In this video I perform trajectory analysis in R on a large dataset of cells undergoing dedifferentiation into iPSCs. I use Seurat to load, merge, and preprocess the data. I use Monocle3 to calculate pseudotime and create the trajectories. I go over basic analyses and plotting using Monocle3.
Notebook:
github.com/mousepixels/sanbom...
References:
www.cell.com/cell/fulltext/S0...
cole-trapnell-lab.github.io/m...
satijalab.org/seurat/
Негізгі бет Ғылым және технология Single-cell trajectory and pseudotime analysis with Monocle3 and Seurat in R
Пікірлер: 24