that way. If you heated the needle and whiped the container injection port with Alcohol then the bacteria were in the syringe already.
@zuul902
2 жыл бұрын
the bacteria is in with the spores... spores should always go to agar first.
@Gorkilein
2 жыл бұрын
@@zuul902 when you do your syringe yourself you could avoid it (or see it in the syringe as in the LC). The ones I bought never were contaminated by now.
@ericsankey5756
9 ай бұрын
Your videos are great bro, thanks for the info!
@SpecialistGuava
10 ай бұрын
gary you are an absolute gift to the mycology community
@youtubedeletedmynamewhybother
8 ай бұрын
If understand correctly. Bacteria will rapidly colonize the entire mass of water where as Mycelium will clump and sink. I just made my first LC jar the other day, i went 0.7 grams heavy on the organic honey so the water has color. But it definitely still passes a turbidity test and the mycelium seems to be aggressively colonizing it which is a good sign.
@canberradogfarts
2 жыл бұрын
I just wanted you to know you have inspired me to build my first lab, from scratch. I have an 8x10 room bare, to build out. Ive got the materials to build my horiz lam flow, lights, three wire kitchen racks, and room filtration. Gonna air seal it to 15"wc, double door air lock. Got a stainless "bar sink" 12" sq. A microwave and a single eye induction cooker. These will be the "media production corner." The room will have a 1 volume per hour exchange rate thru MERV 14, three stage air handler with room for future cooling coils. I currently plan on using a split AC, 2500 btu to cool with in floor radiant heat. The insulation wrap will be R50. Quite quiet, filtered and thermally stable. My budget for the room is $2000, that incudes basic outfitting. There will be a grow room immeadiatly adjacent with grow tents scattered throughout the bldg. I just want to thank you personally for the inspiration to build my own lab.
@FreshfromtheFarmFungi
2 жыл бұрын
sounds like a nice setup! save some $ for some decent glassware and you will be set to go 👍❤️🍄
@Zayskibop
2 жыл бұрын
Immaculate inoc. still makes me laugh 😂
@FreshfromtheFarmFungi
2 жыл бұрын
they will be holy 🙌😄
@canberradogfarts
2 жыл бұрын
I know, right! As if lil baby Hey Zeus was a fungus.
@donniebrasco11
Жыл бұрын
Hey I liked your podcast with geeky mush love brother :)
@FreshfromtheFarmFungi
Жыл бұрын
thanks man! That was a fun convo I love doing those! 🙏🏻🍄❤️
@shainemaine1268
2 жыл бұрын
Loving those PP5 lids! No more rust...
@FreshfromtheFarmFungi
2 жыл бұрын
yes they are nice and have a good seal on the jars too
@harshdholariya2507
2 жыл бұрын
How to we know liquid culture are fresh or contaminated ??? And ready for inoculation on agar plate ? How to check it
@squirlmy
2 жыл бұрын
well, inoculation on agar IS my test for bacteria. Maybe you are doing microscopy? But, if I were not testing, I wouldn't use agar at all. It would go straight from Liquid Culture to grain. I'm not sure what else you plan to do with the inoculated agar.
@zuul902
2 жыл бұрын
Hi! Instead making 4 more LCs, why not just place a small bit on agar and try and transfer to another plate or two to get some clean growth and then inoculate 1 LC with a clean agar wedge? Is it easier to do it your way?
@FreshfromtheFarmFungi
2 жыл бұрын
It could be done but sometimes its hard to isolate clean mycelium especially from motile bacteria that can hide in the colony so I just usually start from scratch
@ericsumner9859
2 жыл бұрын
Hey Gary. Great content. Love your channel. Do you ever see the mycelium grow on the jar glass in the solution? It’s my first time and seeing lots of growth on the sides of the jar in the substrate as well as swimmers. No turbidity
@FreshfromtheFarmFungi
2 жыл бұрын
yes this usually happens like a “scoby” with more rigorous strains 👍 should be ok if it remains sterile
@julescircuits845
Жыл бұрын
Gary, quick question... Is it possible to grow out haploids in a small jar of LC or do only diploids do well? Say If I have found a strong haploid I'd like to breed with other haploids? Ever tried it?
@FreshfromtheFarmFungi
Жыл бұрын
yes it should work - it may eventually fizzle out faster than normal but it should be a viable way to store them
@julescircuits845
Жыл бұрын
@@FreshfromtheFarmFungi Thanks so much for the help :) I hope that both your new son and farm are doing well! Have a good Christmas too hehe
@BadddDoggg-id4po
5 ай бұрын
I've never seen a pro go from spores to liquid culture or grain. All spores are contaminated to some degree and you need agar first to separate out the contamination.
@FreshfromtheFarmFungi
5 ай бұрын
not if the mushrooms were grown sterile in vitro
@erkyderky
2 жыл бұрын
Out of nowhere I’m having very bad wet rot. Don’t know if it’s my LC or my spawn
@shuckahoseerazzle8486
Жыл бұрын
might of put to much lc and having to much liquid for your spawn to handle
@kingsbing5483
Жыл бұрын
When u add nutrients its cloudy right out of the pressure cooker
@FreshfromtheFarmFungi
Жыл бұрын
try getting lab grade or filtered media it should not be cloudy
@RomanHold
Жыл бұрын
It's gonna leave out the "select genetics - clone favorite" part and we gonna have a liquid culture with lots of empty spots in the canopy, cos the genes are all over the place.
@ScottWConvid19
Жыл бұрын
My cultures were sort of cloudy from the onset. I think it's because I didn't boil the water it heat up my mixture and I used a recommended recipe of malt extract, nutritional yeast, bone peptone (I had not known whether bone or soy would be best), and gypsum. This recipe was touted to be good to strengthen mycelium growth as well as inhibit the growth of any contaminants. Now I'm not sure. I did a test drop off each LC into agar plates and now I'm waiting for the results, but in the beginning, my mycelium took off! Then it began to wane and now it looks either stunted or possibly even dying 😢
@davidwilliams9997
Жыл бұрын
I just had the exact same experience. Used peptone and light malt extract. LC looked great, started reproducing really fast, then got cloudy and nasty looking on the bottom. Disappointed after the great start.
@ScottWConvid19
Жыл бұрын
@@davidwilliams9997 5 out of 7 jars were toast. I think it was a bacterial contam.
@davidwilliams9997
Жыл бұрын
I’m working on some more this very minute. Going back to what works best for me, Karo & H2O. Good luck friend.
@cestlaviehappner1872
Жыл бұрын
He'll question can you do a video on cleaning jars how to clean the soap scum and another question I found a species of mushroom and I'm pretty sure you've grown this mushroom before it's called this silky rosegill and I'm going to be trying to transfer fresh tissue to agar I'm going to try it on in agar malt extract with using Brown rice water mixed with wheatberry water what do you think you think of the chances
@basilroland
11 ай бұрын
A BACTERIAL COLONY IN WATER MIGHT GROW QUICK BUT WILL CRASH ALSO QUITE QUICKLY. IF YOU WAIT FOR A BIT , YOU MIGHT HAVE A USABLE CULTURE WITH HEALTHY MYCELLIUM. sorry for the caps
@basilroland
11 ай бұрын
depending on the stain of bacteria that you had
@FreshfromtheFarmFungi
10 ай бұрын
this might be true but I recommend using sterile media as it will be more reproducible over time. Unless these companion bacteria can be identified and replicated it’s taking a big chance that they aren’t harmful.
@joaopedroalves7507
4 ай бұрын
my liquid culture always remains cloudy even without inoculating. I dont understand😢
@FreshfromtheFarmFungi
3 ай бұрын
it could be the quality of your media - try to get lab grade and see if that helps (Or use filtered honey)
@Wavy_Gravy
Жыл бұрын
Currently attempting a multi spore LC from a syringe from a vendor that's reputable for cleanliness.......fingers crossed lol.
@FreshfromtheFarmFungi
Жыл бұрын
if it fails and you didn’t use it all try diluting it more and trying again this seems to help
@Wavy_Gravy
Жыл бұрын
@@FreshfromtheFarmFungi Really now 🤔 you sir are a saint thank you.
@chascodelviso
Жыл бұрын
I would pass that liquid culture to an agar and then try and isolate want you want, then pass that to a liquid culture.
@FreshfromtheFarmFungi
Жыл бұрын
this is a good technique
@rg.milkman8056
Жыл бұрын
I dont understand why people are saying using spore syringe is risky?? if i have a brand new sterile syringe?
@FreshfromtheFarmFungi
Жыл бұрын
if you do it yourself and the spores are collected in vitro aseptically then yes. But there are many unreliable vendors who will not do this and blame the users so it’s best to cover all bases and do agar.
@fredbrighton9972
2 жыл бұрын
Hi. Don't you think spores in LC will create a multi strain mycelium ? Isn't it better to do a biopsy in LC and this way, create a clone ? Competition won't exist with a clone... With spores, it will be random don't you think ?
@FreshfromtheFarmFungi
2 жыл бұрын
yes look up our hyperbreeding video it works. kzitem.info/news/bejne/wK5m2nyVrIVhpaw
@ishomehome
11 ай бұрын
Have you ever thought of getting a Portable Turbidimeter To test LC?
@FreshfromtheFarmFungi
11 ай бұрын
yes maybe if I find a good deal
@ishomehome
11 ай бұрын
@FreshfromtheFarmFungi Hey, I can't get rid of fruit flies. I have fly traps, vinegar quort containers. I keep almost whole harvests because they contimate with white mold that looks almost like it's pinning. Any thoughts?
@ishomehome
11 ай бұрын
@@FreshfromtheFarmFungi almost lose not keep
@HeyRaeSunnyDay
2 жыл бұрын
Awesome video, Garry! Questions: do you have to have FAE port on jar lids for LC? Or, can an unmodified lid be OK?
@FreshfromtheFarmFungi
2 жыл бұрын
you don’t have to have it but it definitely makes things easier and allows the mycelium to breathe and grow faster
@asapeterson2751
5 ай бұрын
the more pda that is in your culture, the less visible the letters would be. unless your using only honey or corn syrup. I feel like u havent considered this
@jerseyhovidea
Жыл бұрын
So what can I do when my LC is bacteria positive on a plate and I dont have original spores any longer? Is there a way how to save the culture? Maybe some streaking?
@FreshfromtheFarmFungi
Жыл бұрын
yes you can do many things, watch this one kzitem.info/news/bejne/lpub3odjhKF2gKw
@jerseyhovidea
Жыл бұрын
@@FreshfromtheFarmFungi thank you for such a fast reply!!!
@joshbell4176
2 жыл бұрын
What kind of jars are those?
@rafals5113
2 жыл бұрын
Is this syringe filter are necessery in liquid culture? I noticed that some people dont use it, only injection port.
@FreshfromtheFarmFungi
2 жыл бұрын
it isn’t necessary but helps when you inoculate and draw up cultures because of the pressure displacement
@dhaval4570
2 жыл бұрын
Mine goes a little cloudy as I shake it, but it gets clear after the myc settles like in 30 seconds. Is this normal ? Using grain water
@FreshfromtheFarmFungi
2 жыл бұрын
yes if it’s unfiltered water it’s probably debris
@dhaval4570
2 жыл бұрын
@@FreshfromtheFarmFungi placed it on plates just to be sure :)
@pauloantunes8826
2 жыл бұрын
~Hi Gary!!!! How mush LC do you use per Kg or L of grain? Sorry I am from europe ;)
@FreshfromtheFarmFungi
2 жыл бұрын
I use about 2cc for a half pint jar or 10cc for a 5lb bag ( 5 lbs = 2.28 kg)
@buddhaalphahealingsounds369
2 ай бұрын
What’s your LC recipe?
@FreshfromtheFarmFungi
2 ай бұрын
5% honey water
@DrLouCav
Жыл бұрын
What if there is a floater on top? Can that be contamination ?
@FreshfromtheFarmFungi
Жыл бұрын
It can but it doesn’t mean that it is, often times they will grow on top like on a petri dish
@DrLouCav
Жыл бұрын
@@FreshfromtheFarmFungi thank you the jar is not turbid but has a round growth on the top
@Lulzswag
2 жыл бұрын
What recipe/amounts do you use for your liquid cultures?
@FreshfromtheFarmFungi
2 жыл бұрын
4% honey water
@Sgtspork
2 жыл бұрын
I use a 4% Karo/distilled water (as my local big city water is too hard and not great) with .5 to 1 gram of bacterial peptones (from Amazon).. tho I might switch to a good raw filtered honey (I think he's using a raw local honey, it is definitely filtered and local, I know that for sure, just unsure if it's raw or not.). I would think, and this is anecdotal and not scientific at all, it would have more micronutrients perhaps coming from such a natural source from Karo.. you don't wanna go over 4% tho, as more sugar becomes too toxic to the myc.. using Karo or Honey is also good due to the clarity it gives to the medium over light malt extract which can get residual caramelized particles and such if not completely dissolved, especially after PCing. due to the high heat.. both Karo and honey give a clear medium to work with.. no straining required either.. I add the Peptones as its a nutrient source for Nitrogen and a host of other stuff and greatly aids in growth (more than anything I've seen by a huge factor). I also use it in my agar formulations. also, here's the quick equation to figure out what you need to add for a certain percentage of solution... grams of solution = (wt% of solution) x (ml of water) / (100-wt% of solution) so if your target is say a 4% honey solution in water... that would look like... (4x500ml)/(100-4%)=20.83g 2000/96=20.83g add 20.83 grams of honey to the 500ml (500g) of water.. to create a 4% honey/water solution. hope that helps!
@looneycrow7978
Жыл бұрын
You can still rescue LC with agar cant you?
@FreshfromtheFarmFungi
Жыл бұрын
yes but I have clean slants to go back to
@MrBazz420
2 жыл бұрын
whats your liquid media recipe?
@FreshfromtheFarmFungi
2 жыл бұрын
4% filtered honey water
@MrBazz420
2 жыл бұрын
@@FreshfromtheFarmFungi thanks, i always used LME DEX , this is much clearer
@Zayskibop
2 жыл бұрын
With regards to matsutake, I’ve noticed my mycorrhizal cultures always contaminate! Hydnum, Boletaceae, Amanita, etc. Any similarities for yourself? Haven’t tried any Morchella, or them on MEA or some other agar for that matter..
@FreshfromtheFarmFungi
2 жыл бұрын
they are definitely difficult - some bacteria help in nature so it probably has a more scientific reason but I want to isolate, expand and innoculate
@pineapplepileus8719
2 жыл бұрын
Hello
@mhanyvelasco6393
2 жыл бұрын
were can i buy it
@FreshfromtheFarmFungi
2 жыл бұрын
etsy.com/shop/freshfungi
@glenw3814
2 жыл бұрын
👍🍄💖
@kartalbaba981
2 жыл бұрын
👍👌👏
@patrickcollins5752
2 жыл бұрын
I wish you would make your videos in a quiter environment
@FreshfromtheFarmFungi
2 жыл бұрын
maybe someday I can have a studio for videos 👍 until then I use the lab and it’s being filtered 24/7
@squirlmy
2 жыл бұрын
@@FreshfromtheFarmFungi Some people! 😉
@jasontheauralnaut7268
2 жыл бұрын
Dude... you're just making up words
@FreshfromtheFarmFungi
2 жыл бұрын
all words are made up
@jasontheauralnaut7268
2 жыл бұрын
@@FreshfromtheFarmFungi that is a valid point. And, all words are just letters
@ridgefarmer
2 жыл бұрын
Strategery…
@squirlmy
2 жыл бұрын
@@ridgefarmer It doesn't take a rocket surgeon to figure it out!
@squirlmy
2 жыл бұрын
@@FreshfromtheFarmFungi IDK Have you read any Chomsky? There's many variations on papa and mama, but just about everyone recognizes that pa, or pop, or papa is male and mama is female, which suggests language isn't completely "made up". Oh look, you've got me started on this! Please, never mind!
@fattymcbastard6536
Жыл бұрын
Matsutake culture? Good luck with that. You might want to contact a pedologist to analyze the soil of the area you intend to grow these mushrooms. If the soil's not podzolic, you're wasting your time. Podzols are just as important as the associative plants this mushroom requires. I'm curious though, any progress so far?
@FreshfromtheFarmFungi
Жыл бұрын
interesting! I have had it grow out on agar and liquid but it’s hard to take to sawdust or wood chips indeed
@fattymcbastard6536
Жыл бұрын
@@FreshfromtheFarmFungi It will grow on agar and in LCs because of the available sugars. Naturally, these simple sugars are provided by the plants that the hyphae are tapped into, and I have no idea what the podzol layer provides for them. In the lab, you wont be able to re-create podzolic soil, as that takes years of chemical interactions from the "tea" that filters through the humous layer reacting with whatever necessary minerals in the bedrock beneath. So there's that, and the need for the plants that it grows in symbiosis with (Douglas fir, pine, prince's pine, bearberry, kinnikinnik, & evergreen huckleberry to name a few). The Japanese have been consuming these (well, the brown variety anyhow) for over a thousand years. I reckon they've given up on trying to cultivate them. And the way I see things, if you do indeed find an area with all the right conditions and plants, Matsutake will already be growing there. But hey... find a spot in the right area where there is no culture, dump a few gallons of LC around, and maybe you'll get lucky!
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